Flow cytometry of human semen: a preliminary study of a non-invasive method for the detection of spermatogenetic defects

Abstract

The method described may serve as a simple, non-invasive and reliable assay to help clinicians counsel patients with severe male infertility before referring them for testicular surgery to locate spermatozoa for ICSI.

Assessment of sperm chromatin condensation and ploidy status using flow cytometry correlates to fertilization, embryo quality and pregnancy following in vitro fertilization

“…The methods of AO and PI stainings in combination with SFC were used to study the abnormal chromatin condensation and the ploidy of human spermatozoa, respectively, according to protocols previously described [25,26]. Briefly, two semen samples from each patient were used for the analysis, so as to ensure reliable results.…”

Using semen flow cytometry to evaluate association of ploidy status and chromatin condensation of spermatozoa with conventional semen parameters: Clinical application in intrauterine insemination

“…The method of propidium iodide staining and SFC was used to study the ploidy status of human spermatozoa, according to a protocol described previously (18). Two semen samples from each patient were used for the analysis to ensure reliable results.…”

Association of sperm apoptosis and DNA ploidy with sperm chromatin quality in human spermatozoa

“…Therefore, by using DNA cytometry, we can differentiate aneuploidy, triploidy, or tetraploidy from diploidy (40,41) and even differentiate various maturation stages of spermatozoa (42)(43)(44)(45). Essentially, these are the haploid sperm with different levels of differentiation (representing the immature haploid and mature condensed spermatozoa) and loss of DNA content (representing the subhaploid sperm).…”