Flow Probes for NMR Spectroscopy

Haner, Ronald L.; Keifer, Paul A.

doi:10.1002/9780470034590.emrstm1085

Cited by 6 publications

(8 citation statements)

References 31 publications

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“…In the current study using the H1NMR spectra phosphate residues were detected at the 0.1 ppm. Furthermore, correlation signal was observed for a C2H-R3 (H1) peak at 4-5.1 ppm [22,26]. According to the Vinogradov and his colleague's study, a glycerol teichoic acid H1NMR spectrum has shown N-acetyl glucosamine residues at the 2.1-2.3 ppm (H2-H6) as well as glycerol residues at 3.8-4 ppm which our data confirmed the obtained results in previous researches [26].…”

Section: Resultssupporting

confidence: 90%

“…The spectra (NMR) of Gly-TA show signals attributable to (CH3)-3HPO4 residues at the 0.4 ppm. Similar data has been obtained in previous studies [22,25] (Details have been shown in Fig. 3).…”

Section: Resultssupporting

confidence: 90%

“…Total carbohydrate content was determined by phenolsulphuric acid [20], using a standard curve of glucose. Finally, recovered Gly-TA was analyzed by Fourier Transform Infrared spectroscopy (FTIR) [21] (TENSOR 27 Burker instrumental averaging of 256 scans on the FTIR spectrometer) and Proton Nuclear Magnetic Resonance spectroscopy (H1NMR) according to established methods [22]. The structural formula of isolated Gly-TA from lactobacillus was used as a reference for analyzing FTIR and H1NMR patterns (http://micro.digitalproteus.com/morphology3.php).…”

Section: Confirmation Representative Gly-ta By Ftir and H1nmrmentioning

confidence: 99%

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Purification and evaluation of glycerol teichoic acid from biofilm forming Staphylococcus epidermidis as a putative vaccine candidate

Mirzaei

Moosavi

Babaei

et al. 2015

vacres

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Introduction: Biofilm forming Staphylococcus epidermidis is a main causative agent of infections related to medical devices. Purification and evaluation of Gly-TA polysaccharide from a biofilm-forming S. epidermidis as a putative vaccine candidate were the main goals of the current study. Methods: Taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (Gly-TA) was purified from the above-mentioned strain and biochemical analyses including, Fourier Transform Infrared spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance spectroscopy (H1-NMR) were conducted for the recovered polysaccharide. Results: Following PCR confirmation of a S. epidermidis strain, Gly-TA was extracted and its biochemical compositions (i.e. N-acetyl glucose amine residues) were obtained. Conclusion: It is envisaged that Gly-TA polysaccharide could be considered as a putative vaccine to inhibit formation of biofilm by S. epidermidis.

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Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 90%

Section: Confirmation Representative Gly-ta By Ftir and H1nmrmentioning

confidence: 99%

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Purification and evaluation of glycerol teichoic acid from biofilm forming Staphylococcus epidermidis as a putative vaccine candidate

Mirzaei

Moosavi

Babaei

et al. 2015

vacres

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“…Two classes of flow probes have been designed depending on the position of the sample tube, which can be vertical (denoted below as type 1) or horizontal (denoted below as type 2) ( Fig. 1 ), and the shape of the RF coil for transmitting and receiving, namely saddle-shaped when the sample tube is placed vertically and solenoidal when the sample tube is placed horizontally [ 14 ]. Manufacturers have designed commercial NMR flow probes of type 1 that can be integrated into their high and low-resolution NMR instruments.…”

Section: Reviewmentioning

confidence: 99%

“…For the design of a NMR flow probe, similarly to the design of a new probe head, careful choice of the components should be made in order to have an optimal resolution, sensitivity and RF homogeneity, but in addition, other factors should be taken into account because of working on-flow. Hence, the design should allow a high filling factor, the flash out of air bubbles and the displacement of the existing fluid in the detection volume by the incoming fluid instead of just mix with it, among other issues [ 14 ]. Hence, the flow cell is a sample tube (made of glass or quartz) with openings at the top (outlet) and at the bottom (inlet) to enable connection to the flow system.…”

Section: Reviewmentioning

confidence: 99%

NMR reaction monitoring in flow synthesis

Gómez¹,

Hoz²

2017

Beilstein J. Org. Chem.

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Recent advances in the use of flow chemistry with in-line and on-line analysis by NMR are presented. The use of macro- and microreactors, coupled with standard and custom made NMR probes involving microcoils, incorporated into high resolution and benchtop NMR instruments is reviewed. Some recent selected applications have been collected, including synthetic applications, the determination of the kinetic and thermodynamic parameters and reaction optimization, even in single experiments and on the μL scale. Finally, software that allows automatic reaction monitoring and optimization is discussed.

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