Flower Colour Modification of Chrysanthemum by Suppression of F3'H and Overexpression of the Exogenous Senecio cruentus F3'5'H Gene

He, Hua; Hu, Ke; KeTing, Han; Qiaoyan, Xiang; Dai, Silan

doi:10.1371/journal.pone.0074395

Cited by 79 publications

(46 citation statements)

References 51 publications

(61 reference statements)

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“…By expressing genes in the Petunia that were deficient in the F3 ′ H and F3 ′ 5 ′ H genes, Tanaka reported that the CYP75 members from cineraria are F3′H and F3′5′H (Tanaka and Brugliera, 2013). However, in accordance with our previous studies, the results from our gene expression and HPLC analysis show that the content of Dp was much higher than Cy in JeB, suggesting that there is a very high CYP75A activity of ScF3 ′ 5 ′ H (Huang et al, 2013; Sun et al, 2014). …”

Section: Discussionsupporting

confidence: 93%

Transcriptomics and Metabolite Analysis Reveals the Molecular Mechanism of Anthocyanin Biosynthesis Branch Pathway in Different Senecio cruentus Cultivars

Jin

Wang

et al. 2016

Front. Plant Sci.

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The cyanidin (Cy), pelargonidin (Pg), and delphinidin (Dp) pathways are the three major branching anthocyanin biosynthesis pathways that regulate flavonoid metabolic flux and are responsible for red, orange, and blue flower colors, respectively. Different species have evolved to develop multiple regulation mechanisms that form the branched pathways. In the current study, five Senecio cruentus cultivars with different colors were investigated. We found that the white and yellow cultivars do not accumulate anthocyanin and that the blue, pink, and carmine cultivars mainly accumulate Dp, Pg, and Cy in differing densities. Subsequent transcriptome analysis determined that there were 43 unigenes encoding anthocyanin biosynthesis genes in the blue cultivar. We also combined chemical and transcriptomic analyses to investigate the major metabolic pathways that are related to the observed differences in flower pigmentation in the series of S. cruentus. The results showed that mutations of the ScbHLH17 and ScCHI1/2 coding regions abolish anthocyanin formation in the white and the yellow cultivars; the competition of the ScF3′H1, ScF3′5′H, and ScDFR1/2 genes for naringenin determines the differences in branching metabolic flux of the Cy, Dp, and Pg pathways. Our findings provide new insights into the regulation of anthocyanin branching and also supplement gene resources (including ScF3′5 ′H, ScF3′H, and ScDFRs) for flower color modification of ornamentals.

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Section: Discussionsupporting

confidence: 93%

Transcriptomics and Metabolite Analysis Reveals the Molecular Mechanism of Anthocyanin Biosynthesis Branch Pathway in Different Senecio cruentus Cultivars

Jin

Wang

et al. 2016

Front. Plant Sci.

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“…The two genes are closely related, having diverged from a common ancestor, and reversals of function are known in nature (Seitz et al 2006). Senecio cruentus F3′5′H for instance, when transformed into Chrysanthemum, fails to produce delphinidin but increases cyanidin production (He et al 2013). What is more surprising is the complete lack of delphinidin production by wild-type Lathyrus F3′5′H when transformed into Arabidopsis background.…”

Section: Discussionmentioning

confidence: 99%

“…(iii) Arabidopsis is not an appropriate host for sweet pea F3′5′H for reasons other than the lack of a suitable reductase suggested above. Studies have shown that gene origin is important to the successful transformation of F3′5′H with some host-donor pairs more successful in producing delphinidin-series than others (Okinaka et al 2003;Katsumoto et al 2007;He et al 2013). One possibility is that substrate specificity for DFR is different between sweet pea and the Arabidopsis host.…”

Section: Discussionmentioning

confidence: 99%

The molecular basis for an ancient colour mutant in sweet pea (Lathyrus odoratus)

Xue

Cronk

2018

Can. J. Plant Sci.

