Flow-injection spectrofluorometric determination of trace amounts of formaldehyde in water after derivatization with acetoacetanilide

Abstract

A novel fluorophotometric method for formaldehyde determination in environmental waters was developed: the method does not require any enrichment procedures. A flow injection analysis method for the spectrofluorometric determination of formaldehyde in waters, which is based on the reaction of formaldehyde with acetoacetanilide and ammonia, is proposed. The proposed method shows a good linearity from 0.50 to 40 x 10 -7 M, and the limit of detection (LOD) of 3 x 10 -9 M (0.09 ppb) is achievable. The sample throughput is 15 h -1 . One of the main advantages in the proposed method is that the reaction can be carried out at room temperature without any heating system. The effect of 1 various interferences possibly present in the real water samples was investigated. Most cations and anions, as well as organic compounds, do not interfere with the determination of formaldehyde in environmental water samples. The proposed method is very simple, rapid, less expensive, and highly sensitive, and can be applied to the environmental water samples, such as rain, tap water and river water, at low concentration levels without any enrichment procedure.

Development of novel detection reagent for simple and sensitive determination of trace amounts of formaldehyde and its application to flow injection spectrophotometric analysis

“…In order to evaluate the accuracy of the proposed method, the results obtained were compared with those obtained with an acetylacetone/spetrophotometric method and acetoacetanilide/fluorometric method described in the previous papers [30,38]. Rainwater samples 1, 2, and 3 were collected in Okayama University campus in the different day in December 2006.…”

“…Analytical results for the determination of formaldehyde in natural water samples a Acetylacetone/spetrophotometric method and acetoacetanilide/fluorometric method were described in the previous papers [30,38]. All values are means (n = 5) with ± σ (standard deviation).…”

“…Such fluorometric methods for formaldehyde determination require high reaction temperatures, so that high backpressure, a postcooling device or a debubbling diffusion cell are necessary to prevent the bubble generation and the increase in consequent noise. Recently, a flow injection fluorometric detection method with acetoacetanilde was developed by the authors [38]. The method can be carried out at room temperature; the detection limit is 3 x 10 -9 M (0.09 μg L -1 ).…”

Determination of formaldehyde by flow injection analysis with spectrophotometric detection exploiting brilliant green–sulphite reaction

“…Accordingly, other methods have been studied, seeking not only a rapid determination but also lower limits of quantification (Fagnani et al, 2003;Gigante et al, 2004;Abbasi et al, 2007;Afkhami & Bagheri, 2012). Techniques such as spectrophotometry (Zhang et al, 2004) and fluorescence (Li et al, 2007) can be readily automated by using flow injection analysis (FIA), thereby minimising analyst intervention, increasing the sampling rate, improving the precision of the determinations and using lower volumes of sample and reagents (Zhang et al, 2004). As a consequence, different spectrophotometric-FIA methods have been investigated for FA determination.…”

Membrane Solubilization Technique for Spectrophotometric Determination of Trace Formaldehyde in Rainwater

“…Though these methods are sensitive and reliable, their pretreating procedures are timeconsuming and cumbersome. Recently, simple and sensitive flow-injection fluorescence methods have been proposed, [14][15][16] but they required purpose-built instruments.…”