Fluconazole Susceptibility and Genotypic Heterogeneity of Oral Candida albicans Colonies from the Patients with Cancer Receiving Chemotherapy in China

Sun, Jing; Cheng, Quan; Lafleur, Micheal D; Qi, Qing-guo

doi:10.4248/ijos.09020

Cited by 13 publications

(10 citation statements)

References 28 publications

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“…Fothergill (2012) [43] recorded a lower range of the MIC of fluconazole (≤0.125-64 μg/ml ) against clinical C. albicans isolates from 2000-2009. However, a relatively similar observation was recorded by Sun and his colleagues [44] in which approximately 70% of C. albicans starins isolated from the oral cavity of cancer patients under chemotherapy were resistant to fluconazole and the MIC of all the resistant isolates was >128 μg/ml. He has attributed this great variation in fluconazole susceptibility to the genotypic heterogeneity among C. albicans isolates.…”

Section: Discussionsupporting

confidence: 76%

Oral <i>Candida</i> in β-thalassemia Major and Healthy Population and Their Fluconazole Susceptibility Pattern

Abdulaziz¹,

Muhammad²

2014

IJDSR

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Objective: Candida constitutes the main yeast flora in the oral cavity. Different species are known with C. albicans being the principal one. In healthy individuals, the balance among the oral microbial flora is maintained. However, this equilibrium might be altered in certain diseases resulting in the predominance of yeast flora over other microorganisms and behave as pathogens. Thalassemias constitute a large group of immunocompromised patients in our country with multiple hematological, immunological and endocrine disorders making them susceptible to local and systemic opportunistic yeast infections. Fluconazole is a globally used antifungal antibiotic especially in treating recurrent infections in immunocompromised patients. Little is known about the oral yeast carriage in the healthy individuals and thalassemic patients in our population, and their susceptibility pattern to fluconazole. This study has been done to assess the frequency of oral Candida carriage in a group of β-thalassemia major patients and sex and age-matched healthy controls, and to evaluate the susceptibility pattern of the isolates to fluconazole.

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Section: Discussionsupporting

confidence: 76%

Oral <i>Candida</i> in β-thalassemia Major and Healthy Population and Their Fluconazole Susceptibility Pattern

Abdulaziz¹,

Muhammad²

2014

IJDSR

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“…The results presented here and some controversial data from the literature (Sun et al, 2009) suggest that genetic diversity assessed by RAPD-PCR of C. albicans strains isolated from the upper respiratory tract or oral cavity, including those with dental infections seems to be dependent not only on the type of primer used but also may be related to origin of the strains from different geographical areas, e.g. Europe or Asia.…”

Section: Discussionmentioning

confidence: 70%

“…In our study primer RSD12 was employed. Primers, such as RSD6, RSD10 or RSD11, were also used separately or in combination by other authors (Waltimo et al, 2001;Samaranayake et al, 2003;Teanpaisan et al, 2008;Sun et al, 2009;Szweda et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Anticancer treatment including chemotherapy and/or radiotherapy is still associated with several long-term adverse effects. Among them, oral candidiasis is one of the major sources of illness, although strict oral hygienic care by using antifungal reagents is being employed to prevent it (Teanpaisan et al, 2008;Sun et al, 2009;Lee et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

et al. 2014

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Cancer patients are predisposed to fungal infections caused by Candida albicans, especially to oral or respiratory tract candidiasis. The aim of this study was to estimate genetic diversity by RAPD-PCR (random amplified polymorphic DNA-polymerase chain reaction) of C. albicans isolated from upper respiratory tract of 100 patients with non-small cell lung cancer. Among 52 strains, 34 genotypes were defined. 10 clusters comprising 28 (53.85%) isolates with similarity coefficient ≥ 80% were formed. The remaining 24 (46.15%) isolates represented individual genotypes. The RAPD-PCR technique revealed genomic variability within C. albicans isolated from upper respiratory tract of the cancer patients.

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“…Emergent resistant populations of Candida spp. treated with antifungals have been frequently reported, demonstrating the need to determine the levels of susceptibility of clinical isolates (1,2). Invasive fungal infections are important infections and their prevalence is increasing in immunocompromised patients, such as oncologic patients (3).…”

Section: Introductionmentioning

confidence: 99%

Antifungal Susceptibility In Vitro Determined by the Etest(r) for Candida Obtained from the Oral Cavity of Irradiated and Elderly Individuals

et al. 2015

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This study aimed to evaluate the in vitro antifungal susceptibility of Candida species of head-and-neck-irradiated patients (Group 1), non-institutionalized (Group 2) and institutionalized elders (Group 3) using Etest® methodology. Candida was isolated from saliva and presumptively identified by CHROMagar Candida(r), confirmed by morphological criteria, carbohydrate assimilation (API 20C AUX®) and genetic typing (OPE 18). The collection was made from 29, 34 and 29 individuals (Groups 1, 2 and 3, respectively) with 67 isolates. Etest® strips (ketoconazole, itraconazole, fluconazole, amphotericin B and flucytosine) on RPMI (Roswell Park Memorial Institute) agar, on duplicate, were used to evaluate susceptibility. ATTC (American Type Culture Collection) 10231 (Candida albicans) was used as quality control. Among the 67 isolates of Candida species, most were susceptible to azoles, flucytosine and amphotericin B. None of the isolates showed resistance and dose-dependent susceptibility to amphotericin B. There were nine strains resistant to itraconazole, six to fluconazole and two to ketoconazole and ten dose-dependent, mainly to flucytocine. The highest MIC (minimum inhibitory concentration) to C. albicans, C. tropicalis, C. parapsilosis was 2.671 μg.mL-1, 8.104 μg.mL-1, 4.429 μg.mL-1, all for flucytosine. C. krusei and C. glabrata were associated with higher MIC for azoles and C. glabrata with higher MIC to flucytosine. In summary, susceptibility to all tested antifungal agents was evident. The isolates were more resistant to itraconazole and dose-dependent to flucytosine. A comparison of C. albicans in the three groups showed no outliers. Higher MIC was associated with C. krusei and C. glabrata.

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Fluconazole Susceptibility and Genotypic Heterogeneity of Oral Candida albicans Colonies from the Patients with Cancer Receiving Chemotherapy in China

Abstract: Abstract

Cited by 13 publications

References 28 publications

Oral <i>Candida</i> in β-thalassemia Major and Healthy Population and Their Fluconazole Susceptibility Pattern

Oral <i>Candida</i> in β-thalassemia Major and Healthy Population and Their Fluconazole Susceptibility Pattern

Differentiation by random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) of Candida albicans isolated from upper respiratory tract in patients with non-small cell lung cancer.

Antifungal Susceptibility In Vitro Determined by the Etest(r) for Candida Obtained from the Oral Cavity of Irradiated and Elderly Individuals

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