Flocculating performances of exopolysaccharides produced by a halophilic bacterial strain cultivated on agro-industrial waste

Sam, Serdar; Küçükaşik, Faruk; Yenigün, Orhan; Nicolaus, Barbara; Öner, Ebru Toksoy; Yükselen, Mehmet Ali

doi:10.1016/j.biortech.2010.09.020

Cited by 104 publications

(37 citation statements)

References 35 publications

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“…isolated from activated-sludge was investigated (Cheng et al 2004 ;Sheng et al 2006 ). Sam et al ( 2011 ) described the biofl occulating activity of exopolymeric substances (EPSs) produced by halophilic bacterium Halomonas sp. AAD6.…”

Section: Biofl Occulantsmentioning

confidence: 99%

Halophilic Bacteria: Potentials and Applications in Biotechnology

Mohammadipanah

Hamedi

Dehhaghi

2015

Sustainable Development and Biodiversity

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Halophilic bacteria are categorized based on their requirements to salt. They apply some strategies in adaptation to the high saline environment. Their low nutritional requirements and resistance to high concentrations of salt, introduce as potent agents in wide range of biotechnological applications. Halophilic bacteria are very divergent and more than 150 species introduce in 70 genera of halophilic bacteria are reported. Use of resistant microorganisms and their metabolites under harsh industrially conditions is a strikingly important subject. Various halophilic enzymes in different enzymatically processes, compatible solutes as macromolecule stabilizers, biopolymers, biofertilizers and pharmaceutically active molecules from halophilic bacteria are among the important applications of these group. Additionally, they have many potential in bioremediation of various organic and inorganic pollutants from environment. This chapter covers the different aspects of potential applications of halophilic bacteria in biotechnology. Keywords IntroductionMicrobial life exists in hypersaline habitat refl ects the adaption of microorganisms to high concentration of salts. Halophilic and halotolerant organisms are found in all three domains of life: Archaea , Bacteria , and Eukarya . According to the requirement

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Section: Biofl Occulantsmentioning

confidence: 99%

Halophilic Bacteria: Potentials and Applications in Biotechnology

Mohammadipanah

Hamedi

Dehhaghi

2015

Sustainable Development and Biodiversity

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“…Further research on the potential uses of levan produced by Halomonas sp. as a bioflocculating agent (7), its nanostructured thin films (9), its suitability for peptide-based drug nanocarrier systems (8), and a scheme for economical production from waste (4) have been reported. Moreover, the first genome scale metabolic model of Chromohalobacter salexigens DSM 3043 was reported, which in turn formed the basis for the reconstruction of a generic in silico metabolic model of levan-producing Halomonas sp.…”

