Flow Cytometric Analysis of Peripheral Blood Lymphocyte Subsets in Patients with Silicone Breast Implants

Prantl, Lukas; Fichtner‐Feigl, Stefan; Hofstaedter, Ferdinand; Lenich, Andreas; Eisenmann‐Klein, Marita; Schreml, Stephan

doi:10.1097/01.prs.0000293758.61624.68

Cited by 16 publications

(8 citation statements)

References 36 publications

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“…We believe that bacteria were introduced during the injection procedure and formed a persistent and intractable infection on the glabella by forming a biofilm [7, 8, 9, 10]. Previous reports put forth that infection may occur late after an injection and may be related to immune responses [8, 9]. Although we did not examine whether the infiltrating T cells were effector T cells or regulatory T cells, persisting effector T cells can become exhausted and function inefficiently in situ [11].…”

Section: Discussionmentioning

confidence: 99%

A Case of Foreign-Body Granuloma of the Glabella due to Polyacrylamide Filler and an Intractable Ulcer after Skin Biopsy: An Immunohistochemical Evaluation of Inflammatory Changes

2013

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Introduction: Polyacrylamide hydrogel has been considered a safe and biocompatible soft tissue filler, and it has been widely used in cosmetic procedures. However, recent studies have revealed some complications with polyacrylamide filler injections. Case Report: We present the case of foreign-body granulomas of the glabella, which subsequently formed an infectious ulcer 3 years after a polyacrylamide injection. An immunohistochemical evaluation of the foreign-body granulomas was performed in order to study the relationship between foreign-body granulomas and immune response. Conclusion: We believe that our analysis of foreign-body granulomas 1 and 3 years after a filler injection may contribute to revealing the mechanism of chronic and intractable infections after filler injections.

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Section: Discussionmentioning

confidence: 99%

A Case of Foreign-Body Granuloma of the Glabella due to Polyacrylamide Filler and an Intractable Ulcer after Skin Biopsy: An Immunohistochemical Evaluation of Inflammatory Changes

2013

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“…These effects are mediated by indirect and direct lymphocyte interactions with monocytes, macrophages, and FBGCs, but we still do not have a full mechanistic understanding of these interactions. There is also evidence of lymphocyte activation from exposure to implanted prostheses such as silicone gel breast implants and left ventricular assist devices (LVAD) which can impact immune function 4–6. Patients with LVADs have shown an increased risk of infection as well as the presence of auto‐reactive antibodies 7.…”

Section: Introductionmentioning

confidence: 99%

Studies on α to β phase transformations in mechanically deformed PVDF films

Vijayakumar¹,

Khakhar²,

Misra³

2010

J of Applied Polymer Sci

104

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PVDF cast films were drawn at different temperatures to different draw ratios at constant draw rate to understand the mechanism of a to b phase transformation during mechanical deformation. WAXD and FTIR studies were carried out to determine the formation and content of b phase in the drawn films. Lower stretch temperatures gave higher fractions of b phase. The cast PVDF films were also drawn at suitable temperatures below the PVDF ambient melting point to the draw ratio of 6.4. The highest fraction of b phase obtained in these ultra drawn films was 0.98. SALS studies carried out for films at different stretch ratios show the change in spherulitic structure with the stretching parameters and give information for the understanding of phase transformation during stretching of PVDF films.

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Section: Introductionmentioning

confidence: 99%

“…There is also evidence of lymphocyte activation from exposure to implanted prostheses such as silicone gel breast implants and left ventricular assist devices (LVAD) which can impact immune function. [4][5][6] Patients with LVADs have shown an increased risk of infection as well as the presence of auto-reactive antibodies. 7 The elucidation of lymphocyte behavior and interactions are vital as we continue to develop novel biomaterials, devices, and other therapeutic technologies utilizing synthetic materials.…”

Section: Introductionmentioning

confidence: 99%

Lymphocyte adhesion and interactions with biomaterial adherent macrophages and foreign body giant cells

Chang

Colton

Matsuda

et al. 2009

J Biomedical Materials Res

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To characterize the effects of adherent macrophages and biomaterial surface chemistries on lymphocyte adhesion and activation, lymphocytes were co-cultured with monocytes alone and together, directly and separated by a porous membrane transwell on hydrophobic, hydrophilic/ neutral, hydrophilic/anionic, and hydrophilic/cationic biomaterial surfaces. Surface adherent cells were quantitatively analyzed after 3 days utilizing immunofluorescence and phase contrast imaging. After periods of 3, 7, and 10 days, secreted interferon-γ (IFN-γ) was quantified by ELISA. Limited direct biomaterial-adherent lymphocytes were identified regardless of the presence of macrophages or foreign body giant cells (FBGC). The majority of adherent lymphocytes, which were T cells (> 95%) rather than natural killer cells, predominantly interacted with adherent macrophages and FBGCs; greater than 90% were interacting on surfaces with higher levels of adherent macrophages and FBGCs and greater than 55% were interacting on surfaces with lower levels of macrophages and FBGCs. The hydrophilic/anionic surface promoted higher levels of macrophage-and FBGC-adherent lymphocytes but was nonselective for lymphocyte subtype interactions. The hydrophilic/neutral surface was selective for CD4+ T lymphocyte interactions while the hydrophobic surface was selective for CD8+ T lymphocyte interactions. IFN-γ was produced in direct and indirect co-cultures but not in lymphocyte-and monocyte-only cultures suggesting that lymphocytes are activated via macrophage-derived cytokines rather than direct biomaterial contact. Direct lymphocyte interactions with adherent macrophages/FBGCs enhanced IFN-γ production relative to indirect co-cultures. These results suggest that lymphocytes prefer interactions with adherent macrophages and FBGCs, resulting in lymphocyte activation, and these interactions can be influenced by biomaterial surface chemistries.

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Flow Cytometric Analysis of Peripheral Blood Lymphocyte Subsets in Patients with Silicone Breast Implants

Cited by 16 publications

References 36 publications

A Case of Foreign-Body Granuloma of the Glabella due to Polyacrylamide Filler and an Intractable Ulcer after Skin Biopsy: An Immunohistochemical Evaluation of Inflammatory Changes

A Case of Foreign-Body Granuloma of the Glabella due to Polyacrylamide Filler and an Intractable Ulcer after Skin Biopsy: An Immunohistochemical Evaluation of Inflammatory Changes

Studies on α to β phase transformations in mechanically deformed PVDF films

Lymphocyte adhesion and interactions with biomaterial adherent macrophages and foreign body giant cells

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