This study compares the plant structure of native and passive regenerated grassland areas in the Vila Velha State Park, southern Brazil. We analyzed four data set and observed that: (i) areas former submitted both to agricultural activity and fire showed lower species diversity (mainly Poaceae) and three abundant taxa (the ruderale grasses Eragrostis airoides, Andropogon bicornis, and Eustachys distichophylla); (ii) in grasslands former cultivated but not burned for the last 10 years, species diversity was slight higher and abundant taxa included the grasses Andropogon bicornis, Paspalum distichum, and Paspalum paspalodes, and the herbs Pteridium arachnoideum and Baccharis dracunculifolia; (iii) in native grasslands, never used for agriculture but recently burned, species diversity is higher but abundant taxa were just the grasses Aristida jubata and Paspalum rhodopedum; (iv) in native grasslands never cultivated nor burned for the last 10 years, species diversity was much higher and abundant taxa were the herbs Grazielia gaudichaudiana, Stevia clausenii, Chrysolaena platensis, and Lessingianthus grandiflorus. Additionally, typical grassland taxa as Chromolaena verbenacea, Allagoptera campestris, Croton antisiphyliticus, Cayaponia espelina, Leandra erostrata, and Byttneria hatschbachii have been found only in the undisturbed area. Thus, the areas showed diverse floristic composition, yet their biodiversity metrics and functional groups did not differ significantly. In summary, despite agriculture and fire decreasing the park species diversity, the base level for spontaneous recovery process did not reach thresholds that would prevent the maintenance of community structure.