Flow-free transport of cells in microchannels by frequency-modulated ultrasound

Manneberg, Otto; Vanherberghen, Bruno; Önfelt, Björn; Wiklund, Martin

doi:10.1039/b816675g

Cited by 102 publications

(76 citation statements)

References 15 publications

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“…To date, many acoustic-based particle manipulation functions (e.g., focusing, separating, sorting, mixing, and patterning) have been realized (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42)(43). None of these approaches, however, have achieved the dexterity of optical tweezers; in other words, none of the previous acoustic-based methods are capable of precisely manipulating single microparticles or cells along an arbitrary path in two dimensions.…”

mentioning

confidence: 99%

On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves

Ding

Lin

Kiraly

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

799

590

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Techniques that can dexterously manipulate single particles, cells, and organisms are invaluable for many applications in biology, chemistry, engineering, and physics. Here, we demonstrate standing surface acoustic wave based “acoustic tweezers” that can trap and manipulate single microparticles, cells, and entire organisms (i.e., Caenorhabditis elegans ) in a single-layer microfluidic chip. Our acoustic tweezers utilize the wide resonance band of chirped interdigital transducers to achieve real-time control of a standing surface acoustic wave field, which enables flexible manipulation of most known microparticles. The power density required by our acoustic device is significantly lower than its optical counterparts (10,000,000 times less than optical tweezers and 100 times less than optoelectronic tweezers), which renders the technique more biocompatible and amenable to miniaturization. Cell-viability tests were conducted to verify the tweezers’ compatibility with biological objects. With its advantages in biocompatibility, miniaturization, and versatility, the acoustic tweezers presented here will become a powerful tool for many disciplines of science and engineering.

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mentioning

confidence: 99%

On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves

Ding

Lin

Kiraly

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

799

590

View full text Add to dashboard Cite

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“…This provides a basis for particle-free optical resonance tracking. In principle, the optical tracking could be done for various frequencies simultaneously, which is of interest for multifrequency acoustophoresis experiments [18,46].…”

Section: Discussionmentioning

confidence: 99%

“…This opens up the possibility of in situ optical characterization of acoustic modes on the microscale, which is important for a variety of systems such as those used in shaped acoustic fields [11], acoustophoresis [46], and even piezoprintheads. Due to the spatial selectivity, the local pressure around micro-objects can be measured and novel configurations in acoustic microfluidics can be investigated.…”

Section: Discussionmentioning

confidence: 99%

Imaging local acoustic pressure in microchannels

et al. 2015

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A method for determining the spatially resolved acoustic field inside a water-filled microchannel is presented. The acoustic field, both amplitude and phase, is determined by measuring the change of the index of refraction of the water due to local pressure using stroboscopic illumination. Pressure distributions are measured for the fundamental pressure resonance in the water and two higher harmonic modes. By combining measurement at a range of excitation frequencies, a frequency map of modes is made, from which the spectral line width and Q-factor of individual resonances can be obtained.

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“…However the averaging effect is not perfect, particularly for lower flow rates. Frequency sweeping can also 55 reduce these effects by effectively averaging in time the force-fields of similar acoustic modes 32 .…”

Section: Modelling Of Planar Resonatorsmentioning

confidence: 99%

Acoustofluidics 9: Modelling and applications of planar resonant devices for acoustic particle manipulation

2012

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5This article introduces the design, construction and applications of planar resonant devices for particle and cell manipulation. These systems rely on the pistonic action of a piezoelectric layer to generate a one dimensional axial variation in acoustic pressure through a system of acoustically tuned layers. The resulting acoustic standing wave is dominated by planar variations in pressure causing particles to migrate to planar pressure nodes (or antinodes depending on particle and fluid properties). The consequences of 10 lateral variations in the fields are discussed, and rules for designing resonators with high energy density within the appropriate layer for a given drive voltage presented.  IntroductionThere is a need to manipulate micron-scale particles and cells in many areas of physics, analytical chemistry and the biosciences. Techniques such as filtration, centrifugation and sedimentation are well-established in macro-scale applications, but in microfluidic 15 systems the use of other approaches including optical, magnetic, dielectrophoretic and acoustic forces are of interest. Acoustic radiation forces, typically at ultrasonic frequencies in the hundreds of kHz to tens of MHz region, have wavelengths that are well matched to microfluidic channel scales, yet are capable of generating potential wells with significantly larger length scales. The technology is also relatively straightforward to integrate within microfluidic systems. Acoustic radiation forces 20Acoustic radiation forces can be generated on particles by both travelling and standing acoustic fields. Those in standing wave fields (of primary interest in the applications considered here) are generated by the nonlinear interaction between the acoustic field scattered by the particle and the standing wave field itself. The time averaged radiation force () F r on a small (in comparison with a wavelength) spherical particle of volume V located at r within a stationary acoustic field was shown by Gor'kov 1 to relate to the gradients of the time averaged kinetic and potential energy densities ( kin E and pot E respectively) within the field:The kinetic energy density gradient (a function of the acoustic velocity field within the standing wave) is weighted by a function of the densities ( on them that tends to move them to the acoustic pressure node, and the acoustic velocity antinode. In a planar resonator these are colocated. Generating the required acoustic fieldMany approaches to generating the required acoustic wave field have been reported in the literature. These include the use of near-field effects 2 or focussed ultrasound 3, 4 to trap particles and cells, the excitation of lateral standing waves in which the predominant energy 35 gradients run parallel to the face of the excitation transducer 5,6 , and the generation of cylindrical resonances to focus 7 or arrange

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Flow-free transport of cells in microchannels by frequency-modulated ultrasound

Cited by 102 publications

References 15 publications

On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves

On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves

Imaging local acoustic pressure in microchannels

Acoustofluidics 9: Modelling and applications of planar resonant devices for acoustic particle manipulation

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