Flow-type microbial chip for screening of a single bacterium

Kano, Tomonori; Inaba, Tomomi; Higashi, Kazuhiko; Miki, Norihisa

doi:10.1049/mnl.2012.0556

Cited by 3 publications

(1 citation statement)

References 20 publications

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“…However, there are strong needs for the microstructures with this size. For example, in our prior work, we attempted to immobilize bacteria in micro holes using dielectrophoresis for precise characterization of them 11) since bacteria have wide varieties of industrial applications, such as production of food, drug, and other useful chemicals. Since their sizes are on the order of 1 m, when we used micro holes that can be manufactured by UV lithography, i.e., greater than 3 m, more than one bacteria were immobilized in the hole and the number of immobilized bacteria had large variation among the holes.…”

Section: Introductionmentioning

confidence: 99%

Microfabrication of Super Absorbent Polymer Structure Using Nanoimprinting and Swelling Process

Inaba

Kano

Miki

2013

Jpn. J. Appl. Phys.

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We consider a physical scenario for ultra-relativistic heavy-ion collisions where, at the early stage, only transverse degrees of freedom of partons are thermalized, while the longitudinal motion is described by free streaming. When the energy density of the partonic system drops to a certain critical value, the partons hadronize and the newly formed hadronic system freezes out. This sudden change is described with the help of the Landau matching conditions followed by the simulations done with THERMINATOR. The proposed scenario reproduces well the transverse-momentum spectra, the elliptic flow coefficient v 2 and the HBT radii of pions and kaons studied at RHIC (Au + Au collisions at √ s NN = 200 GeV). It also reproduces quite well 0954-3899/10/094023+06$30.00

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Section: Introductionmentioning

confidence: 99%

Microfabrication of Super Absorbent Polymer Structure Using Nanoimprinting and Swelling Process

Inaba

Kano

Miki

2013

Jpn. J. Appl. Phys.

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Development of hydrogel microtubes for microbe culture in open environment

Ogawa

Higashi

Miki

2015

2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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This paper describes a microbe culture system in an open environment using hydrogel microtubes. In recent years, oil production microbes, such as Aurantiochytrium, have been found and are studied to produce fuels of new age instead of fossil fuels. Biomass production by microbes is promising, where scale-up, collection of the products and competition against other microbes are the most important challenges. Here, we propose to use hydrogel microtubes to encapsulate, culture, and protect microbes. The tubes can be micro- and mass-fabricated. They allow oxygen and nutrition to go through while they prevent competitive microbes from intruding inside. The microbes and byproducts can be collected together with the tubes. In this paper, we demonstrate the proof-of-concepts experiments: we fabricated hydrogel micro tubes and cultured Coryne glutamicum which produce lactic acid inside the tubes. The microbes were increased inside the tubes and protected even when competitive microbes existed in the culture media. Furthermore, we demonstrated how to collect microbes inside the tubes.

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Micro-tube mass production device for microbial culture

Fujimoto

Ogawa

Higashi

et al. 2016

2016 38th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

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This paper describes mass production system of micro-tubes for microbial culture in an open environment. Microbes are used in many fields, such as food, medicine, environmental and energy. We proposed a microbe culture system using hydrogel micro-tubes, which can protect the target microbes inside from competitive microbes outside of the tubes while allow oxygen and nutrition to diffuse through. The hydrogel micro-tubes can be produced by a microfluidic device, which can precisely control the flow and therefore, the tube geometry. For practical applications of the micro-tube-based microbial culture, one of the biggest challenges is the scale-up of the micro-tube-based culture system, or mass production of the tubes. We developed a fluidic system that can produce multiple micro-tubes in parallel. We characterized the mass-produced micro channels and verified the effectiveness of the system.

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Flow-type microbial chip for screening of a single bacterium

Cited by 3 publications

References 20 publications

Microfabrication of Super Absorbent Polymer Structure Using Nanoimprinting and Swelling Process

Microfabrication of Super Absorbent Polymer Structure Using Nanoimprinting and Swelling Process

Development of hydrogel microtubes for microbe culture in open environment

Micro-tube mass production device for microbial culture

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