Flow injection Fluorimetric Determination of Ascorbic Acid Based on its Photooxidation by Thionine Blue

Martı́nez-Lozano, Carmen; Pérez-Ruíz, Tomás; Sidrach, Ciriaco

doi:10.1039/a606841c

Cited by 24 publications

(11 citation statements)

References 34 publications

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“…Until now, Lascorbic acid has been determined by many analytical methods. For example, determinations using atomic absorption, 4 flow injection, [5][6][7][8][9][10][11][12] electrochemiluminescence, 13 electrochemistry 14 and spectrophotometric analyses, [15][16][17][18] have been reported. [19][20][21][22][23][24][25] Moreover, as an L-ascorbic acid analysis using CL, the determination by flow injection with permanganate was reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of Small Amounts of L-Ascorbic Acid Using the Chemiluminescence of an Iron-Chlorophyllin Complex

et al. 2005

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Section: Introductionmentioning

confidence: 99%

Determination of Small Amounts of L-Ascorbic Acid Using the Chemiluminescence of an Iron-Chlorophyllin Complex

et al. 2005

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“…2 FIA is the most frequently used method for AA determination. Most of the proposed FIA methods are based on UV-visible spectrophotometry, 3 amperometry, 4 chemiluminscence, 5 spectrofluorometry 6 and voltammetry, 7 to mention recent references in each case.…”

Section: Introductionmentioning

confidence: 99%

Integrated FIA for the Determination of Ascorbic Acid and Dehydroascorbic Acid in a Microfabricated Glass-Channel by Thermal-Lens Microscopy

et al. 2000

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An on-chip chemical micro-determination device, integrating the FIA system, was applied to L-ascorbic acid (AA) and dehydroascorbic acid (DHAA) determination. Microchannels having a 250×100 µm cross-section were fabricated in a quarts glass chip as a component in an integrated FIA system. They were put to use for flow, mixing, reaction, and detection. The reaction system was a coupled redox-complexation reaction between AA and a 1,10-phenantroline-Fe(III) mixture. Sample solutions were introduced into micro-channels by micro-syringes, the flow rate was controlled by a micro-syringe pump, and the reagents, were mixed by molecular diffusion. A photothermal microscope was used for the ultra-sensitive detection of the non-fluorescent reaction product (ferroin). For DHAA determination, dithiothreithol was used as a reducing agent and the total AA was determined. The DHAA quantity was calculated by subtraction. This method allows for the determination of AA with a linear range of up to 40 µM. The RSD for 25 µM AA (n=5) is 1%, and the limit of detection (LOD) is 0.1 µM. The procedure was successfully applied to determining the AA and DHAA content of urine and AA in vitamin C tablets. The results agree well with those obtained by reference methods.

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“…In a similar study, 8) thionine blue was reacted with AA under the light. The product of reaction between AA and thionine blue called Leucothionine blue was highly fluorescent characterized, which could be easily used for the sensitive determination of AA in a flow injection system.…”

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confidence: 99%

“…In another study, 8) Thionine blue is reacted with AA in order to develop a simple, sensitive, selective and rapid fluorimetric method for determining AA. The product of this photochemical reaction, Leucothionine blue, is a highly florescent specimen, which is used for sensitive determination of AA in flow injection system.…”

mentioning

confidence: 99%

“…Many methods depend on the reaction of AA by means of suitable reagents, then the product of reaction or excess reagent monitored with optically (spectrophotometric, [2][3][4] fluorimetric [5][6][7][8] or chemiluminimetric 9) ), electrochemically (amperometric, 10,11) voltammetric, 12,13) potentiometric 14) or conductometric 15) ) and chromatographically (High Pressure Liquid Chromatography 16,17) ). Spectrofluorimetric analysis is well known for its sensitivity and speed.…”

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Fluorimetric Determination of Ascorbic Acid in Vitamin C Tablets Using Methylene Blue

Dilgin

Nişli

2005

Chem. Pharm. Bull.

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Ascorbic acid (AA), also known as Vitamin C, is a watersoluble vitamin and needed for the growth and repair of tissues in all parts of the body. AA has vital importance in processes of oxidation and reduction in human organism participating of several metabolic reactions. AA has also been used for the prevention and treatment of the common cold, mental illness, infertility, cancer and AIDS.1) Due to the great importance of AA in human beings, the quantitative analysis of AA has gained increased significance in several areas of analytical chemistry such as pharmaceutical and food applications. It is also clinically important to determine its concentration in blood, urine and some tissues.Many methods depend on the reaction of AA by means of suitable reagents, then the product of reaction or excess reagent monitored with optically (spectrophotometric, [2][3][4] fluorimetric [5][6][7][8] or chemiluminimetric 9) ), electrochemically (amperometric, 10,11) voltammetric, 12,13) potentiometric 14) or conductometric 15) ) and chromatographically (High Pressure Liquid Chromatography 16,17) ). Spectrofluorimetric analysis is well known for its sensitivity and speed. Recently, fluorimetric determinations of AA have been developed based on the condensation reactions of AA with o-phenylenediamine 5) and on the oxidation with mercury(II) of vitamin C to form quinoxaline derivate.6) The reaction products of these methods exhibit fluorescence. A simple, sensitive and selected fluorimetry for determination of AA is based on the reaction of AA in alkaline medium with 2,3-diaminonaphthalene without oxidant.7) The addition of beta-cyclodextrin (beta-CD) was greatly enhancing the fluorescence of the system. In another study, 8) Thionine blue is reacted with AA in order to develop a simple, sensitive, selective and rapid fluorimetric method for determining AA. The product of this photochemical reaction, Leucothionine blue, is a highly florescent specimen, which is used for sensitive determination of AA in flow injection system.In our study, Methylene Blue (MB), a member of thiazine dye groups like thionine blue, was used for the fluorimetric determination of AA. MB is widely used in a number of different areas e.g. a photo sensitizer to produce singlet oxygen in photodynamic therapy for the treatment of cancer. The highly colored oxidized form of MB can be reduced to the colorless leuco form Leuco-Methylene Blue (LMB), as shown in Fig. 1.18) The redox indicator properties of this couple have been used extensively in chemical analyses. The reaction between AA and MB was used for AA determination in pharmaceuticals or fruit juices by using spectrophotometry, 19) amperometry 20,21) or voltammetry. 22)In this study, the determination of AA is based on the measurement of decreasing of the fluorescence intensity of MB due to the reaction between AA and MB, where MB is reduced to colorless LMB and AA is oxidized to dehydroascorbic acid (DHAA). This method allows us to determine the amount of AA in the purified materials, specifically Vit...

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Flow injection Fluorimetric Determination of Ascorbic Acid Based on its Photooxidation by Thionine Blue

Cited by 24 publications

References 34 publications

Determination of Small Amounts of L-Ascorbic Acid Using the Chemiluminescence of an Iron-Chlorophyllin Complex

Determination of Small Amounts of L-Ascorbic Acid Using the Chemiluminescence of an Iron-Chlorophyllin Complex

Integrated FIA for the Determination of Ascorbic Acid and Dehydroascorbic Acid in a Microfabricated Glass-Channel by Thermal-Lens Microscopy

Fluorimetric Determination of Ascorbic Acid in Vitamin C Tablets Using Methylene Blue

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