Flow-injection chemiluminescence determination of meloxicam by oxidation with N-bromosuccinimide

Liu, Haiyan; Zhang, Ling; Hao, Yuhong; Wang, Qingjiang; He, Pingang; Fang, Yuzhi

doi:10.1016/j.aca.2005.02.078

Cited by 22 publications

(17 citation statements)

References 31 publications

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“…The majority of these methods employ HPLC-ultraviolet (HPLC-UV) [2][3][4][5][6] or HPLCfluorescence (HPLC-FL) 10) spectrophotometry. Other reports employed LC-MS, 2) liquid chromatography-tandem mass spectrometry (LC-MS/MS), [7][8][9] flow injection analysis-chemiluminescence (FIA-CL), 11) or electrochemical methods. 12) Simple and rapid sample preparation is an advantage of HPLC.…”

Section: )mentioning

confidence: 99%

The Measurement of Meloxicam and Meloxicam Metabolites in Rat Plasma Using a High-Performance Liquid Chromatography-Ultraviolet Spectrophotometry Method

Miyamoto

Aoyama

Matsumoto

2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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A high-performance liquid chromatography-ultraviolet spectrophotometry (HPLC-UV) method for the determination of meloxicam (MEL) and meloxicam metabolites (5′-hydroxy meloxicam (5-HMEL) and 5′-carboxy meloxicam (5-CMEL)) has been developed. After extraction of MEL, 5-HMEL, and 5-CMEL from rat plasma using Oasis HLB cartridges, the extracts were separated with a Luna C18 (2) 100 A column (5 µm, 4.6 150 mm, Phenomenex) using a mobile phase of 50 mM phosphate buffer (pH 2.15, solvent A) and acetonitrile (solvent B) at a flow rate of 0.8 mL/min in a linear gradient. The detection wavelength was 360 nm, and the internal standard (IS) was piroxicam. Each calibration curve was linear in the range of 40 to 1000 ng/mL (r 2 >0.999). The extraction rates of MEL, 5-HMEL, and 5-CMEL were greater than 86.9%. The intra-and inter-day accuracies were in the range of 95.0 to 119.0%, and the precision was 0.2 to 17.0%. To the best of our knowledge, this is the first report of the quantitative and qualitative measurement of meloxicam and each metabolite using an HPLC-UV method. Key words meloxicam; meloxicam metabolite; HPLC-UVThe aim of this study was to establish a procedure to study the pharmacokinetic (PK) model of meloxicam (MEL). MEL (4-hydroxy-2-methyl-N-(5-methyl-2-thiazolyl)-2H-1,2-benzothiazine-3-carboxamide-1,1-dioxide) is a nonsteroidal anti-inflammatory drug (NSAID) that has selective inhibitory effects on the inducible isoform of cyclooxygenase-2. MEL is as effective as other NSAIDs in the treatment of rheumatoid arthritis and osteoarthritis in humans 1); it is used as an anti-inflammatory agent and analgesic in animals. 2)Generally, administrated meloxicam is first metabolized to a 5′-hydroxymethyl metabolite (5-HMEL) by CYP2C9 (major) and CYP3A4 (minor); it is then metabolized to a 5′-carboxy metabolite (5-CMEL). 3)Studies of the human plasma concentration/time profile of MEL after a single oral administration indicate that the graph exhibits bimodal peaks. 4,5) These reports suggest that MEL undergoes enterohepatic circulation. Taking these findings into account, we are planning to investigate the process by which MEL is metabolized to 5-HMEL and 5-CMEL (e.g., glucuronide conjugate, CYP metabolism). To investigate the PK model of MEL, measurement of the concentrations of MEL and its metabolites (5-HMEL and 5-CMEL) in biological samples is necessary. Several reports have examined the concentration of MEL in human and animal plasma [4][5][6][7][8][9] and the metabolism of MEL.2,3) However, these studies did not concomitantly measure 5-HMEL and 5-CMEL. The development of a system that enables the detection of MEL, 5-HMEL, and 5-CMEL is required.Various analytical methods for determining MEL in biological samples have been reported. The majority of these methods employ HPLC-ultraviolet (HPLC-UV) 2-6) or HPLCfluorescence (HPLC-FL) 10) spectrophotometry. Other reports employed LC-MS, 2) liquid chromatography-tandem mass spectrometry (LC-MS/MS), [7][8][9] flow injection analysis-chemiluminescence (FIA-CL), 11) or electroc...

