Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes of Aotus nancymaae, a non-human primate model for biomedical research

Daubenberger, Claudia; Spirig, Rolf; Patarroyo, Manuel E.; Pluschke, Gerd

doi:10.1016/j.vetimm.2007.06.010

Cited by 10 publications

(5 citation statements)

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“…In some cases, adequate reactivity was observed, with variation in the intensity of staining depending on the clone and manufacturer used, although in many cases no reactivity was detected. Similarly, one study tested 204 antibodies against various human CD markers, and only 28% of these molecules reacted with Aotus splenocytes [ 51 ]; approximately 40% of the reacting antibodies also recognized rhesus and cynomolgus cells. The use of NHP CBA only facilitated the detection of IL-2, reflecting the broader specificity of antibodies to human cytokines that also cross-react with rhesus, cynomolgus, baboon and pig-tailed macaque cytokines.…”

Section: Discussionmentioning

confidence: 99%

Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research

Riccio

Pratt-Riccio

Bianco‐Júnior

et al. 2015

Malar J

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BackgroundThe neotropical, non-human primates (NHP) of the genus Saimiri and Aotus are recommended by the World Health Organization as experimental models for the study of human malaria because these animals can be infected with the same Plasmodium that cause malaria in humans. However, one limitation is the lack of immunological tools to assess the immune response in these models. The present study focuses on the development and comparative use of molecular and immunological methods to evaluate the cellular immune response in Saimiri sciureus.MethodsBlood samples were obtained from nineteen uninfected Saimiri. Peripheral blood mononuclear cells (PBMC) from these animals and splenocytes from one splenectomized animal were cultured for 6, 12, 18, 24, 48, 72 and 96 hrs in the presence of phorbol-12-myristate-13-acetate and ionomycin. The cytokine levels in the supernatant were detected using human and NHP cytometric bead array Th1/Th2 cytokine kits, the Bio-Plex Pro Human Cytokine Th1/Th2 Assay, enzyme-linked immunosorbent assay, enzyme-linked immunospot assays and intracellular cytokine secretion assays. Cytokine gene expression was examined through TaqMan® Gene Expression Real-Time PCR using predesigned human gene-specific primers and probes or primers and probes designed based on published S. sciureus cytokine sequences.ResultsThe use of five assays based on monoclonal antibodies specific for human cytokines facilitated the detection of IL-2, IL-4 and/or IFN-γ. TaqMan array plates facilitated the detection of 12 of the 28 cytokines assayed. However, only seven cytokines (IL-1A, IL-2, IL-10, IL-12B, IL-17, IFN-β, and TNF) presented relative expression levels of at least 70% of the gene expression observed in human PBMC. The use of primers and probes specific for S. sciureus cytokines facilitated the detection of transcripts that showed relative expression below the threshold of 70%. The most efficient evaluation of cytokine gene expression, in PBMC and splenocytes, was observed after 6–12 hrs of culture, except for LTA in PBMC, whose expression was best analysed after 24 hrs of culture.ConclusionsReal-time PCR facilitates the analysis of a large number of cytokines altered during malaria infection, and this technique is considered the best tool for the evaluation of the cellular immune response in S. sciureus.

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Section: Discussionmentioning

confidence: 99%

Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research

Riccio

Pratt-Riccio

Bianco‐Júnior

et al. 2015

Malar J

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“…Indeed, scientific publications resulting from research conducted by FIDIC indicate that A. nancymaae and A. nigriceps have been used by them. All these publications have been reported to Corpoamazonia [e.g., Cardenas et al, 2005;Curtidor et al, 2007;Daubenberger et al, 2007;Patarroyo et al, 2006;Rojas-Caraballo et al, 2009;Suárez et al, 2011]. Ruiz-Garcia et al [2013] conducted a molecular genetics analysis of mtDNA COII gene sequences reporting genetic evidence of the illegal trade conducted by FIDIC.…”

Section: Discussionmentioning

confidence: 99%

Research and in situ conservation of owl monkeys enhances environmental law enforcement at the Colombian‐Peruvian border

