Flow cytometric analysis of cellular DNA content in ovarian cancer

Hamaguchi, K; Nishimura, Haruo; Miyoshi, Tadashi; Miyahara, Kenichi; Tateno, Nobumasa; Yakushiji, M; Yokoyama, Mineyuki

doi:10.1016/0090-8258(90)90336-j

Cited by 21 publications

(4 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Residual tumor volume is another prognostic factor for epithelial ovarian tumors that is mostly associated with tumor aneuploidy [14], as stated in our study. Brescia et al [15] have shown that DNA analysis seems to estimate SLL results fairly well.…”

Section: Discussionsupporting

confidence: 49%

Effect of Cellular DNA Content on the Prognosis of Epithelial Ovarian Cancers

Özalp

Yalçın

Gülbaş

et al. 2001

Gynecol Obstet Invest

View full text Add to dashboard Cite

Objective: To assess the cellular DNA status of epithelial ovarian cancers with regard to clinicopathological findings and its effect on prognosis. Materials and Methods: Twenty-six consecutive patients with a diagnosis of epithelial ovarian cancer who had been treated by primary surgery and six courses of platinum-based chemotherapy were enrolled in this study. Second-look laparotomy (SLL) was performed in all cases following confirmation of the clinical remission state. Surgical stage, tumor grade, initial tumor volume, residual tumor volume, histopathologic differentiation, and SLL findings were analyzed in correlation with DNA ploidy and DNA index. DNA analysis was performed via DNA flow cytometry through paraffin-embedded tissue specimens. Results: Of 26 patients, flow cytometric studies revealed 16 aneuploidy cases (61.5%). DNA index values ranged from 1.1 to 1.82 (average 1.29 ± 0.28). The flow cytometry coefficient of variation mean value was set to 6.7. Taking the cutoff value of 1.2 for DNA indices, a fairly good correlation was detected between DNA ploidy and DNA indices (p < 0.001). The aneuploidy incidence was found to be high in advanced and poorly differentiated tumors (p < 0.05). There was statistically more residual tumor volume in aneuploid tumors during primary cytoreductive surgery and also higher recurrence rates following six courses of chemotherapy compared with diploid tumors (p < 0.05). No significant correlation was detected between the histopathologic subtypes and tumor volume (p > 0.05). Residual tumor volumes were larger in cases with DNA indices of 1.2 yielding higher residual tumor volume following surgery and being in good correlation with SLL results (p < 0.05). The mean survival rates of cases with aneuploid tumor and a DNA index of >1.2 were low compared to those with diploid tumors and DNA indices of <1.2 tumors (p < 0.05). Conclusion: DNA ploidy and DNA indices are important prognosticators for malignant epithelial ovarian tumors. They should be evaluated together with the patient’s clinical status and other prognostic factors.

show abstract

Section: Discussionsupporting

confidence: 49%

Effect of Cellular DNA Content on the Prognosis of Epithelial Ovarian Cancers

Özalp

Yalçın

Gülbaş

et al. 2001

Gynecol Obstet Invest

View full text Add to dashboard Cite

show abstract

“…Patients with TS activity over 2.0 pmol/g tissue had significantly worse prognoses than those with lower TS activity. In the past, the histologic degree of tumor differ entiation has been used as a prognostic factor, particularly in endometrial and ovarian cancers [14][15][16], More recent ly, flow-cytometric analyses of nuclear DNA content and proliferative activity of the tumor cell population have been considered useful for predicting prognosis [17][18][19][20][21], In our study, DNA aneuploidy and a high S-phase frac tion were correlated with poor prognoses; however, no sig nificant difference was seen between groups with high and low S-phase fractions or aneuploid and diploid tumors, probably because of the small study population and the short observation period in this study. These results sug gest that TS activity may be more useful than flow-cyto metric analysis of DNA content for evaluating the biologi cal behavior of ovarian carcinoma, and for predicting the prognosis of patients with ovarian cancer.…”

Section: Discussionmentioning

confidence: 99%

Thymidylate Synthase Activity as a Prognostic Factor in Ovarian Cancer

Suzuki

Ohwada²,

Tamada³

et al. 1994

Oncology

View full text Add to dashboard Cite

We assayed thymidylate synthase (TS) activity and performed flow-cytometric DNA analyses in fresh tumor tissues from 38 patients with ovarian cancer. TS activity was closely correlated with prognosis but was not related to age, clinical stage, histologic type or lymph node metastases. Patients with TS activity higher than 2.0 pmol/g tissue had significantly worse prognoses than those with lower levels (p < 0.01). Also, patients with DNA aneuploidy or high S-phase fractions ( > 20%) had worse prognoses than those with DNA diploidy or lower fractions. Results suggest that TS activity may be a good prognostic indicator in ovarian cancer.

show abstract

“…This multiple site sampling demonstrated that single biopsy specimens, when used in karyotyping, DNA FCM, and interphase cytogenetics on nuclear suspensions, are hardly representative for a given (prostatic) tumor (4,7,31,37,38). At present, the clinical importance of DNA ploidy heterogeneity is not clear and varies among the tumours studied (10,14,15).…”

Section: Discussionmentioning

confidence: 99%

“…Both aneuploidy/tetraploidy and diploidy have been detected when several biopsies per tumor were analyzed (4,24,31). Likewise, FCM DNA studies of multiple samples from different sites in one tumor and/or metastases have shown heterogeneity in DNA in lung cancers (7,38), gliomas (lo), pancreatic tumors (39), gastrointestinal cancers ( 11,38), and ovarian carcinomas ( 15). In addition, in epithelial tumors such as bladder cancer (19,20,35), breast cancer ( 1 2 ) , and lung cancer ( 2 2 ) , cytogenetic heterogeneity has been revealed by interphase cytogenetics.…”

mentioning

confidence: 99%

Cytogenetic heterogeneity and histologic tumor growth patterns in prostatic cancer

et al. 1995

View full text Add to dashboard Cite

Twenty-five prostatic adenocarcinomas were studied for the presence of intratumoral cytogenetic heterogeneity by interphase in situ hybridization (ISH) to routinely processed tissue sections. ISH with a chromosome Y-specific repetitive DNA probe provided a model to investigate patterns of chromosomal heterogeneity within and between different pathological grades. The Gleason grading system was used, since it is based on a detailed classification of growth patterns. Heterogeneity with respect to ploidy of the tumor was examined by ISH with a repetitive DNA probe specific for chromosome 1. The ploidy status of these cancers was confirmed by DNA flow cytometry (P < 0.001). Cytogenetic heterogeneity at the (Y) chromosomal level was observed between Gleason areas, within one area, and even within single tumor glands. The different patterns of chromosomal heterogeneity were seen in all tumor grades and stages. Differences in ploidy status were also found following the aforementioned histological patterns, again, in all grades and stages. Intraglandular heterogeneity was most frequently seen. No correlation was found between cytogenetic heterogeneity and proliferative activity . In contrast to current views on clonality, suggesting regional separation of subclones with different DNA content, this study demonstrates that these subclones can be interspersed. o 1995 wiley-Liss, h c .

show abstract

Flow cytometric analysis of cellular DNA content in ovarian cancer

Cited by 21 publications

References 13 publications

Effect of Cellular DNA Content on the Prognosis of Epithelial Ovarian Cancers

Effect of Cellular DNA Content on the Prognosis of Epithelial Ovarian Cancers

Thymidylate Synthase Activity as a Prognostic Factor in Ovarian Cancer

Cytogenetic heterogeneity and histologic tumor growth patterns in prostatic cancer

Contact Info

Product

Resources

About