Flocculation mechanism of Aspergillus niger on harvesting of Chlorella vulgaris biomass

Li, Yang; Xu, Yanting; Liu, Lei; Ping, Li; Yan, Yue; Chen, Tong; Zheng, Tao; Wang, Hailei

doi:10.1016/j.algal.2017.06.001

Cited by 58 publications

(30 citation statements)

References 28 publications

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“…Complete removal of algal cells from the water phase was observed after three days of fungal growth and high efficiency in algal harvesting was achieved, which is well in line with previous findings on the use of A. niger for this purpose [4]. A recent study suggests that specific surface proteins in the hyphae of fungi are important for achieving algal pelleting [18].…”

Section: Discussionsupporting

confidence: 89%

Impact on Wastewater Quality of Biopellets Composed of Chlorella vulgaris and Aspergillus niger and Lipid Content in the Harvested Biomass

Hultberg¹,

Bodin²,

Birgersson³

2019

JWARP

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Filamentous fungi can be used to form easily harvested pellets with microalgae (fungal-assisted algal harvesting) in order to advance the sustainability and economic feasibility of wastewater treatment using microalgae. In experiments employing the microalga Chlorella vulgaris and using the filamentous fungus Aspergillus niger for harvesting, this study investigated the effect on water quality and the quantity and quality of lipids in the biomass produced. Major reductions in the concentrations of total nitrogen, ammonium-nitrogen and total phosphorus were observed after addition of the fungal spores (day 5) and during fungal growth and entrapment of the algal cells. At harvest (day 8), the decrease in total nitrogen was 47.4% ± 18.4% of the initial value, corresponding to a reduction of 41.9 ± 17.1 mg•nitrogen•L −1. For total phosphorus, the decrease was 94.4% ± 3.2%, corresponding to a reduction of 6.4 ± 0.2 mg•phosphorus•L −1. A significant decrease in concentration of the micropollutant diclofenac was observed at harvest, to 5.1 ± 4.0 µg•L −1 compared with an initial concentration of 9.5 ± 0.6 µg•L −1. A significant decrease in total lipids in the biomass was observed after fungal-assisted algal harvesting, from 58.7 ± 2.7 µg•mg −1 at day 5 (algal biomass only) to 34.2 ± 2.7 µg•mg −1 at day 8 (fungal-algal biomass). However, because of high biomass production, the amount of lipids produced per litre of wastewater increased from 5.6 ± 0.9 mg on day 5 to 20.6 ± 4.9 mg on day 8.

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Section: Discussionsupporting

confidence: 89%

Impact on Wastewater Quality of Biopellets Composed of Chlorella vulgaris and Aspergillus niger and Lipid Content in the Harvested Biomass

Hultberg¹,

Bodin²,

Birgersson³

2019

JWARP

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“…Acid and alkali treatments can induce proteins denaturation [ 18 ]. Li et al have reported flocculation activity of mycelial pellets of Aspergillus niger after treated by acid (pH 3) and alkali (pH 12) treatment [ 19 ], which showed that flocculation activities in acid and alkali treatment groups were significantly ( p < 0.01) lower than that in positive control, suggesting proteins are major factors in the flocculation process. Lei et al have reported that bioflocculant L03 was stable within a wide range of pH values, between 4 and 10 [ 15 ], suggesting proteins maybe not major factor to induce flocculation activity.…”

Section: Discussionmentioning

confidence: 99%

“…Li et al have confirmed flocculation activity of mycelial pellets incubated with proteinase K was significantly ( p < 0.01) lower than that of the nondigested controls [ 19 ], indicating that proteinase K-cleavable surface proteins are major factors in the flocculation process. Therefore, proteins in bioflocculant did not play any flocculation effect to harvest algal cells.…”

Section: Discussionmentioning

confidence: 99%

Flocculation characteristics of a bioflocculant produced by the actinomycete Streptomyces sp. hsn06 on microalgae biomass

