Flow Cytometric Determination of Nuclear Replication Stages in Tomato Seeds during Priming and Germination

Bino, R.J.; Vries, J. N. de; Kraak, H.L.; Pijlen, J.G. van

doi:10.1093/oxfordjournals.aob.a088335

Cited by 120 publications

(85 citation statements)

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“…Flow cytometry was performed according to a protocol modified from Bino et al (1992), and Arumuganathan and Earle (1991) on a BD Biosciences FACSCalibur flow cytometer (BD Biosciences, San Jose, CA, USA), using three to five young leaves per plant (approx. 15-20 mm in length, resulting in approximately 70 mg of tissue) stained with propidium iodide.…”

Section: Dna Contentmentioning

confidence: 99%

Inheritance and dominance of self-incompatibility alleles in polyploid Arabidopsis lyrata

2004

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Natural populations of diploid Arabidopsis lyrata exhibit the sporophytic type of self-incompatibility system characteristic of Brassicaceae, in which complicated dominance interactions among alleles in the diploid parent determine selfrecognition phenotypes of both pollen and stigma. The purpose of this study was to investigate how polyploidy affects this already complex system. One tetraploid population (Arabidopsis lyrata ssp kawasakiana from Japan) showed complete self-compatibility and produced viable selfed progeny for at least three generations subsequent to field collection. In contrast, individuals from a second tetraploid population (A. lyrata ssp petraea from Austria) were strongly self-incompatible (SI). Segregation of SI genotypes in this population followed Mendelian patterns based on a tetrasomic model of inheritance, with two to four alleles per individual, independent segregation of alleles, and little evidence of dosage effects of alleles found in multiple copies. Similar to results from diploids, anomalous compatibility patterns involving particular combinations of individuals occurred at a low frequency in the tetraploids, suggesting altered dominance in certain genetic backgrounds that could be due to the influence of a modifier locus. Overall, dominance relationships among S-alleles in self-incompatible tetraploid families were remarkably similar to those in related diploids, suggesting that this very important and complicated locus has not undergone extensive modification subsequent to polyploidization.

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Section: Dna Contentmentioning

confidence: 99%

Inheritance and dominance of self-incompatibility alleles in polyploid Arabidopsis lyrata

2004

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“…Osmotic priming was carried out on top of filter paper soaked with 30% (w/v) PEG-6000 (-1.0 MPa) in a sealed Petri dish from 6 h to a maximum of 7 d at 20°C in darkness (Bino et al, 1992). After priming, seeds were washed for 5 min under running tap water to remove PEG from the seed coat.…”

Section: Priming Aging and Lmbibition Conditionsmentioning

confidence: 99%

“…and pepper (Capsicurn annuum L.), imbibition is coupled to initiation of DNA replication in cells of the embryo root tip, as was demonstrated by flow cytometric analysis of isolated nuclei (Bino et al, 1992;Lanteri et al, 1993). In these species, the embryonic cells progress through the S phase of the cell cycle into the G, phase before visible germination.…”

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confidence: 98%

“…In imbibing tomato seeds, the cell cycle progresses to G, prior to germination (Bino et al, 1992). This accumulation of cells in G, takes place in tomato because mitosis and cell division do not finish prior to protrusion of the root tip through the seed coat (Argerich and Bradford, 1989).…”

mentioning

confidence: 99%

“…This accumulation of cells in G, takes place in tomato because mitosis and cell division do not finish prior to protrusion of the root tip through the seed coat (Argerich and Bradford, 1989). Moreover, by preconditioning seeds in an osmotic solution (priming) followed by redrying, tomato seeds can be stably arrested in G, (Bino et al, 1992). Priming may improve both the rate and the uniformity of seed germination upon subsequent imbibition of water (Heydecker and Coolbear, 1977).…”

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confidence: 99%

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[beta]-Tubulin Accumulation and DNA Replication in Imbibing Tomato Seeds

et al. 1995

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The activation of the cell cycle in embryo root tips of imbibing tomato (Lycopersicon esculentum Mill. cv Lerica) seeds was studied by flow cytometric analyses of the nuclear DNA content and by immunodetection of p-tubulin. With dry seeds, flow cytometric profiles indicated that the majority of the cells were arrested at the C , phase of the cell cycle. In addition, p-tubulin was not detectable on western blots. Upon imbibition of water, the number of cells in C , started to increase after 24 h, and a 55-kD fl-tubulin signal was detected between 24 and 48 h. Two-dimensional immunoblots revealed at least three different p-tubulin isotypes. Thus, p-tubulin accumulation and DNA replication were induced during osmotic priming. These processes, as well as seed germination rate, were enhanced upon subsequent imbibition of water, compared with control seeds that imbibed but were not primed. By contrast, when aged seeds imbibed, DNA replication, P-tubulin accumulation, and germination were delayed. In all cases studied, both DNA replication and p-tubulin accumulation preceded visible germination. We suggest that activation of these cell-cycle-related processes is a prerequisite for tomato seed germination. Furthermore, p-tubulin expression can be used as a parameter for following the initial processes that are activated during seed imbibition.

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