Fluctuations in total antioxidant capacity, catalase activity and hydrogen peroxide levels of follicular fluid during bovine folliculogenesis

Gupta, Sajal; Choi, Audrey H.; Yu, Hope Y.; Czerniak, Suzanne M.; Holick, Emily A.; Paolella, Louis J.; Agarwal, Ashok; Combelles, Catherine M.H.

doi:10.1071/rd10270

Cited by 57 publications

(39 citation statements)

References 34 publications

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“…Oxidative stress occurs when the generation of ROS exceeds the scavenging capacity of antioxidants, either due to excessive ROS production or an inadequate availability of antioxidants. At physiological levels, ROS is required for reproductive functions including oocyte maturation, folliculogenesis, ovarian steroidogenesis, luteolysis, ovulation and cyclical endometrial changes [2] . However ROS, higher than physiological levels, contribute to several pathological conditions, such as retained placenta, udder oedema, mastitis, infertility which in turn may impair reproductive performances [3] .…”

Section: Introductionmentioning

confidence: 99%

The Effect of Estrous Cycle on Oxidant and Antioxidant Parameters in Dairy Cows

Aydılek¹,

Varışlı²,

Selek³

et al. 2014

Kafkas Univ Vet Fak Derg

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Reactive oxygen species have fundamental roles in reproductive functions. To comprehensively evaluate the relation between reactive oxygen species and infertility, physiological variations across the estrous cycle in healthy cows have to be known. For this purpose 25 healthy multiparous Holstein dairy cows having regular estrous cycles were used. The estrous cycles were synchronized by ovsynch protocol. Oxidant [lipid hydroperoxide (LOOH), total oxidant status (TOS), oxidative stress index (OSI)], antioxidant parameters [total antioxidant status (TAS), total free sulfhydryl groups (SH), ceruloplasmin (CP), paraoxonase-1 (PON1), arylesterase (ARE), uric acid (UA)], lipid profile and progesterone levels were assayed at estrus, metestrus, diestrus and proestrus stages of the estrous cycle in the plasma samples. The plasma levels of oxidant (LOOH, TOS and OSI) and antioxidant (TAS, SH and UA) parameters were significantly decreased during the luteal phase compared to the follicular phase (at proestrus and at estrus) of the estrous cycle. There was also a significant positive correlation between TAS and TOS. The activity of PON1 and ARE significantly increased only at diestrus. Levels of high density lipoprotein, low density lipoprotein and total cholesterol elevated during the follicular phase (estrus) and declined during the luteal phase. In conclusion, oxidant/antioxidant status and lipid profile were affected by cyclic changes. Moreover, antioxidant defense system showed adaptive response to increased oxidative activities by occurring parallel increases and it may indicate that there is a dynamic balance between oxidant and antioxidant status during the estrous cycle in healthy cows. Keywords: Cow, Estrous cycle, Lipid peroxide, Lipid profile, Total antioxidant status, Total oxidant status Sütçü İneklerde Östrus Siklusunun Oksidan ve Antioksidan Parametreler Üzerine Etkisi ÖzetReaktif oksijen türlerinin üreme fonksiyonları üzerinde önemli rolleri vardır. Sağlıklı süt sığırlarında reaktif oksijen türleri ve infertilite arasındaki ilişkiyi kapsamlı bir şekilde değerlendirmek için östrus siklusu boyunca reaktif oksijen türü seviyelerindeki değişimlerin bilinmesi gerekir. Bu çalışmada düzenli siklus gösteren 25 adet sağlıklı Holştayn ırkı sütçü inek kullanıldı. Östrus siklusları ovsynch protokolü ile senkronize edildi. Östrus siklusunun proöstrus, östrus, metaöstrus ve diöstrus dönemlerinde kan plazmasında oksidan parametreler [lipit hidroperoksit (LOOH), total oksidan seviye (TOS), oksidatif stres indeksi (OSI)], antioksidan parametreler [total antioksidan kapasite (TAS), total serbest sülfhidril gruplar (SH), seruloplazmin (CP), paraoksonaz-1 (PON1), arilesteraz (ARE), ürik asit (UA)], lipit profili ve progesteron seviyesi ölçüldü. Foliküler dönemle karşılaştırıldığında luteal dönemdeki plazma oksidan (LOOH, TOS, OSI) ve antioksidan (TAS, SH, UA) seviyelerinin önemli derecede düştüğü görüldü. Ayrıca TAS ve TOS değerleri arasında önemli bir pozitif korelasyon vardı. Paraoksonaz-1 ve ARE aktiviteleri sadece diöstrus ...

