To evaluate prognostic and therapeutic significance, tumor DNA content was determined by flow cytometry in 310 paraffin-embedded tissue samples obtained surgically from 130 patients with non-small cell lung cancer. Ninety-six (76.8%) patients had DNA aneuploid patterns that were statistically higher in adenocarcinoma than in squamous cell carcinoma. A better 5-year survival rate was observed in Group A (DNA diploidy, 69.6%) than in Group B (DNA aneuploidy and DNA peridiploidy, 33.2%; P less than 0.001). The survival curves of the patients in Group B continued to decrease during the next 2.5 years. Cox's model analysis showed that both the pathologic stage and the DNA content were the significant prognostic factors for survival. However, the DNA content was an independent prognostic factor in squamous cell carcinoma, but not in adenocarcinoma. These results indicate that DNA content analysis is useful for the evaluation of clinical behavior and prognosis, and that the clinical value of the DNA content must be differentiated between squamous cell carcinoma and adenocarcinoma.

No abstract

Summary The chromosomal banding patterns of canine transmissible sarcoma (CTS) cells were analyzed and compared to those of normal canine cells with four banding techniques. In addition, N‐myc and N‐ras oncogenes on the chromosomes of the CTS cells were investigated by the in situ hybridization method. The modal chromosome number of the CTS cells was 58:17 metacentrics and 41 acrocentrics. Based on their G‐ and Q‐banding patterns most of the chromosomes of the CTS cells were present in normal cells except for the second largest metacentric chromosomes. It was considered that the metacentric chromosomes of the CTS cells results from Robertsonian translocation of normal chromosomes. The long arm of the second largest metacentric element of the CTS cells was stained negatively by the Q‐banding technique. The C‐band was found on the long arm of the second largest metacentric element and three pairs of N‐bands were recognized in the same region. It was suggested that this amplification of N‐bands enhanced tumorigenic properties in the CTS. No N‐myc or N‐ras oncogenes were detected on chromosomes of the CTS cells by the in situ hybridization method. Zusammenfassung Chromosomen‐Analyse von Zellen des transmissiblen Sarkoms beim Hund Bei Zellen des transmissiblen Sarkoms des Hundes (CTS) wurde das chromosomale Banden‐Muster mit vier Färbe‐Techniken analysiert und mit demjenigen normaler Zellen des Hundes verglichen. Zusätzlich wurden mit der Methode der in situ‐Hybridisierung die N‐myc‐ und N‐ras‐Onkogene auf den Chromosomen der CTS‐Zellen untersucht. Die Chromosomenzahl der CTS‐Zellen betrug 58: 17 metazentrische und 41 acrozentrische Chromosomen. Nach dem G‐ und Q‐Banden‐Muster waren mit Ausnahme des zweitgrößten metazentrischen Chromosoms die meisten Chromosomen der CTS‐Zellen in normalen Zellen vorhanden. Es wird vermutet, daß die metazentrischen Chromosomen der CTS‐Zellen aus der Robertson'schen Translokation normaler Chromosomen resultieren. Der lange Arm des zweitgrößten Elements der CTS‐Zellen färbte sich mit der Q‐Banden‐Technik negativ. Die C‐Bande wurde am langen Arm des zweitgrößten metazentrischen Elements gefunden. Ferner ließen sich drei Paare von N‐Banden am gleichen Abschnitt feststellen. Es wird angenommen, daß diese Vermehrung der N‐Banden die tumorigenen Eigenschaften der CTS‐Zellen verstärkt. Mit der Methode der in situ‐Hybridisierung konnten auf Chromosomen der CTS‐Zellen keine N‐myc‐ oder N‐ras‐Onkogene nachgewiesen werden.

No abstract

No abstract