Flow‐injection chemiluminescence determination of diazepam by oxidation with N‐bromosuccinimide

Abstract

A rapid and sensitive flow-injection chemiluminescence (FI-CL) method is described for the determination of diazepam based on its reaction with N-bromosuccinimide (NBS) in alkaline medium in the presence of dichlorofluorescein (DCF) as an effective energy-transfer agent. Under optimum conditions, the proposed method allowed the measurement of diazepam over the range of 2.0 × 10(-6) to 2.0 × 10(-4) mol/L with a detection limit of 5.0 × 10(-7) mol/L. The relative standard deviation for 11 parallel measurements of 2.0 × 10(-5) mol/L diazepam was 2.1%. The method was applied satisfactorily for the determination of diazepam in pharmaceutical preparations, and the results agree well with those obtained by spectrophotometry. The use of the proposed system for the determination of diazepam in urine and plasma samples was also tested. The possible mechanism of the chemiluminescence reaction is discussed briefly.

Benzodiazepines in complex biological matrices: Recent updates on pretreatment and detection methods

“…Platforms such as portable mobile devices [ 203 ] and others [ 204 ] improve the affinity of SERS for BDZs in complex matrices and the reliability of rapid on-site detection. CL method [ 200 ], with its improved technique [ 205 ], is also applied for the sensitive detection of BDZs by simple equipment at a fast signal detection speed. In terms of electrochemical methods, voltammetry provides high sensitivity without expensive apparatus compared with MS [ 79 ], and more electrodes with good electrochemical performance and stability are being developed as alternative options to achieve superior performance [ 196 ].…”