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Section: Introductionmentioning

confidence: 99%

The Effect of Estrous Cycle on Oxidant and Antioxidant Parameters in Dairy Cows

Aydılek¹,

Varışlı²,

Selek³

et al. 2014

Kafkas Univ Vet Fak Derg

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“…Adicionalmente, é ainda possível que tenha ocorrido a exposição dos oócitos a fatores deletérios provenientes de folículos atrésicos, ou mesmo de maior diâmetro, já que durante a punção não era possível aferir de forma precisa o tamanho folicular. Outra característica relevante é a relação inversa entre a faixa etária e a concentração das enzimas envolvidas na desintoxicação de EROs, como a glutationa transferase, observada em humanos (28) e bovinos (29) . Como geralmente não se conhece a idade das vacas utilizadas nos distintos experimentos, é possível que este seja um importante fator responsável pelos resultados controversos em diferentes estudos sobre a composição e o efeito da utilização do LF durante a MIV.…”

Section: Resultsunclassified

CRIOTOLERÂNCIA DE OÓCITOS E EMBRIÕES BOVINOS MATURADOS COM LÍQUIDO FOLICULAR E/Ou Β-Mercaptoetanol

et al. 2015

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ResumoFoi avaliada a criotolerância de oócitos e embriões bovinos maturados com adição de líquido folicular (LF) e/ou β-mercaptoetanol (BM). Após vitrificação, os oócitos foram maturados em: TCM-199 (controle); BM (24h TCM-199+100µM BM); LF (6h em LF+18h TCM-199) e LF+BM (6h LF+18h TCM-199+100µM BM). Não houve diferença (p>0,05) nas taxas de blastocistos dos tratamentos TCM (6,4%), BM (4,0%) e LF (3,4%). A eclosão e densidade celular dos embriões eclodidos não diferiram (p>0,05) nos tratamentos. No Experimento 2 blastocistos expandidos (Bx) obtidos em D7 ou D8 foram vitrificados, avaliando-se sua reexpansão e eclosão. A reexpansão foi semelhante (p>0,05), sendo observado comportamento distinto na eclosão entre Bx D7 e D8. Nos Bx D7 houve maior eclosão no controle (TCM-54,2%) em relação ao BM (40,32%) e LF+BM (33,89%). Os Bx D8 apresentaram menor eclosão no controle (TCM) em relação aos Bx D7. Nos tratamentos BM, LF e LF+BM a eclosão foi semelhante para Bx D7 ou D8. A maturação com adição de LF e/ou BM não melhora a criotolerância de oócitos imaturos e embriões PIV. Blastocistos expandidos precoces (D7) são mais criotolerantes e apresentam um comportamento distinto à adição de LF e BM, em relação aos tardios (D8). Palavras-chave: aditivos; criotolerância; embrião PIV; maturação; vitrificação. AbstractCryotolerance of bovine oocytes and embryos maturated with addition of follicular fluid (LF) and β-

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“…Hennet and C.M.H. Combelles in bovine follicular fluid decrease with increasing follicle size (Combelles et al, 2010), although total antioxidant capacity (TAC) was reported to be highest in the follicular fluid of larger follicles (Gupta et al, 2011). These trends suggest that the antioxidant/ ROS balance in follicular fluid is actively and specifically adapted as folliculogenesis and oogenesis progress.…”

Section: Reactive Oxygen Species (Ros) and Antioxidantsmentioning

confidence: 93%

The antral follicle: a microenvironment for oocyte differentiation

Hennet

Combelles²

2012

Int. J. Dev. Biol.

Self Cite

152

119

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Mammalian reproduction hinges upon the timely ovulation of a fully differentiated oocyte. This event is the culmination of a complex and dynamic developmental relationship between the oocyte and the antral follicle housing it; the antral follicle constitutes a specialized microenvironment or niche, uniquely suited to the needs of the oocyte as it approaches ovulation. During this time, the oocyte must complete its final growth, capacitation, and nuclear and cytoplasmic maturation. Its microenvironment-the antral follicle-is in turn responsible for the integrity of these processes and the production of a high quality oocyte. Components of the antral follicle, including three distinct somatic cell types (theca, granulosa and cumulus), the basal lamina, and follicular fluid, each have active and regulatory roles in oocyte differentiation. Several milestones in antral folliculogenesis also have an influence on oocyte development. This review will discuss the antral follicle microenvironment with specific attention to its importance in oocyte differentiation. As assisted reproductive technologies (ART) often require stages of oocyte differentiation to occur in vitro rather than in vivo, current knowledge of the antral follicle microenvironment will also be discussed with respect to its clinical applications.

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Fluctuations in total antioxidant capacity, catalase activity and hydrogen peroxide levels of follicular fluid during bovine folliculogenesis

Cited by 57 publications

References 34 publications

The Effect of Estrous Cycle on Oxidant and Antioxidant Parameters in Dairy Cows

The Effect of Estrous Cycle on Oxidant and Antioxidant Parameters in Dairy Cows

CRIOTOLERÂNCIA DE OÓCITOS E EMBRIÕES BOVINOS MATURADOS COM LÍQUIDO FOLICULAR E/Ou Β-Mercaptoetanol

The antral follicle: a microenvironment for oocyte differentiation

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