Flow cytometry and immune thrombocytopenic purpura

Goubran, Hadi; Hart, Caroline; Othman, I.; Seghatchian, Jerard

doi:10.1016/j.transci.2018.10.018

Cited by 8 publications

(6 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A patogênese da PTI não é completamente esclarecida, sendo relacionada a uma destruição de plaquetas e inibição da sua produção. A destruição das plaquetas ocorre pelos macrófagos presentes no baço (sistema monuclear fagocitário) e é determinada pela produção de IgG pelos linfócitos B. Múltiplos antígenos na superfície plaquetária parecem ser responsáveis pela ligação com anticorpos, especialmente a glicoproteína IIb/ IIIa e a Ib/IX, que quando ligados desencadeiam o processo de destruição de plaquetas (CHONG, 2009;GOUBRAN et al, 2018). KRETTLI (2011) demonstrou que os autoanticorpos que promovem a destruição das plaquetas estão presentes em 50 a 70% dos pacientes, o que indica outro mecanismo de destruição a ser investigado.…”

Section: Resultsunclassified

“…Dados de estudos epidemiológicos internacionais em adultos, que fornecem uma estimativa de incidência de 1,6-2,7 casos por 100.000 pessoas/ano e uma prevalência de 9,5-23,6 casos por 100.000 pessoas, com predominância no sexo feminino (ABRAHAMSON et al, 2009), havendo um leve discordância com o estudo de Kurtzberg (1992). No estudo de Goubran (2018) a incidência deste distúrbio é de cerca de 2 e 12/100.000 por ano para adultos e crianças, respectivamente, com mortalidade potencial em alguns casos. A PTI aguda, ocorrendo principalmente em crianças, tem um início e resolução em menos de 6 meses.…”

Section: Introductionunclassified

See 1 more Smart Citation

Púrpura trombocitopênica idiopática: uma doença subdiagnosticada

Alves

Silva

et al. 2021

Sustinere

View full text Add to dashboard Cite

Introdução: A púrpura trombocitopênica idiopática (PTI) é caracterizada pela destruição de plaquetas e inibição da sua produção, que se evidencia por trombocitopenia isolada. Trata-se de uma doença autoimune subdiagnosticada, pois não existe um exame próprio para diagnostica-la e devido tal circunstância a PTI é confundida com outras doenças que causam diminuição de plaquetas. Esta revisão objetiva retratar as características da púrpura trombocitopênica idiopática, como é feito seu diagnóstico e o seu tratamento. Metodologia: Para a construção desta revisão integrativa, realizou-se uma busca eletrônica de artigos de periodicidade, na Biblioteca Virtual de Saúde (BVS), nas bases de dados do Lilacs, Medline e Scielo (Scientific Electronic Library Online). Foram utilizados artigos escritos nas línguas portuguesa, inglesa e espanhola; publicados e indexados entre os anos 2000 e 2019 nas bases de dados escolhidas, que respondiam um dos seguintes questionamentos: quais os métodos utilizados para o diagnóstico de PTI e como realizar o tratamento nas diferentes etapas da doença? Resultados: A patogênese da PTI não é completamente esclarecida, sendo relacionada a uma destruição de plaquetas e inibição da sua produção. Discussão: O tratamento pode ser realizado das seguintes formas: tratamento inicial, tratamento de segunda linha, tratamento da doença refratária e tratamento em situações de emergência. Conclusão: A PTI trata-se de uma doença autoimune subdiagnosticada, pois não existe um exame próprio para diagnosticar essa patologia, devido tal circunstância a PTI é confundida com outras doenças que causam diminuição de plaquetas.

show abstract

Section: Resultsunclassified

Section: Introductionunclassified

Púrpura trombocitopênica idiopática: uma doença subdiagnosticada

Alves

Silva

et al. 2021

Sustinere

View full text Add to dashboard Cite

show abstract

“…Immune thrombocytopenic purpura is de ned as an acquired autoimmune disorder characterized by immunemediated platelet destruction due to the binding of immunoglobulin IgG autoantibodies with platelet glycoproteins [27].…”

Section: Discussionmentioning

confidence: 99%

Study the Incidence of TNF-α-induced Protein 3 Genetic Polymorphisms in Primary Immune Thrombocytopenia Patients

