Flow Injection Analysis System Using Magnetic Beads, Screen Printed Electrodes and Magnets

Biscay, Julien; García, María Begoña González; Garcı́a, Agustı́n Costa

doi:10.1002/elan.201400212

Cited by 3 publications

(4 citation statements)

References 25 publications

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“…397 Similarly to the above-mentioned applications, magnetic beads functionalized with streptavidin and accumulated temporarily on the surface of screen-printed working electrode, can be used in amperometric determination of biotin in FIA system and enzymatic marker. 398 An entirely different approach was reported for ultrasensitive amperometric ion detection with 5 nM LOD, which is based on the use of a supported interface between immiscible electrolyte solutions. 399 In the employed flow-through cell (Fig.…”

Section: Electrochemical Detectionsmentioning

confidence: 99%

Recent advances in flow injection analysis

Trojanowicz

Kołacińska

2016

Analyst

170

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A dynamic development of methodologies of analytical flow injection measurements during four decades since their invention has reinforced the solid position of flow analysis in the arsenal of techniques and instrumentation of contemporary chemical analysis. With the number of published scientific papers exceeding 20,000, and advanced instrumentation available for environmental, food, and pharmaceutical analysis, flow analysis is well established as an extremely vital field of modern flow chemistry, which is developed simultaneously with methods of chemical synthesis carried out under flow conditions. This review work is based on almost 300 original papers published mostly in the last decade, with special emphasis put on presenting novel achievements from the most recent 2-3 years in order to indicate current development trends of this methodology. Besides the evolution of the design of whole measuring systems, and including especially new applications of various detections methods, several aspects of implications of progress in nanotechnology, and miniaturization of measuring systems for application in different field of modern chemical analysis are also discussed.

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Section: Electrochemical Detectionsmentioning

confidence: 99%

Recent advances in flow injection analysis

Trojanowicz

Kołacińska

2016

Analyst

170

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“…The proposed sandwich assay can be performed via magnetic separationbased flow-injection analysis to achieve high-throughput measurements for practical applications. 51,52 Average value of three determinations ± standard deviation. b SPC stands for the standard plate count method using the Listeria chromogenic medium.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The proposed sandwich assay can be performed via magnetic separationbased flow-injection analysis to achieve high-throughput measurements for practical applications. 51,52…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Even though some biological species (such as proteins and histiocytes) in the samples may affect the recognition of the peptides to LM, these interferences can be reduced by selecting peptides with high binding affinities. The proposed sandwich assay can be performed via magnetic separation-based flow-injection analysis to achieve high-throughput measurements for practical applications. , …”

Section: Resultsmentioning

confidence: 99%

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Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay

Ding

Qin

2018

Anal. Chem.

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Peptide-based sandwich assays are promising tools in molecular detection, but may be restricted by the availability of "pairs" of affinity peptides. Herein, a new potentiometric sandwich assay for bacteria based on peptide pairs derived from an antimicrobial peptide (AMP) ligand is demonstrated. As a model, the original AMP with a welldefined structure for Listeria monocytogenes (LM) can be split into two fragments to serve as the peptide pairs for the sandwich assay. The recognition and binding of the short peptide pairs to the target can be verified by circular dichroism, flow cytometry, fluorometry, and optical microscopy. The potentiometric magnetic bead-based sandwich assay is designed by using horseradish peroxidase as a label. The enzyme can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine with H 2 O 2 to induce a potential change on a polymeric membrane ionselective electrode. Under optimal conditions, the concentration of LM can be determined potentiometrically in a linear range of 1.0 × 10 2 to 1.0 × 10 6 CFU mL −1 with a detection limit of 10 CFU mL −1 (3σ). The proposed sensing strategy expands the applications of peptides in the field of bioassays.

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Disposable Voltammetric Immunosensors Integrated with Microfluidic Platforms for Biomedical, Agricultural and Food Analyses: A Review

Felix

Baccaro

Angnes

2018

Sensors

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Disposable immunosensors are analytical devices used for the quantification of a broad variety of analytes in different areas such as clinical, environmental, agricultural and food quality management. They detect the analytes by means of the strong interactions between antibodies and antigens, which provide concentration-dependent signals. For the herein highlighted voltammetric immunosensors, the analytical measurements are due to changes in the electrical signals on the surface of the transducers. The possibility of using disposable and miniaturized immunoassays is a very interesting alternative for voltammetric analyses, mainly, when associated with screen-printing technologies (screen-printed electrodes, SPEs), and microfluidic platforms. The aim of this paper is to discuss a carefully selected literature about different examples of SPEs-based immunosensors associated with microfluidic technologies for diseases, food, agricultural and environmental analysis. Technological aspects of the development of the voltammetric immunoassays such as the signal amplification, construction of paper-based microfluidic platforms and the utilization of microfluidic devices for point-of-care testing will be presented as well.

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Flow Injection Analysis System Using Magnetic Beads, Screen Printed Electrodes and Magnets

Cited by 3 publications

References 25 publications

Recent advances in flow injection analysis

Recent advances in flow injection analysis

Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay

Disposable Voltammetric Immunosensors Integrated with Microfluidic Platforms for Biomedical, Agricultural and Food Analyses: A Review

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