We propose an innovative three-dimensional force sensing device fabricated with carbon nanofiber (CNF) polymer composites. The device has four piezoresistive strain sensors made onto a polyimide substrate using surface patterning treatment and tilted-drop process with CNF polymer solutions. The proposed design and fabrication process is simpler than that of other three-dimensional force sensors and the device is suitable for mass production. The fabricated strain sensor properties using CNF polymer solutions with different composition ratios were investigated. An equation was derived using simple percolation theory to predict the conductivity of CNF polymer composites. The measured gauge factors were in the 4.84 to 17.68 range for CNF polymer composites with CNF 8.85–45.2 wt %. A programmable system on chip (PSoC) with built-in operational (OP) amplifier, analog-to-digital (AD) converter and multiplexer was used to develop a scanning and analyzing circuit for the three-dimensional force sensing device. The proposed integrated system was successfully applied to control a computer screen cursor.