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Section: )mentioning

confidence: 99%

The Measurement of Meloxicam and Meloxicam Metabolites in Rat Plasma Using a High-Performance Liquid Chromatography-Ultraviolet Spectrophotometry Method

Miyamoto

Aoyama

Matsumoto

2017

CHEMICAL & PHARMACEUTICAL BULLETIN

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“…Previous studies have shown that fluorescein accepted the energy generated from a redox reaction of NBS with analyte molecules, and was excited to produce CL (32)(33)(34)(35)(36). Previous studies have shown that fluorescein accepted the energy generated from a redox reaction of NBS with analyte molecules, and was excited to produce CL (32)(33)(34)(35)(36).…”

Section: Possible Mechanism Of CL Reactionmentioning

confidence: 99%

Flow‐injection chemiluminescence determination of diazepam by oxidation with N‐bromosuccinimide

2012

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A rapid and sensitive flow-injection chemiluminescence (FI-CL) method is described for the determination of diazepam based on its reaction with N-bromosuccinimide (NBS) in alkaline medium in the presence of dichlorofluorescein (DCF) as an effective energy-transfer agent. Under optimum conditions, the proposed method allowed the measurement of diazepam over the range of 2.0 × 10(-6) to 2.0 × 10(-4) mol/L with a detection limit of 5.0 × 10(-7) mol/L. The relative standard deviation for 11 parallel measurements of 2.0 × 10(-5) mol/L diazepam was 2.1%. The method was applied satisfactorily for the determination of diazepam in pharmaceutical preparations, and the results agree well with those obtained by spectrophotometry. The use of the proposed system for the determination of diazepam in urine and plasma samples was also tested. The possible mechanism of the chemiluminescence reaction is discussed briefly.

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“…N-bromosuccinimide (NBS) also has been successfully introduced into CL reactions and developed for drugs analysis using NBS as a CL oxidant. [21][22][23][24][25] In aqueous solutions, its oxidizing properties were attributed to hypobromous acid generated by its hydrolysis. NBS has similar oxidation properties to hypobromite, but is more stable.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence Determination of Gossypol by Oxidation with N‐Bromosuccinimide

Han

Fang

Wei

2013

J Chinese Chemical Soc

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A chemiluminescence (CL) phenomenon was observed when gossypol was injected into a reaction mixture of N-bromosuccinimide (NBS) and alkaline dichlorofluorescein (DCF). Based on this phenomenon, a rapid and sensitive method for the determination of gossypol was established. Under the optimum conditions, the linear range was from1.0 × 10 -9 to 1.0 × 10 -6 M. The detection limit was 1.0 × 10 -10 M. The method has been applied to the determination of gossypol in cottonseed, cottonseed oil, pharmaceutical and biological fluids with satisfactory results. The possible CL reaction mechanism was discussed briefly.

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Flow-injection chemiluminescence determination of meloxicam by oxidation with N-bromosuccinimide

Cited by 22 publications

References 31 publications

The Measurement of Meloxicam and Meloxicam Metabolites in Rat Plasma Using a High-Performance Liquid Chromatography-Ultraviolet Spectrophotometry Method

The Measurement of Meloxicam and Meloxicam Metabolites in Rat Plasma Using a High-Performance Liquid Chromatography-Ultraviolet Spectrophotometry Method

Flow‐injection chemiluminescence determination of diazepam by oxidation with N‐bromosuccinimide

Chemiluminescence Determination of Gossypol by Oxidation with N‐Bromosuccinimide

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