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The classic A1 locus in sweet pea (Lathyrus odoratus) was investigated by Bateson, Punnett, and Saunders in the early 20th century history of Mendelian genetics. The mutation, in the form of the pink and white cultivar 'Painted Lady', is known from the 18th century. We show that this locus is associated with a single base pair mutation (332 G/A) in the flavonoid 3′,5′-hydroxylase (F3′5′H) gene. This results in an amino acid change (111 glycine/ aspartic acid) in the conserved substrate recognition site 1 of the enzyme. The mutant flower lacks the blue pigment delphinidin and is thus pink and white, rather than purple and blue as in the wild-type. This single amino acid change at a functionally important site appears to convert the enzyme from primary F3′5′H activity to a relatively efficient F3′H, as suggested by heterologous transformation into Arabidopsis PAP1D (a mutant line that produces anthocyanin constitutively).Résumé : Bateson, Punnet et Saunders ont étudié le locus A1 classique du pois de senteur (Lathyrus odoratus) au début du 20 e siècle, quand la génétique mendélienne en était à ses débuts. La mutation, en l'occurrence le cultivar rose et blanc Painted Lady, remonte au 18 e siècle. Les auteurs montrent que le locus est associé à la mutation d'un seule paire de bases (332 G/A) du gène codant la flavonoïde 3′,5′-hydroxylase (F3′5′H). Cette mutation entraîne la modification d'un acide aminé (111 glycine/acide aspartique) au site 1 de reconnaissance du substrat (SRS1) de l'enzyme. La fleur mutante est dépourvue de delphinidine, le pigment bleu, ce qui lui confère une couleur rose et blanche, plutôt que mauve et bleue, comme chez le type sauvage. La modification de cet unique acide aminé à un site fonctionnellement important semble transformer la protéine F3′5′H, à l'activité enzymatique rudimentaire, en protéine F3′H passablement efficace, comme le suggère la transformation hétérologue de la lignée PAP1D d'Arabidopsis (lignée mutante qui synthétise par définition de l'anthocyanine). [Traduit par la Rédaction]

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“…These structural genes play an important role in the synthesis and accumulation of anthocyanins and the homologues genes have been isolated in many ornamental plants including Petunia, Chrysanthemum, Gentiana, Gerbera, and Torenia (Koes et al, 1986;Van Tunen et al, 1988;Nakamura et al, 2006;Wellmann et al, 2006;Nakatsuka et al, 2008;He et al, 2013). Meanwhile, genetic engineering of flower color has been achieved through transgenic experiments.…”

Section: Lathyroides (Lath) Wox1mentioning

confidence: 99%

Evolution and developmental genetics of floral display—A review of progress

Zhang

Xiang

2017

J of Sytematics Evolution

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Angiosperms evolved a great diversity of ways to display their flowers for reproductive success by variation in floral color, size, shape, scent, arrangements, and flowering time. The various innovations in floral forms and the aggregation of flowers into different kinds of inflorescences can drive new ecological adaptations, speciation, and angiosperm diversification. Evolutionary developmental biology (evo-devo) seeks to uncover the developmental and genetic basis underlying morphological diversification. Advances in the developmental genetics of floral display have provided a foundation for insights into the genetic basis of floral and inflorescence evolution. A number of regulatory genes controlling floral and inflorescence development have been identified in model plants (e.g., Arabidopsis thaliana, Antirrhinum majus) using forward genetics and conserved functions of many of these genes across diverse non-model species have been revealed by reverse genetics. Gene-regulatory networks that mediated the developmental progresses of floral and inflorescence development have also been established in some plant species. Meanwhile, phylogeny-based comparative analysis of morphological and genetic character has enabled the identification of key evolutionary events that lead to morphological complexity and diversification. Here we review the recent progress on evo-devo studies of floral display including floral symmetry, petal fusion, floral color, floral scent, and inflorescences. We also review the molecular genetic approaches applied to plant evo-devo studies and highlight the future directions of evo-devo.

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Flower Colour Modification of Chrysanthemum by Suppression of F3'H and Overexpression of the Exogenous Senecio cruentus F3'5'H Gene

Cited by 79 publications

References 51 publications

Transcriptomics and Metabolite Analysis Reveals the Molecular Mechanism of Anthocyanin Biosynthesis Branch Pathway in Different Senecio cruentus Cultivars

Transcriptomics and Metabolite Analysis Reveals the Molecular Mechanism of Anthocyanin Biosynthesis Branch Pathway in Different Senecio cruentus Cultivars

The molecular basis for an ancient colour mutant in sweet pea (Lathyrus odoratus)

Evolution and developmental genetics of floral display—A review of progress

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