Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two  Turkish patients with Glanzmann thrombasthenia: a multicenter experience

Saraymen, Berkay; Muhtaroğlu, Sabahattin; Köker, Mustafa Yavuz; Sarper, Nazan; Zengin, Emine; Albayrak, Canan; Albayrak, Davut; Zülfikar, Bulent; Şenol, Başak Koç; Bentli, Esma; Yılmaz, Semih; Çetin, Aysun; Eser, Bülent; Çetın, Mustafa

doi:10.3906/sag-2006-107

Cited by 4 publications

(1 citation statement)

References 21 publications

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“…Flow cytometric analysis with GPs was used in the diagnosis of Glanzmann's thrombasthenia. Flow cytometry is the most sensitive method[ 27 ].…”

Section: F Low Cytometry For Assessing Markers Of Platelet ...mentioning

confidence: 99%

Flow cytometry for evaluating platelet immunophenotyping and function in patients with thrombocytopenia

Shen

2022

Tzu Chi Medical Journal

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Platelets play an essential role in primary hemostasis through bleeding and thromboembolism. Thus, the diagnosis or evaluation of impaired hereditary, acquired, and drug-related platelet dysfunction has become imperative. The assessment of the platelet function is too complex for routine platelet function study. The major methods involved in platelet function study include platelet function analyzer testing, thromboelastography, thromboelastometry, light transmission aggregometry, and flow cytometry. The current review article focuses on the methods with flow cytometry for immunophenotyping of platelet and evaluating platelet function for platelet disorders, especially in patients with thrombocytopenia. According to the consensus published by the International Society on Thrombosis and Haemostasis, for inherited and acquired platelet disorders, the two major measures by which flow cytometry determines platelet function are glycoprotein IIb/IIIa/P-selectin (CD62p) expression and percentage of leukocyte–platelet aggregates. Using flow cytometry to determine platelet function has several advantages, including good sensitivity to low platelet counts, small blood volume required, and the nonnecessity of centrifugation. However, flow cytometry has still many limitations and challenges, with standardization for routine laboratory testing also proving difficult. Although flow cytometry is available for multipurpose and sensitive study of platelet functions at the same time, the challenging analysis gradually increases and needs to be addressed before reality.

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“…Flow cytometric analysis with GPs was used in the diagnosis of Glanzmann's thrombasthenia. Flow cytometry is the most sensitive method[ 27 ].…”

Section: F Low Cytometry For Assessing Markers Of Platelet ...mentioning

confidence: 99%

Flow cytometry for evaluating platelet immunophenotyping and function in patients with thrombocytopenia

Shen

2022

Tzu Chi Medical Journal

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Flow cytometry immunophenotyping of healthy platelets and hospitalized patients with suspected platelet dysfunction: Challenges for establishing a cutoff value

Keller Cecconello,

Spagnol,

Alegretti

et al. 2024

Hematology, Transfusion and Cell Therapy

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Possibilities and limitations of platelet counting methods

Tsvetkova

Калашникова

2022

Medicinskij alfavit

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Currently, the use of hematological analyzers makes it possible to quickly and accurately count the number of platelets in the vast majority of blood samples. In some cases, the automatic determination of this indicator may be incorrect, due to the limitations of the methods used. Monitoring the results of automatic platelet count is necessary when obtaining pathological values and is carried out by microscopic examination of a peripheral blood smear. The article describes the methods and limitations of automatic determination of the number of platelets, possible causes of erroneous counting and ways to correct the result. Morphological descriptions of the most common artifacts that affect the correct definition of this indicator are presented. Modern methodological approaches to solving the problem of correct platelet counting in EDTA-dependent pseudothrombocytopenia are highlighted.

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Flow cytometric analysis of platelet surface glycoproteins in the diagnosis of thirty-two Turkish patients with Glanzmann thrombasthenia: a multicenter experience

Cited by 4 publications

References 21 publications

Flow cytometry for evaluating platelet immunophenotyping and function in patients with thrombocytopenia

Flow cytometry for evaluating platelet immunophenotyping and function in patients with thrombocytopenia

Flow cytometry immunophenotyping of healthy platelets and hospitalized patients with suspected platelet dysfunction: Challenges for establishing a cutoff value

Possibilities and limitations of platelet counting methods

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