A numerical investigation was carried out to explore the effects of a dielectric barrier discharge (DBD) plasma actuator on a three-dimensional incompressible, separated flow. The test article selected for the simulations was the National Aeronautical and Space Administration (NASA) wall-mounted hump model. The simulations were run at a Reynolds number of 936,000, based on hump chord length, and a freestream Mach number of 0.1. Hybrid partially averaged Navier–Stokes/large-eddy simulations (PANS/LES) were completed using CALC-LES, a well-validated computational fluid dynamics (CFD) code, developed by Chalmers University of Technology. The baseline code was modified to simulate the effects of the actuator, which were modeled as source terms in the momentum equation and were assumed to be steady and constant in the span-wise direction. The numerical simulations were carried out for a baseline (no control) case and five plasma control cases. To optimize the performance of the actuator, the variation of actuator location and voltage frequency was investigated. For the baseline case, a comparison of time-averaged skin friction, the coefficient of pressure, and velocity profiles was made of the available experimental results. The results of the baseline case showed good agreement for a hybrid turbulence model, thus strengthening the solver’s ability to predict a three-dimensional separated flow with reasonable accuracy. The results with the plasma actuator turned on showed improved flow characteristics compared to the baseline simulations by reducing the region of separated flow. The actuator placed just downstream of the separation point at an operational frequency of 5kHz completely eliminated the separated flow for our test conditions.