Fluctuation of Peripheral Blood T, B, and NK Cells during a Menstrual Cycle of Normal Healthy Women

Lee, Sungki; Kim, Jeeyun; Jang, Byungwoo; Hur, Sungeun; Jung, Un Suk; Kil, Kihyun; Na, Baegju; Lee, Millina; Choi, Yearn–Ik; Fukui, Atsushi; Gilman‐Sachs, Alice; Kwak‐Kim, Joanne

doi:10.4049/jimmunol.0904192

Cited by 106 publications

(93 citation statements)

References 45 publications

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“…In this case, one could expect the increase in IFN-g production by female cells due to enhanced T h 1 responses due to low level of estrogen [8]. However, according to Lee et al, percentages of T H 1/T H 2 cytokine-producing T cells do not change significantly throughout cycle [36]. Faas et al [37] also have shown that intracellular levels of IFN-g, IL-2 or IL-10 do not vary between the phases of the ovarian cycle.…”

Section: Discussionmentioning

confidence: 93%

Influence of oxygen concentration on T cell proliferation and susceptibility to apoptosis in healthy men and women

Waskowska¹,

Lisowska

Daca³

et al. 2017

Folia Histochem Cytobiol.

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Introduction.Much of what we know about the functioning of human T lymphocytes is based on the experiments carried out at atmospheric oxygen (O 2 ) concentrations, which are significantly higher than those maintained in blood. Interestingly, the gender differences in the activity of T cells and their susceptibility to apoptosis under different O 2 conditions have not yet been described. The aim of the study was to compare two main markers of lymphocyte function: proliferation capacity and ability to produce cytokines as well as their susceptibility to apoptosis under two different O 2 concentrations, between men and women. Material and methods. 25 healthy volunteers, both males (13) and females (12) were recruited to the study (mean age 25.48 ± 5.51). By using cytometry proliferation parameters of human CD4 + CD28+ cells or CD8 + CD28 + cells in response to polyclonal stimulation of the TCR/CD3 complex at atmospheric (21%) and physiological (10%) O 2 concentrations using our modified dividing cell tracking technique (DCT) were analyzed as well as the percentages of apoptotic cells. We also determined the levels of IFN-g, IL-2, IL-10 and IL-17A using Cytometric Bead Array Flex system in cell culture supernatants. Results. CD4+ CD28 + and CD8 + CD28 + cells from the whole study group were characterized by shorter time required to enter the first (G1) phase of the first cell cycle at 21% compared to 10% O 2 . Both T cell populations performed significantly more divisions at 21% O 2 . The percentages of dividing cells were also significantly higher at atmospheric O 2 . Interestingly, data analysis by gender showed that male lymphocytes had similar proliferative parameters at both O 2 concentrations while female lymphocytes proliferate more efficiently at 21% oxygen. Compared to males, the female CD4 + cells showed increased susceptibility to apoptosis at both O 2 concentrations. No differences in the levels of cytokines regardless of gender and oxygen conditions were found. Conclusions. We showed that in vitro female T cells (both CD4 + and CD8 + cells) are more sensitive than male lymphocytes to low O 2 concentration as demonstrated by the decrease in their proliferation dynamics. The effect does not depend on increased apoptosis of female T cells under low O 2 because percentage of apoptotic cells was similar at both O

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Section: Discussionmentioning

confidence: 93%

Influence of oxygen concentration on T cell proliferation and susceptibility to apoptosis in healthy men and women

Waskowska¹,

Lisowska

Daca³

et al. 2017

Folia Histochem Cytobiol.

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“…On the other hand, the estradiol/progesterone balance during the menstrual cycle can mediate immunity and have an impact on the proportions of B and T lymphocytes [31,32]. It might also have an impact on their mitotic behaviour.…”

Section: Discussionmentioning

confidence: 99%

Possible influence of menstrual cycle on lymphocyte X chromosome mosaicism

Geršak

Perme-Pohar

Veble

et al. 2014

J Assist Reprod Genet

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Purpose Estrogens are known to selectively influence cell proliferation. Physiological variations of blood hormone concentration might play a role in regulating the level of X chromosome aneuploidy. In this study we observed the percentages of X aneuploid cells in standard lymphocyte cultures from blood samples obtained in relation to the menstrual cycle, noting whether collection occurred during either the follicular or the luteal phase. Methods A study consisting of 28 women with X mosaicism and recurrent pregnancy loss, and 28 age-matched healthy controls. Cytogenetic studies were carried out on peripheral blood samples according to standard procedures. Results A significant difference in the percentage of X aneuploidy was found in blood samples obtained during different phases of the menstrual cycle. In the case group, the mean value of aneuploid cells in the follicular and luteal phase samples was 10.0 and 6.3 % respectively and in the control group, it was 2.8 and 1.0 % (P<0.0001). The difference in the case group varied between 0 and 8 % (3.6±2.1 %) and in the control group between 0 and 4 % (1.7±1.1 %). The specificity for detecting true X mosaicism was 0.875. We estimate that the initial diagnosis of X mosaicism could be correct in 68 % of patients with recurrent pregnancy loss. Conclusions This observational study establishes that the time of blood sampling in relation to the menstrual cycle can influence lymphocyte X chromosome mosaicism. The results, further proven by additional controlled studies, would have practical implications for genetic counselling and fertility treatment.

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“…low statistical power of the study. In comparison to other studies on immunological fluctuations in relation to the menstrual cycle and use of CHC, earlier studies have both smaller (54,87) and larger (57) sample sizes.…”

Section: Immunological Assaysmentioning

confidence: 90%

“…Indeed, previous studies indicated estrogenic effect on T cell number during normal menstrual cycles (87) and during CHC cycles (93). Therefore, our approach of using CD3E…”

Section: Immunological Assaysmentioning

confidence: 96%

Multiple Sclerosis in relation to sex steroid exposure

Kempe¹

2014

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Fluctuation of Peripheral Blood T, B, and NK Cells during a Menstrual Cycle of Normal Healthy Women

Cited by 106 publications

References 45 publications

Influence of oxygen concentration on T cell proliferation and susceptibility to apoptosis in healthy men and women

Influence of oxygen concentration on T cell proliferation and susceptibility to apoptosis in healthy men and women

Possible influence of menstrual cycle on lymphocyte X chromosome mosaicism

Multiple Sclerosis in relation to sex steroid exposure

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