Maldonado¹,

Peck

2014

American J Primatol

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This study reports on impacts of illegal trade in owl monkeys (Aotus nancymaae, A. vociferans) for the biomedical research market in the Colombian‐Peruvian Amazonian border. Through freedom of information requests and interviews with hunters we found that 912 owl monkeys, including A. nancymaae captured in Peru, were trapped over a 3‐month period in 2012 to supply a malaria research facility based in Leticia, Colombia, which had trapping permits for the use of only 800 A. vociferans annually yet experimentation took place using A. nancymaae. High levels of extraction in Peru have had population‐level impacts with significantly lower densities of Aotus spp. (3–24 individuals/km2) compared to Colombian sites with low hunting pressure (26–44 individuals/km2). Post‐experimental release of this species in Colombian territory has created a new distribution whose status and impacts on resident populations of A. vociferans remain unknown. The trapping method has also had environmental impact, with loss of over 65,000 trees (including sleeping sites), annually. As Aotus species are registered under the Convention of International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II, international trade requires official permission and evidence that extraction does not impact wild populations. However, no official records exist and CITES legislation has failed, due principally to a lack of appropriate monitoring by national authorities responsible for compliance. Of further concern is that we had previously documented and reported the illegal trade to the appropriate governmental authorities yet still no action was taken—as demonstrated by the continuing trade in 2013. Enforcement eventually occurred when a non‐governmental organization initiated legal action against organizations responsible. A successful second instance ruling by the Colombian State's Council in 2013 revoked trapping permits. Using the trade in owl monkeys as a case study we consider implementation, compliance, and enforcement of CITES in the border area to identify mechanisms to improve enforcement of environmental legislation. Am. J. Primatol. 76:658–669, 2014. © 2014 Wiley Periodicals, Inc.

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“…Publications resulting from research conducted at the facility in the Amazonas Department indicate that indeed both A. nancymaae and A. nigriceps are used (e.g. Cardenas et al 2005, Spirig et al 2005, Suarez et al 2006, Daubenberger et al 2007.…”

Section: Discussionmentioning

confidence: 99%

Trade in night monkeys Aotus spp. in the Brazil–Colombia–Peru tri-border area: international wildlife trade regulations are ineffectively enforced

Maldonado¹,

Nijman²,

Bearder³

2009

Endang. Species. Res.

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This study describes significant levels of trade in 2 or possibly 3 species of night monkeys (Aotus nancymaae, A. vociferans and A. nigriceps) from the Brazil-Colombia-Peru tri-border area. All 3 countries are Party to the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) and there is no documented trade in night monkeys among these 3 countries in the CITES trade database. However, interviews with 43 traders/collectors in 11 communities in the 3 countries suggest that for the period 2007-2008, ca. 4000 night monkeys were traded, representing a monetary value of over USD 100 000 for the traders and intermediaries. The interviewees indicated that the animals were sold to a biomedical laboratory in the tri-border area on the Colombian side of the border. The international nature of the trade and the large volume of night monkeys being traded indicate a violation of, and a failure to adhere to, international trade regulations. In order to conserve these important species, we suggest cooperative action from environmental and conservation authorities and the respective CITES Management Authorities in Colombia, Peru and Brazil to curb the trade, and urge the Colombian authorities to investigate the illegal importation of night monkeys by a biomedical laboratory in the border area. KEY WORDS: CITES · Primate conservation · Amazonia · Biomedical research Resale or republication not permitted without written consent of the publisher Contribution to the Theme Section 'Primate conservation: measuring and mitigating trade in primates'OPEN PEN

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Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes of Aotus nancymaae, a non-human primate model for biomedical research

Cited by 10 publications

References 23 publications

Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research

Molecular and immunological tools for the evaluation of the cellular immune response in the neotropical monkey Saimiri sciureus, a non-human primate model for malaria research

Research and in situ conservation of owl monkeys enhances environmental law enforcement at the Colombian‐Peruvian border

Trade in night monkeys Aotus spp. in the Brazil–Colombia–Peru tri-border area: international wildlife trade regulations are ineffectively enforced

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