Objective: To categorize glucose-6-phosphate dehydrogenase (G6PD) deficiency based on enzyme activity and its clinical haematological correlation. Methodology: This Cross-sectional study was conducted at the Department of Haematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi, from February 2022 to August 2022. Sampling was done using the nonprobability consecutive sampling technique. Test analysis included a complete blood picture, RBC morphology and reticulocyte count, G6PD quantitative test, and serum bilirubin. Thus, to categorize G6PDD based on its enzyme and clinic-haematological correlation, study included patients of both gender with an age ranging from 0-76 years. Descriptive statistics were expressed as mean ± standard deviation (SD) and categorical data were presented as frequency and percentage. Results: Out of 120 study participants, 30 (25%) were females and 90 (75%) were males. The mean age of study participants was 10.83±12.75. G6PD PCR was detected among participants having G6PD deficiency level <1 U/g Hb and between 2-3 U/gm Hb. Hb levels below 8g/dL were found only in individuals with G6PD deficiency levels <1 U/gm Hb. Conclusion: GDPD deficiency can be diagnosed by blood analysis comprising of complete blood count and RBC morphology aided by clinical correlation. The signs and symptoms increase in severity with a decline in GDPD enzyme function along with blood haemoglobin levels.

Objective: Determination of significance of immature platelet fraction (IPF%) in diagnosed patients of Immune thrombocytopenic purpura (ITP). Study Design: It was a cross-sectional study. Place and Study Duration: Haematology department, Armed forces Institute of Pathology (AFIP), Rawalpindi Pakistan June 2021 to December 2021. Methodology: Patients of all ages and genders diagnosed with ITP and platelets less than 100 x 109/L after a review of peripheral blood film were included in the study. Detailed history and examination were made, complete blood count (CBC) test was done on Sysmex XN-3000 automated haematology analyzer. Peripheral blood was stained with Leishman stain and examined under a microscope to rule out pseudo thrombocytopenia and the presence of abnormal cells and dysplasia. After adequate quality and control, the immature platelet fraction was performed on Sysmex XN 3000. Results: Seventy-two diagnosed cases of immune thrombocytopenic purpura were part of the study. The mean age of the patients was 21.00±14.50 years. 34 (48%) patients were males, and 38 (52%) were females. The mean platelet count was 49.40±24.60. Mean IPF was 17.90±9.50 per microliter. Immature platelet fraction (IPF%) was raised in all the patients with immune thrombocytopenic purpura, confirming our hypothesis that IPF% was an independent predictor for the detection of ITP. Conclusion: Immature platelet fraction is a unique parameter that can pick patients having thrombocytopenia due to peripheral destruction, including immune thrombocytopenic purpura.