Fluid Flow Dependency in Immunoselective Cell Capture via Liquid Biopsy

Abstract

Liquid biopsy targets rare cells that overexpress disease-specific membrane markers and capture these cells via immunoaffinity. The diagnosis efficiency of liquid biopsy can be impaired by the presence of healthy adherent cells also expressing the same biomarkers. Here, we investigated the effect of settling times and rinsing flow rates on the efficiency of EpCAM-based immunocapture using both simulation and experiments with three different cell types. Cell-surface adhesion forces and shear rates were calculated to define the range of rinsing flow rates to test experimentally. Healthy adherent cells did not adhere to blocked immunofunctionalized surfaces within the timeframe of the experiment; however, healthy EpCAM positive cells did bind to the surface to some extent. The greatest difference in capture efficiency was obtained using a high rinsing flow rate of 25 mL/min following 40 min static incubation, indicating that optimizing rinsing flow rates could be a viable option to capture, more specifically, cancer cells overexpressing EpCAM.

Paper-Supported Photoelectrochemical Biosensor for Dual-Mode miRNA-106a Assay: Integration of Luminescence-Confined Upconversion-Actuated Fluorescent Resonance Energy Transfer and CRISPR/Cas13a-Powered Cascade DNA Circuits

“…However, it involves many disadvantages, such as insufferable pain, unaffordable expense, and non-negligible infectivity. Liquid biopsies are a less wounded approach to monitor trace amounts of cancer biomolecules with lower expense, which just use serums and are patient-amiable, presenting great potential for high-efficiency diacrisis and prognosis of tumors . MicroRNA (miRNA) is the nonprotein-encoded ultrasmall molecular weight RNA nucleotide, which exists in the peripheric serum or plasma and expresses strong correlation with cancers. , Hence, it becomes a prevalent biomarker for liquid biopsies, but improvement is needed to detect exceedingly trace amounts of miRNAs and to avoid misdiagnosis .…”