Flow injection determination of lactate based on a photochemical reaction using photometric and chemiluminescence detection

Pérez-Ruíz, Tomás; Martı́nez-Lozano, Carmen; Martı́n, Jesús

doi:10.1039/a904497c

Cited by 18 publications

(5 citation statements)

References 15 publications

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“…Currently, in the scientific literature, there are no studies published on lactic acid in fingerprints. However, one article by Pérez‐Ruiz discussed the decomposition of lactate with an unidentified degradation product in presence of iron upon irradiation with UV or visible light (14). The pyruvic acid M ·+ ion at m/z 87 was monitored in all the samples analyzed and correlated with the amount of lactic acid.…”

Section: Resultsmentioning

confidence: 99%

Photo‐ and Thermal‐Degradation Studies of Select Eccrine Fingerprint Constituents

Paoli

Lewis

et al. 2010

Journal of Forensic Sciences

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Photo- and thermal-degradation studies on eccrine fingerprint components are presented herein. Dilute distinct solutions of urea, lactic acid, and seven amino acids were deposited on steel coupons and Teflon disks, exposed to artificial sunlight or heat, extracted, and analyzed. This aim of this study was to determine whether the investigated eccrine components, previously determined to be Raman active for a parallel study, experienced photo- or thermally induced degradation, and if so, to determine the rate and identify any detectable products. Neither the amino acids nor urea exhibited photo-degradation; however, when heated for a period of three minutes, the onset of thermal-degradation was initiated at 100 degrees C for the amino acids and 100 degrees C for urea. Lactic acid, the major polymerization initiator of superglue fuming, showed photochemical and thermal-degradation. These results could be used for future development of new latent fingerprint visualization methods, especially when lactic acid is degraded.

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Section: Resultsmentioning

confidence: 99%

Photo‐ and Thermal‐Degradation Studies of Select Eccrine Fingerprint Constituents

Paoli

Lewis

et al. 2010

Journal of Forensic Sciences

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“…During ordinary metabolic activity with sufficient oxygen supply, the reduction of pyruvate to lactate is poorly active in normal cells, which usually degrade pyruvate to CO 2 through the TCA cycle and re-oxidize NADH through the mitochondrial respiratory chain. This can be also deduced by the low blood lactate concentration measured in normal, resting individuals (<1 mM) [22]. In normal cells, a rapid NAD+ regeneration, as that afforded by the LDH reaction, could usefully contribute to avoid perturbations of the redox status.…”

Section: Biological Functions Of Ldh In Normal Cellsmentioning

confidence: 99%

Inhibition of Lactate Dehydrogenase Activity as an Approach to Cancer Therapy

et al. 2014

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In the attempt of developing innovative anticancer treatments, growing interest has recently focused on the peculiar metabolic properties of cancer cells. In this context, LDH, which converts pyruvate to lactate at the end of glycolysis, is emerging as one of the most interesting molecular targets for the development of new inhibitors. In fact, because LDH activity is not needed for pyruvate metabolism through the TCA cycle, inhibitors of this enzyme should spare glucose metabolism of normal non-proliferating cells, which usually completely degrade the glucose molecule to CO2. This review is aimed at summarizing the available data on LDH biology in normal and neoplastic cells, which support the anticancer therapeutic approach based on LDH inhibition. These data encouraged pharmaceutical industries and academic institutions in the search of small-molecule inhibitors and promising candidates have recently been identified. The availability of inhibitors with drug-like properties will allow the evaluation in the near future of the real potential of LDH inhibition in anticancer treatment, also making the identification of the most responsive neoplastic conditions possible.

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“…Lactate (2‐hydroxypropanoate) is very important in food analysis for assessing the freshness and stability of the products. In addition, lactic acid is applied topically for the treatment of hyperkeratotic skin disorders and verrucae 181. Gomes et al182 have described the construction and evaluation of a sol–gel biosensor coupled to a sequential injection system to accomplish the quantification of lactate in pharmaceutical products.…”

Section: Representative Studies Involving Amperometric Detection and mentioning

confidence: 99%

Fast and Accurate Analysis of Drugs Using Amperometry Associated With Flow Injection Analysis

Felix

Angnes

2010

Journal of Pharmaceutical Sciences

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Flow injection determination of lactate based on a photochemical reaction using photometric and chemiluminescence detection

Cited by 18 publications

References 15 publications

Photo‐ and Thermal‐Degradation Studies of Select Eccrine Fingerprint Constituents

Photo‐ and Thermal‐Degradation Studies of Select Eccrine Fingerprint Constituents

Inhibition of Lactate Dehydrogenase Activity as an Approach to Cancer Therapy

Fast and Accurate Analysis of Drugs Using Amperometry Associated With Flow Injection Analysis

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