Flower endophytic fungi of Geodorum densiflorum endangered orchid

Abstract

Geodorum densiflorum is an ornamental and medicinally important orchid. The medicinal metabolites are produced by endophytic fungi associated with orchid tissue, particularly flowers. However, there is no report of the endophytic fungi from the G. densiflorum flowers. This research aimed to study the endophytic fungi from different parts of G. densiflorum flower. Fungal isolation was carried out from sepal, petal, labium, stigma, and anther using surface sterilization method. The fungi were identified by combining morphological and molecular characteristics of ITS rDNA region. The results showed that each flower organ had different species of endophytic fungi. A total of seven species of endophytic fungi were obtained; four species were successfully identified by molecular identification and three species based on morphology. The four species, namely Hypomontagnella barbarensis, Aspergillus oryzae, Curvularia pseudobranchyspora, and Nigrospora chinensis, while the three species, namely Gonatobotrys sp., Humicola sp., Aspergillus section Nigri. The labium inhabited by Curvularia pseudobranchyspora, Nigrospora chinensis, Aspergillus section Nigri. Three species isolated from petals, namely Hypomontagnella barbarensis, Gonatobotrys sp., and Aspergillus oryzae. The sepal is inhabited by Humicola sp. There were no endophytic fungi in stigma and anther. This indicates that each flower part is a unique habitat of endophytic fungi.

Diversity of unique, nonmycorrhizal endophytic fungi in cultivated Phalaenopsis orchids: A pilot study

“…Lecythophora produces lecythomycin, a putative antimicrobial compound that is believed to protect the host plant from pathogen attack (Sugijanto et al, 2009(Sugijanto et al, , 2011. Similarly, species of Hypomontagnella have been found to produce antifungal polyketides (Rahayu et al, 2021). Fusarium species, which were found in three of the five plants tested, are known to produce an abundance of compounds with diverse biological effects across a range of plant species (Bilal et al, 2018).…”

Isolasi dan Identifikasi Cendawan Endofit Akar Anggrek Epifit dan Hemiepifit

“…Anggrek memiliki keanekaragaman cendawan yang tinggi. Rahayu et al (2021) melaporkan bahwa organ bunga anggrek tanah Geodorum densiflorum asal Sulawesi dikolonisasi oleh cendawan Aspergillus section Nigri, A. oryzae, Curvularia pseudobranchyspora, Humicola sp., Hypomontagnella barbarensis, dan Nigrospora chinensis. Selain itu, Sukarno et al (2023) juga melaporkan bahwa terdapat cendawan endofit Fusarium solani species complex (FSSC) pada empat spesies anggrek, yaitu anggrek epifit Cymbidium finlaysonianum Lindl.…”