Consider an aggregate arrival process A N obtained by multiplexing N On-Off processes with exponential Off periods of rate λ and subexponential On periods τ on . As N goes to infinity, with λN → Λ, A N approaches an M/G/∞ type process. Both for finite and infinite N , we obtain the asymptotic characterization of the arrival process activity period.Using these results we investigate a fluid queue with the limiting M/G/∞ arrival process A ∞ t and capacity c. When On periods are regularly varying (with non-integer exponent), we derive a precise asymptotic behavior of the queue length random variable Q P t observed at the beginning of the arrival process activity periodswhere ρ = EA ∞ t < c; r (c ≤ r) is the rate at which the fluid is arriving during an On period. The asymptotic (time average) queue distribution lower bound is obtained under more general assumptions on On periods than regular variation.In addition, we analyze a queueing system in which one On-Off process, whose On period belongs to a subclass of subexponential distributions, is multiplexed with independent exponential processes with aggregate expected rate Ee t . This system is shown to be asymptotically equivalent to the same queueing system with the exponential arrival processes being replaced by their total mean value Ee t .