Flow-injection immunoanalysis, a versatile and powerful tool for the automatic determination of environmental pollutants

Zhi, Zhang

doi:10.1002/(sici)1098-2728(1999)11:2<83::aid-lra3>3.0.co;2-h

Cited by 3 publications

References 27 publications

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Chromatography, Affinity

Hage¹

2003

Kirk-Othmer Encyclopedia of Chemical Technology

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Affinity chromatography is a liquid chromatographic technique that uses a biologically related ligand for the specific retention or analysis of a given target. The general theory of affinity chromatography is considered and several examples are given of its typical applications. A number of items to consider in the use of this method are discussed, including the choice of ligand, support material, immobilization method, and solvent conditions. Several types of affinity chromatography are then examined in more detail. Particular attention is given to the techniques of bioaffinity chromatography, immunoaffinity chromatography, boronate affinity chromatography, immobilized metal‐ion affinity chromatography, dye‐ligand affinity chromatography, and biomimetic affinity chromatography. The use of affinity chromatography in the measurement of thermodynamic and kinetic constants is also considered, as well as the use of this method in sample pretreatment, purification, and analysis. Other items that are discussed include recent developments in the use of molecular imprints as affinity ligands and the use of biosensors for the study of biological interactions.

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Chromatography, Affinity

Hage¹

2003

Kirk-Othmer Encyclopedia of Chemical Technology

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Environmental Water Samples Analysis of Pesticides by Means of Chemometrics Combined with Fluorimetric Multioptosensing

2007

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A single flow-through optosensor spectrofluorimetric system is proposed for the resolution of mixtures of three pesticides, alpha-naphthol, o-phenylphenol and thiabendazole, at microg l(-1) levels using a partial least-squares (PLS) calibration approach. The sensor was developed in conjunction with a monochannel flow-injection analysis system with fluorimetric detection using C18 silicagel as an active sorbent substrate in the flow cell. By using 20% methanol-water (v:v) solution as carrier solution, the multisensor responds linearly in the measuring range without requiring additional reagents or derivatization. First derivative emission spectra of the corresponding analytes recorded during the process of retention-elution were used to provide multivariate data. The different kinetic on the retention process of the analytes on the sensing zone allows the selection of a time matrix for each analyte providing best results in the PLS approach. Accurate prediction results were obtained for the three analytes with RMSEP values of 1.86%, 3.34% and 0.50% were obtained for alpha-naphthol, o-phenylphenol and thiabendazole respectively. In the analysis of environmental waters samples, a mean recovery of 103% was obtained.

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Desenvolvimento de sistemas de análises em fluxo por multicomutação para determinação de poluentes ambientais

Córdova¹

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Flow-injection immunoanalysis, a versatile and powerful tool for the automatic determination of environmental pollutants

Abstract: Flow-injection immunoanalysis (FIIA) is a novel and automatic

Cited by 3 publications

References 27 publications

Chromatography, Affinity

Chromatography, Affinity

Environmental Water Samples Analysis of Pesticides by Means of Chemometrics Combined with Fluorimetric Multioptosensing

Desenvolvimento de sistemas de análises em fluxo por multicomutação para determinação de poluentes ambientais

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