Rui-xue

et al. 2018

BMC Biotechnol

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BackgroundMicrobial flocculation is a good choice for harvest of microalgae biomass, which has gained extensive attention. There have been carried out massive studies in bacterial flocculation, many bacterial strains with flocculation activity were isolated and different types of bioflocculants were produced. However, harvest of algal biomass by bioflocculants which produced from actinomycete are deficiency. In this study, the bioflocculant from an actinomycete Streptomyces sp. hsn06 could be used to harvest Chlorella vulgaris biomass.ResultsConsecutive treatment with 20 mg·L− 1 bioflocculant and 5 mM CaCl2 for 5 min showed the highest flocculating activity. The bioflocculant was a nonprotein substance with thermal stability and pH stability, which can be used in comprehensive applications. Chemical analysis of the bioflocculant indicated that it is a small molecule substance of moderate polarity with containing triple bond and cumulated double bonds. Algal temperature, pH, and metal ions showed great effects on the flocculation efficiency of the bioflocculant.ConclusionsThe bioflocculant produced by Streptomyces sp. hsn06 possesses the potential to harvest algal biomass with high-efficiency.Electronic supplementary materialThe online version of this article (10.1186/s12896-018-0471-9) contains supplementary material, which is available to authorized users.

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“…Hence, there is an immediate requirement to develop an efficient algal dewatering process to reach the biomass up to 30% in total dry product. Concentrating algal biomass and purifying it into products from broth occur in two stages: a single step of harvesting followed by one-or two-step separate biomass dewatering, which is then fractionated and extracted to extend the "shelf-life of biomass" and to make the product accessible for further application [58,59].…”

Section: Biomass Harvesting and Dewateringmentioning

confidence: 99%

Contribution of Anaerobic Digestion Coupled with Algal System towards Zero Waste

Machineni¹,

Rao²,

Rao³

2021

Biogas - Recent Advances and Integrated Approaches

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Global environmental protection is of immediate concern and it can only be achieved by avoiding the use of fossil fuels. In addition, waste disposal and management could be made remunerative through the generation of renewable energy so that sustainable development is ensured. India is an agriculture-based country, and paddy residues such as rice straw and rice husk are the largest agricultural wastes in India. Currently, the common practice to dispose paddy residues is through field burning, but this has adverse effects on the air quality and consequently on people's health. However, utilization of lignocellulosic and non-food agricultural residues such as paddy residue for biogas generation by solid-stated anaerobic digestion (AD) is promising and this can substitute fossil fuels. Paddy residues for biogas production via AD has not been widely adopted because of its complex cell wall structure making it resistant to digestion by microbial attack. In addition, sequestration of carbon dioxide from biogas by algal biomass cultivated in an integrated algal bioreactor could be a promising option for biogas enrichment due to its unmatched advantages. This chapter presents the overview on utilization of non-edible residues for biogas production and its enrichment via algal biomass by means of circular bioeconomy.

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Flocculation mechanism of Aspergillus niger on harvesting of Chlorella vulgaris biomass

Cited by 58 publications

References 28 publications

Impact on Wastewater Quality of Biopellets Composed of <i>Chlorella vulgaris</i> and <i>Aspergillus niger</i> and Lipid Content in the Harvested Biomass

Impact on Wastewater Quality of Biopellets Composed of <i>Chlorella vulgaris</i> and <i>Aspergillus niger</i> and Lipid Content in the Harvested Biomass

Flocculation characteristics of a bioflocculant produced by the actinomycete Streptomyces sp. hsn06 on microalgae biomass

Contribution of Anaerobic Digestion Coupled with Algal System towards Zero Waste

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Flocculation mechanism of Aspergillus niger on harvesting of Chlorella vulgaris biomass

Cited by 58 publications

References 28 publications

Impact on Wastewater Quality of Biopellets Composed of &lt;i&gt;Chlorella vulgaris&lt;/i&gt; and &lt;i&gt;Aspergillus niger&lt;/i&gt; and Lipid Content in the Harvested Biomass

Impact on Wastewater Quality of Biopellets Composed of &lt;i&gt;Chlorella vulgaris&lt;/i&gt; and &lt;i&gt;Aspergillus niger&lt;/i&gt; and Lipid Content in the Harvested Biomass

Flocculation characteristics of a bioflocculant produced by the actinomycete Streptomyces sp. hsn06 on microalgae biomass

Contribution of Anaerobic Digestion Coupled with Algal System towards Zero Waste

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Impact on Wastewater Quality of Biopellets Composed of <i>Chlorella vulgaris</i> and <i>Aspergillus niger</i> and Lipid Content in the Harvested Biomass

Impact on Wastewater Quality of Biopellets Composed of <i>Chlorella vulgaris</i> and <i>Aspergillus niger</i> and Lipid Content in the Harvested Biomass