Section: H Alomonas Smyrnensis Aad6mentioning

confidence: 99%

Draft Genome Sequence of Halomonas smyrnensis AAD6 ^T

et al. 2012

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Turkey b Halomonas smyrnensis AAD6T is a Gram-negative, aerobic, exopolysaccharide-producing, and moderately halophilic bacterium that produces levan, a fructose homopolymer with many potential uses in various industries. We report the draft genome sequence of H. smyrnensis AAD6 T , which will accelerate research on the rational design and optimization of microbial levan production. H alomonas smyrnensis AAD6T was isolated from soil samples taken from the Çamaltı Saltern area (Turkey) and was found to excrete high levels of levan exopolysaccharide (5). Further research on the potential uses of levan produced by Halomonas sp. as a bioflocculating agent (7), its nanostructured thin films (9), its suitability for peptide-based drug nanocarrier systems (8), and a scheme for economical production from waste (4) have been reported. Moreover, the first genome scale metabolic model of Chromohalobacter salexigens DSM 3043 was reported, which in turn formed the basis for the reconstruction of a generic in silico metabolic model of levan-producing Halomonas sp. (1). Recently, H. smyrnensis strain AAD6T was identified as a Gram-negative, nonmotile, dark-yellow-pigmented, aerobic, moderately halophilic bacterium (6).In this study, whole-genome sequencing of H. smyrnensis AAD6 T was performed by using a combination of 454 GS FLXϩ (454 Life Sciences, Branford, CT) and Ion Proton (Ion Torrent Systems, Inc., Guilford, CT) sequencers. The shotgun sequence data generated consisted of 1,082,564 reads (mean length of 498.36 bp) and 359,877 reads (mean length of 122.42 bp), respectively, providing 140-fold coverage. The duplicates in the sequence data were eliminated with CLC Genomics Workbench (v5.0.1). De novo assembly of the whole sequencing reads was carried out with Newbler Assembler (v2.3). One hundred ninetytwo contigs, with a minimum length of 500 bp, were obtained. Alignment of these contigs and elimination of the overlaps using Sequencher 5.0 (3) resulted in 81 high-quality contigs. These contigs were mapped to the raw data with CLC Genomics Workbench (v5.0.1) to estimate the terminals, and using overlapping terminal regions, the contigs were assembled into 34 supercontigs via Geneious Pro (v5.5.6) software. The draft genome of H. smyrnensis AAD6T was structured with 34 supercontigs (total assembly size of 3,561,919 bp). Gene prediction and annotation were carried out using the RAST server (2).The draft genome that resulted had a mean GC content of 67.9%, 3,237 coding sequences, 1,756 subsystems, and 64 RNA genes. Putative functions could be assigned to 2,436 protein-coding genes, whereas 801 hypothetical proteins had no match to any known proteins. Genome analysis revealed a number of genes related to exopolysaccharide biosynthesis, including the genes for levansucrase and ExoD.Phylogenetic analysis of the draft genome revealed a rather close relationship to other species of the genus Halomonas, where Halomonas salina (99.5%) and Halomonas halophila (99.5%) were found to be the closest species on the basis of 16S rRNA sequ...

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“…Rajan et al found that Trichococcus flocculiformis and Pseudomonas fluorescens isolated from polluted tannery soil had flocculation ability [10]. Serdar et al found there was polysaccharide in bioflocculant used to treat tannery wastewater [11]. Despite these findings, the higher cost or lower flocculation efficiency have limited application of these organisms for industrial processes [12,13].…”

Section: Introductionmentioning

confidence: 99%

Screening of Bioflocculant and Preliminary Application to Treatment of Tannery Wastewater

Yang¹,

Ming²,

Zhao³

et al. 2015

JRST

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ABSTRACT:In this study, three strains of bacteria Bacillus cereus CZ1001, B. subtilis CZ1002, and B. fusiformis CZ1003 were inoculated into four different kinds of culture medium. After growth, the supernatants were collected and added to kaolin suspensions to determine whether these Bacillus strains produced flocs that could be filtered out. Efficient bacteria and media were then selected to produce bioflocculants which were subsequently extracted using pre-cooled ethanol. After freeze drying, the flocculants were used to treat tannery wastewater. Results showed cultures of B. subtilis CZ1002 growing in media No. 4, B. cereus CZ1001 growing in media No.

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Flocculating performances of exopolysaccharides produced by a halophilic bacterial strain cultivated on agro-industrial waste

Cited by 104 publications

References 35 publications

Halophilic Bacteria: Potentials and Applications in Biotechnology

Halophilic Bacteria: Potentials and Applications in Biotechnology

Draft Genome Sequence of Halomonas smyrnensis AAD6 ^T

Screening of Bioflocculant and Preliminary Application to Treatment of Tannery Wastewater

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Flocculating performances of exopolysaccharides produced by a halophilic bacterial strain cultivated on agro-industrial waste

Cited by 104 publications

References 35 publications

Halophilic Bacteria: Potentials and Applications in Biotechnology

Halophilic Bacteria: Potentials and Applications in Biotechnology

Draft Genome Sequence of Halomonas smyrnensis AAD6 T

Screening of Bioflocculant and Preliminary Application to Treatment of Tannery Wastewater

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Product

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Draft Genome Sequence of Halomonas smyrnensis AAD6 ^T