This thesis describes the construction and analysis of a first Brassica oleracea pangenome. It also presents a pipeline that was developed and implemented in order to build and analyse the pangenome.B. oleracea is a species of high economic importance encompassing crops like broccoli, Brussels sprout, cabbage, cauliflower, kale and kohlrabi. Additionally, it is of high interest to the scientific community because the crops display remarkable morphological and physiological variation and the genetic underpinning of this variation is not fully understood.Recent years have seen an explosion of genome sequencing projects. It has also become clear that there is a significant amount of presence/absence and copy number variation between individuals of the same species, and a reference sequence from a single individual is unable to capture all of the genomic information necessary to fully characterize a species. The term pangenome was coined to describe all the genomic (genic) sequence information found within species.We have hypothesized that some of the phenotypic variation found in the B. oleracea crops might be due to presence/absence variants and therefore we have set out to construct and analyse the B. oleracea pangenome.The pipeline designed was composed of three main steps 1. Pangenome assembly using an iterative mapping and assembly approach and pangenome annotation. Chalhoub, B., Denoeud, F., Liu, S., Parkin, I.A.P., Tang, H., Wang, X., Chiquet, J., Belcram, H., Tong, C., Samans, B., Corréa, M., Da Silva, C., Just, J., Falentin, C., Koh, C.S., Le Clainche, I., Bernard, M., Bento, P., Noel, B., Labadie, K., Alberti, A., Charles, M., Arnaud, D., Guo, H., Daviaud, C., Alamery, S., Jabbari, K., Zhao, M., Edger, P.P., Chelaifa, H., Tack, D., Lassalle, G., Mestiri, I., Schnel, N., Le Paslier, M.-C., Fan, G., Renault, V., Bayer, P.E., Golicz, A.A., Manoli, S., Lee, T.-H., Thi, V.H.D., Chalabi, S., Hu, Q., Fan, C., Tollenaere, R., Lu, Y., Battail, C., Shen, J., Sidebottom, C.H.D., Wang, X., Canaguier, A., Chauveau, A., Bérard, A., Deniot, G., Guan, M., Liu, Z., Sun, F., Lim, Y.P., Lyons, E., Town, C.D., Bancroft, I., Wang, X., Meng, J., Ma, J., Pires, J.C., King, G.J., Brunel, D., Delourme, R., Renard, M.,