Floral anatomy, ultrastructure and chemical analysis in Dactylorhiza incarnata/maculata complex (Orchidaceae)

Naczk, Aleksandra M.; Kowalkowska, Agnieszka K.; Wiśniewska, Natalia; Haliński, Łukasz P.; Kapusta, Małgorzata; Czerwicka, Małgorzata

doi:10.1093/botlinnean/boy027

Cited by 19 publications

(22 citation statements)

References 79 publications

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“…Samples were prepared for the above mentioned observations in accordance with previously described procedures (i.e. Kowalkowska et al 2010; Krawczyk et al 2016; Święczkowska and Kowalkowska 2015; Naczk et al 2018). The sections were examined in a Tecnai G2 Spirit BioTwin FEI transmission electron microscope (Laboratory of Electron Microscopy, University of Gdańsk, Poland) at an accelerating voltage of 120 kV.…”

Section: Methodsmentioning

confidence: 99%

Labellum structure of Bulbophyllum echinolabium J.J. Sm. (section Lepidorhiza Schltr., Bulbophyllinae Schltr., Orchidaceae Juss.)

et al. 2019

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This micromorphological, chemical and ultrastructural study is a continuation of research conducted on the section Lepidorhiza . The Bulbophyllum echinolabium flowers comprised features that characterize a sapromyophilous syndrome, having large, glistening parts that emit an intense scent of rotten meat. The secretory activity was described in the hypochile (nectary in longitudinal groove and in the prickles) and the epichile (putative osmophore). The ultrastructural studies revealed a dense cytoplasm in the epidermis and subepidermal tissue with large nuclei and numerous mitochondria, the profiles of SER and RER, and dictyosomes. Large amounts of heterogeneous residues of secreted material (possibly phenolic) were present on the cuticle surface, similar to the unusual prominent periplasmic space with flocculent secretory material. The chemical analysis (GC/MS) of the scent profile of lips comprised carbohydrates and their derivatives (29.55% of all compounds), amino acids (1.66%), lipids (8.04%) and other organic compounds (60.73%). A great number of identified compounds are Diptera attractants (mainly Milichiidae, Tephritidae, Drosophilidae, Muscidae, Sarcophagidae, Tachinidae). The examination of visual and olfactory features indicates correlation between colour of flowers and the type of olfactory mimicry, where a dark colour labellum emits strong smell of rotten waste.

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Section: Methodsmentioning

confidence: 99%

Labellum structure of Bulbophyllum echinolabium J.J. Sm. (section Lepidorhiza Schltr., Bulbophyllinae Schltr., Orchidaceae Juss.)

et al. 2019

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“…2019 ), or may have papillae and/or multi- or unicellular trichomes ( Curry et al . 1991 ; Davies and Turner 2004 ; Naczk et al . 2018 ).…”

Section: Discussionmentioning

confidence: 99%

Coryanthes macrantha(Orchidaceae: Stanhopeinae) and their floral and extrafloral secretory structures: an anatomical and phytochemical approach

et al. 2022

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Coryanthes is one of the most fascinating genera of Stanhopeinae (Orchidaceae) because of its complex pollination mechanism and the peculiar structures of its flowers. Although Coryanthes macrantha (Hook.) Hook. is widely studied, investigation of the secretory structures and floral biology is important to understand the mechanisms and ecology of pollination, which deserve attention despite the difficulties of collecting fertile material in nature. We conducted a morpho-anatomical analysis of the floral and extrafloral secretory structures of Coryanthes macrantha to better understand the secretory structures, contribute to the knowledge of its floral biology and/or pollination processes, and understand the ecological function of these structures. The analysis revealed that C. macrantha has epidermal osmophores with unicellular papillae that were foraged by male Eulaema bees, floral nectaries in the sepals, and extrafloral nectaries in the bracts. In both the floral and extrafloral nectaries, the nectar is exuded by the stomata. Azteca ants foraged the bract and sepal nectaries in pre-anthesis and post-anthesis. We also described the secretory epidermis of pleuridia, and the mode of secretion of osmophores and nectaries and found that they attract specific foraging agents.

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“…Presence of the irregular plasmalemma, with vesicles building into it, has been associated with granulocrine secretion and widely described in different secretory cells (Fahn 1988;Stpiczy nska 2001;Kowalkowska et al 2012Kowalkowska et al , 2015Naczk et al 2018;Paiva et al 2022). The secretory products then accumulate in the periplasmic space, diffuse through the cell walls, and accumulate beneath the cuticle that gradually separates from the cell wall.…”

Section: Cytological Events Linked To the Secretory Processmentioning

confidence: 99%

“…Hydrophilic substances gradually diffuse out of the cells through a cuticle electron-dense reticulation that functions as microchannels. The involvement of cuticle microchannels in the apoplastic transport of substances through the cuticle to the trichome surface has been described in different glands of other plant species (Kowalkowska et al 2012(Kowalkowska et al , 2015Guimarães et al 2018;Naczk et al 2018).…”

Section: Cytological Events Linked To the Secretory Processmentioning

confidence: 99%

Peltate trichomes in the dormant shoot apex of Metrodorea nigra, a Rutaceae species with rhythmic growth

et al. 2022

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In Metrodorea nigra, a Rutaceae species with rhythmic growth, the shoot apex in the dormant stage is enclosed by modified stipules. The young organs are fully covered with peltate secretory trichomes, and these structures remain immersed in a hyaline exudate within a hood-shaped structure. Our study focused on the morpho-functional characterization of the peltate trichomes and cytological events associated with secretion.• Shoot apices were collected during both dormant and active stages and processed for anatomical, cytochemical and ultrastructural studies.• Trichomes initiate secretion early on, remain active throughout leaf development, but collapse as the leaves expand; at which time secretory cavities start differentiation in the mesophyll and secretion increases as the leaf reaches full expansion. The subcellular apparatus of the trichome head cells is consistent with hydrophilic and lipophilic secretion. Secretion involves two vesicle types: the smaller vesicles are PATAg-positive (periodic acid/thiocarbohydrazide/silver proteinate) for carbohydrates and the larger ones are PATAg-negative. In the first phase of secretory activity, the vesicles containing polysaccharides discharge their contents through exocytosis with the secretion accumulating beneath the cuticle, which detaches from the cell wall. Later, a massive discharge of lipophilic substances (lipids and terpenes/phenols) results in their accumulation between the wall and cuticle. Release of the secretions occurs throughout the cuticular microchannels.• Continued protection of the leaves throughout shoot development is ensured by replacement of the collapsed secretory trichomes by oil-secreting cavities. Our findings provide new perspectives for understanding secretion regulation in shoot apices of woody species with rhythmic growth.

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Floral anatomy, ultrastructure and chemical analysis in Dactylorhiza incarnata/maculata complex (Orchidaceae)

Cited by 19 publications

References 79 publications

Labellum structure of Bulbophyllum echinolabium J.J. Sm. (section Lepidorhiza Schltr., Bulbophyllinae Schltr., Orchidaceae Juss.)

Labellum structure of Bulbophyllum echinolabium J.J. Sm. (section Lepidorhiza Schltr., Bulbophyllinae Schltr., Orchidaceae Juss.)

Coryanthes macrantha(Orchidaceae: Stanhopeinae) and their floral and extrafloral secretory structures: an anatomical and phytochemical approach

Peltate trichomes in the dormant shoot apex of Metrodorea nigra, a Rutaceae species with rhythmic growth

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