Flow cytometric analysis of cell cycle fractions in oral leukoplakia

Model of Chromosomal Instability in Oral Carcinogenesis and Progression

“…The data obtained for the nondysplastic OPMLs, in particular, were in agreement with previous literature reports including two studies from our group using an independent patient population (Donadini et al, 2010;Pentenero et al, 2009;Saito, 1995). These data were, however, in contrast with other studies, which did not detect DNA aneuploid sublines in such lesions (Kahn et al, 1992;Saito, 1998). The present study has additionally highlighted that ODFs and OPMLs without dysplasia were characterized by single near-diploid DNA aneuploid sublines.…”

“…The incidence of DNA aneuploidy by FCM reported in the literature ranges from about 10% to 40% for dysplastic OPMLs (Donadini et al 2010;Pentenero et al 2009;Saito et al, 1998Saito et al, , 1991Saito et al, , 1995Seoane et al, 1998) and up to about 80% for OSCCs (Donadini et al, 2010;Pentenero et al 2009;Hemmer, 1990Hemmer, , 1997. These values may strongly depend on the tissue material type (paraffin embedded or fresh-frozen) and DNA FCM resolution.…”

“…The role of chromosomal instability during the genesis and progression of oral cancer has clearly been indicated by several studies but still our understanding of the molecular mechanisms is relatively poor. Analyses were performed with the use of different techniques including loss of heterozygosity (Braakhuis et al, 2004;Bremmer et al, 2008;Graveland et al, 2011;Lydiatt et al, 1998;Mithani et al, 2007;Partridge et al, 1997;Tsantoulis et al, 2007), comparative genomic hybridization and gene expression array (Bremmer et al, 2008;Cha et al, 2011;Garnis et al, 2009;Liu et al, 2006Liu et al, , 2011Smeets et al, 2009;Snijders et al, 2005;Squire et al, 2002;), in situ hybridization (Nees et al, 1993;Voravud et al, 1993), immunohistochemistry (Nees et al, 1993), multiplex ligation-dependent probe amplification (Bremmer et al, 2008;Cha et al, 2011;Liu et al, 2006), DNA image cytometry (Bremmer et al, 2008;Diwakar et al, 2005) and DNA flow cytometry (DNA FCM) (Donadini et al, 2010;Hemmer, 1990Hemmer, , 1997Pentenero et al, 2009;Saito, 1998Saito, , 1991Saito, , 1995Seoane et al, 1998). DNA FCM was often adopted as a useful technique for detecting the presence of DNA aneuploid sublines in several human predisposing and preneoplastic lesions such as Barrett's esophagus (Reid et al, 2000), ulcerative colitis (Rabinovitch et al, 1999;Risques et al, 2008), colorectal adenomas (Giaretti et al, 1994) and oral lesions …”

S‐phase fraction and DNA ploidy in oral leukoplakia

“…The anueploidy rate was higher in severely dysplastic lesions than in mildly dysplastic and non‐dysplastic lesions. The SPF of the severely dysplastic lesions (23%) among the diploid leukoplakias was higher than those of the mildly dysplastic (12%) and non‐dysplastic lesions (11%) 11 . In another study, assessing the SPF of oral cancer by 3‐H, thymidine labelling index (TLI), it was found that TLI was more reliable compared with histologic description in determining the proliferative potential of dysplastic epithelial changes 12…”

DNA ploidy analysis in salivary gland tumours by image cytometry

“…Ploidy analysis by flow or image cytometry is able to detect gross genomic aberrations and has been used to determine prognosis in pre‐cancerous and cancerous lesions in the mouth, cervix and oesophagus (6–9). For example, diploid oral squamous cell carcinomas (SCC) have a better prognosis than aneuploid cases (7).…”