Flow cytometry: basic principles and applications

Adan, Aysun; Alizada, Günel; Kiraz, Yağmur; Baran, Yusuf; Nalbant, Ayten

doi:10.3109/07388551.2015.1128876

Cited by 692 publications

(518 citation statements)

References 28 publications

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“…FC gives a statistically relevant and quantitative signal of fluorescence very quickly and allows sensitive detection. The technique provides both qualitative and quantitative information [159,250,252].…”

Section: Live Imagingmentioning

confidence: 99%

Membrane Active Peptides and Their Biophysical Characterization

Avcı

Akbulut

Özkırımlı

2018

Preprint

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In the last 20 years, an increasing number of studies have been reported on membrane active peptides, which exert their biological activity by interacting with the cell membrane either to disrupt it and lead to cell lysis or to translocate through it to deliver cargos into the cell and reach their target. These peptides are attractive alternatives to currently used pharmaceuticals. Antimicrobial peptides (AMPs) and peptides designed for drug and gene delivery currently in the drug pipeline suggest that these membrane active peptides will soon constitute a significant percentage of the drug market. Here, we focus on two most prominent classes of membrane active peptides; AMPs and cell-penetrating peptides (CPPs). AMPs are a group of membrane active peptides that disrupt the membrane integrity or inhibit the cellular functions of bacteria, virus and fungi. CPPs are another group of membrane active peptides that mainly function as cargo-carriers even though they may also show antimicrobial activity to some extent. Biophysical techniques to understand how they interact with the membrane have shed light on the peptide-membrane interaction at various levels of detail. Structural investigation of membrane active peptides in the presence of the membrane provides important clues on the effect of the membrane environment on peptide conformations. Advances in live imaging techniques have allowed examination of peptide action at a single cell or single molecule level. In addition to these experimental biophysical techniques, molecular dynamics simulations provided clues on the peptide-lipid interactions and dynamics of the cell entry process at atomic detail. In this review, we summarize the recent advances in experimental and computational investigation of membrane active peptides with particular emphasis on two amphipathic membrane active peptides, the AMP melittin and the CPP pVEC.

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Section: Live Imagingmentioning

confidence: 99%

Membrane Active Peptides and Their Biophysical Characterization

Avcı

Akbulut

Özkırımlı

2018

Preprint

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“…Complex biosafety systems must be employed to reduce the potential of infection for the operator. [21,22] …”

Section: Flow Cytometry (Fcm)mentioning

confidence: 99%

Diagnostic aids in detection of oral precancer and cancer: Past to present

Nambiar¹,

Haragannavar²,

Augustine³

et al. 2016

IDJAR

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Oral carcinogenesis is a stepwise accumulation of genetic damage over time. The older cancer diagnostic aids had less specificity, were time-consuming, and produced interobserver bias. Technological and therapeutic advances in the recent years have helped to diagnose and treat this disease at an early stage. Advances in molecular biology over the past decade have helped us to enhance our understanding of the complex interplay between genetic, transcriptional, and translational alterations in human cancers. This review provides a summary of all the diagnostic modalities that were used earlier and the newer more advanced techniques with merits and demerits of each technique described briefly.

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“…Among the possible physical properties which enable distinguishing cancer cells from healthy cells, the response of a cell to electromagnetic (EM) waves in principle enables fast detection and separation even in the case of extremely small numbers like CTCs. Optical scattering represents the most commonly used method to determine cell size and cell inhomogeneity by scattering within standard flow cytometry [3]. There is a natural demand to utilize lower frequency bands, and the maturing of microfluidic technology encourages the development and exploration of integrated microfluidic devices with EM detection capability over a wider range of frequencies.…”

Section: Introductionmentioning

confidence: 99%

Microwave-to-terahertz dielectric resonators for liquid sensing in microfluidic systems

et al. 2016

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The microwave-to-terahertz frequency range offers unique opportunities for the sensing of liquids based on the degree of molecular orientational and electronic polarization, Debye relaxation due to intermolecular forces between (semi-)polar molecules and collective vibrational modes within complex molecules. Methods for the fast dielectric characterization of (sub-)nanolitre volumes of mostly aqueous liquids and biological cell suspensions are discussed, with emphasis on labon-chip approaches aimed towards single-cell detection and label-free flow cytometry at microwave-to-terahertz frequencies. Among the most promising approaches, photonic crystal defect cavities made from high-resistivity silicon are compared with metallic split-ring resonant systems and high quality factor (Q-factor) whispering gallery-type resonances in dielectric resonators. Applications range from accurate haemoglobin measurements on nanolitre samples to label-free detection of circulating tumor cells.

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Flow cytometry: basic principles and applications

Cited by 692 publications

References 28 publications

Membrane Active Peptides and Their Biophysical Characterization

Membrane Active Peptides and Their Biophysical Characterization

Diagnostic aids in detection of oral precancer and cancer: Past to present

Microwave-to-terahertz dielectric resonators for liquid sensing in microfluidic systems

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