Zanaty

Korayem

Meabed

et al. 2021

Preprint

View full text Add to dashboard Cite

BackgroundImmune Thrombocytopenia (ITP) is a relatively common acquired hematological disorder, affecting 2 to 4/100000 adults. Understanding of the pathogenesis of ITP has been greatly improved with taking into consideration the important role of the genetic variants. This study aimed at investigating the incidence of TNFAIP3 SNPs (rs2230926 and rs5029939) in primary ITP Egyptian patients as well as their response to therapy in addition to the linkage between the two SNPs.Methods and Resultsthe study was conducted in 110 ITP patients diagnosed as primary ITP (PITP) selected among cases referred to the Hematology Outpatient Clinic of Kasr El Aini Hospital and 110 matched healthy controls. The polymorphisms were detected by real-time polymerase chain reaction (real-time PCR). Data indicated that there is a significant difference in the allelic distribution between PITP patients and the control group regarding rs2230926 and rs5029939 (p-value <0.05). Regarding LD analysis of the two SNPs, it has been revealed that there was a significant linkage disequilibrium between rs2230926 and rs5029939 among PITP group LD (D' = 0.966, r2 = 0.694, p-value < 0.001). On behalf of improvement by treatment, patients with rs2230926 wild genotype showed a more significant response to treatment than mutant type (p-value <0.05).ConclusionThere was a correlation between TNFAIP3 SNPs (rs2230926 and rs5029939) and the occurrence of primary ITP in the adult Egyptian populations and there was a linkage disequilibrium between them. Moreover, patients with rs2230926 wild genotype showed significant improvement than mutant type.

show abstract

“…This cross-sectional study was conducted at the Armed Forces Institute of Pathology from June 2021 to December 2021, over seven months after permission from the Institutional Review Board was taken (IRB no FC-HEM 20-21/READ-IRB/22/840). Keeping the confidence level at 95%, the margin of error at 5% and taking the population proportion to 95% based on a study conducted on NIBD, 11 in which the majority of patients with ITP had raised IPF. The WHO calculator was used to calculate the sample size of 73.…”

Section: Methodsmentioning

confidence: 99%

“…10 Bone marrow biopsy is a procedure which can cause pain; therefore, the flow cytometric technique is now being utilized to reach the final diagnosis. 11 In the present era, IPF can be used to differentiate the cases of ITP and central thrombocytopenia. Studies have shown that under circumstances of thrombocytopenia, the RNA content of platelets corresponds directly with megakaryopoiesis.…”

Section: Immature Platelet Fraction (Ipf%) In Diagnosed Patientsmentioning

confidence: 99%

Significance of Immature Platelet Fraction in Diagnosed Patients of the Immune Thrombocytopenic Purpura

Haider

Mahmood

Bozdar

et al. 2022

PAFMJ

View full text Add to dashboard Cite

Objective: Determination of significance of immature platelet fraction (IPF%) in diagnosed patients of Immune thrombocytopenic purpura (ITP). Study Design: It was a cross-sectional study. Place and Study Duration: Haematology department, Armed forces Institute of Pathology (AFIP), Rawalpindi Pakistan June 2021 to December 2021. Methodology: Patients of all ages and genders diagnosed with ITP and platelets less than 100 x 109/L after a review of peripheral blood film were included in the study. Detailed history and examination were made, complete blood count (CBC) test was done on Sysmex XN-3000 automated haematology analyzer. Peripheral blood was stained with Leishman stain and examined under a microscope to rule out pseudo thrombocytopenia and the presence of abnormal cells and dysplasia. After adequate quality and control, the immature platelet fraction was performed on Sysmex XN 3000. Results: Seventy-two diagnosed cases of immune thrombocytopenic purpura were part of the study. The mean age of the patients was 21.00±14.50 years. 34 (48%) patients were males, and 38 (52%) were females. The mean platelet count was 49.40±24.60. Mean IPF was 17.90±9.50 per microliter. Immature platelet fraction (IPF%) was raised in all the patients with immune thrombocytopenic purpura, confirming our hypothesis that IPF% was an independent predictor for the detection of ITP. Conclusion: Immature platelet fraction is a unique parameter that can pick patients having thrombocytopenia due to peripheral destruction, including immune thrombocytopenic purpura.

show abstract

Flow cytometry and immune thrombocytopenic purpura

Cited by 8 publications

References 33 publications

Púrpura trombocitopênica idiopática: uma doença subdiagnosticada

Púrpura trombocitopênica idiopática: uma doença subdiagnosticada

Study the Incidence of TNF-α-induced Protein 3 Genetic Polymorphisms in Primary Immune Thrombocytopenia Patients

Significance of Immature Platelet Fraction in Diagnosed Patients of the Immune Thrombocytopenic Purpura

Contact Info

Product

Resources

About