Flow cytometric analysis of human dental pulp tissue

Phenotypic Analysis of Immunocompetent Cells in Healthy Human Dental Pulp

“…Data concerning the quantification of T lymphocytes in human dental pulp are inconsistent in the literature. Our results are in agreement with those of previous immunohistologic studies (7,8,13) but in contrast with those from flow cytometry analysis in which CD4 + T lymphocytes were 1.2 times more numerous than CD8 + T lymphocytes (12,19). This discrepancy could be caused by differences in the selection method of T lymphocytes.…”

“…In the field of endodontic research, Mangkornkarn et al (12) were the first to use FACS to examine the cellular components of human dental pulp tissue. FACS is a highly sensitive tool for cell counting compared with immunolabeling of histologic sections (7,8,(12)(13)(14).…”

“…FACS is a highly sensitive tool for cell counting compared with immunolabeling of histologic sections (7,8,(12)(13)(14). FACS analysis gives the opportunity to combine several antibodies and to use positive or negative selection methods to identify and quantify specific cell populations in a reliable way (15).…”

Immune Defense Mechanisms of the Dental Pulp

“…Although numerically minor among pulpal cells, these T-lymphocytes are found to be scattered predominantly along the blood vessels in the inner portion of the pulp. Flow cytometric analysis of cells from the human pulp has also demonstrated CD4+ and CD8+ cells, while more CD4+ cells were detected than CD8+ cells (Mangkornkarn et al, 1991). Recently, Tlymphocytes expressing CD45RO have been identified in the human pulp (Izumi et al, 1995).…”

“…Several researchers have failed to identify B-lymphocytes in the human dental pulp by the use of immunohistochemistry with antisera against immunoglobulins (Pulver et al, 1977;Pekovic and Fillery, 1984), or with monoclonal antibodies against B-lymphocytes (jontell Mangkornkarn et al, 1991;Sakamoto and Sanjo, 1992). However, others have observed the occasional presence of B-lymphocytes in the human pulp (Hahn et al, 1989;Izumi et al, 1995).…”