Fluid Shear Stress Stimulates Prostaglandin and Nitric Oxide Release in Bone Marrow-Derived Preosteoclast-like Cells

McAllister, Todd; Du, Tianshu; Frangos, John A.

doi:10.1006/bbrc.2000.2467

Cited by 128 publications

(106 citation statements)

References 20 publications

Supporting

Mentioning

101

Contrasting

Order By: Relevance

“…Cbfa1 (Runx2) and osteocalcin are expressed in higher quantities by osteocytes than osteoblasts (Mikuni-Takagaki et al, 1995;Ducy et al, 1997) and might be responsible for the quicker www.ecmjournal.org E Birmingham et al Osteogenesis of MSCs in a simplifi ed bone niche osteogenic response in MSCs exposed to osteocyte factors. Certain pro-osteogenic signalling molecules, such as nitric oxide (NO) and prostaglandin E2 (PGE2), are released in large amounts from mechanically stimulated osteocytes in vitro (Klein-Nulend et al, 1995a;Klein-Nulend et al, 1995b;Keila et al, 2001), but are also expressed in static conditions (McAllister et al, 2000;Cheng et al, 2001). These signals could provide the stimuli for MSC osteogenic differentiation in vivo.…”

Section: Osteogenesis Of Mscs In a Simplifi Ed Bone Nichementioning

confidence: 99%

Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche

Birmingham

Niebur²,

McHugh³

et al. 2012

eCM

434

340

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) within their native environment of the stem cell niche in bone receive biochemical stimuli from surrounding cells. These stimuli likely infl uence how MSCs differentiate to become bone precursors. The ability of MSCs to undergo osteogenic differentiation is well established in vitro; however, the role of the natural cues from bone's regulatory cells, osteocytes and osteoblasts in regulating the osteogenic differentiation of MSCs in vivo are unclear. In this study we delineate the role of biochemical signalling from osteocytes and osteoblasts, using conditioned media and co-culture experiments, to understand how they direct osteogenic differentiation of MSCs. Furthermore, the synergistic relationship between osteocytes and osteoblasts is examined by transwell co-culturing of MSCs with both simultaneously. Osteogenic differentiation of MSCs was quantified by monitoring alkaline phosphatase (ALP) activity, calcium deposition and cell number. Intracellular ALP was found to peak earlier and there was greater calcium deposition when MSCs were co-cultured with osteocytes rather than osteoblasts, suggesting that osteocytes are more infl uential than osteoblasts in stimulating osteogenesis in MSCs. Osteoblasts initially stimulated an increase in the number of MSCs, but ultimately regulated MSC differentiation down the same pathway. Our novel coculture system confi rmed a synergistic relationship between osteocytes and osteoblasts in producing biochemical signals to stimulate the osteogenic differentiation of MSCs. This study provides important insights into the mechanisms at work within the native stem cell niche to stimulate osteogenic differentiation and outlines a possible role for the use of co-culture or conditioned media methodologies for tissue engineering applications.

show abstract

Section: Osteogenesis Of Mscs In a Simplifi Ed Bone Nichementioning

confidence: 99%

Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche

Birmingham

Niebur²,

McHugh³

et al. 2012

eCM

434

340

View full text Add to dashboard Cite

show abstract

“…This was until Davies et al 56) , in 1986, provided evidence that time-averaged shear stress alone could not explain the pathological behavior of endothelial cells exposed to complex flow patterns. Subsequent studies [57][58][59][60][61][62][63][64][65] have shown that vascular endothelial cells respond not only to time-averaged shear stress, but respond differently to different patterns of flow.…”

Section: Importance Of the Velocity Profilementioning

confidence: 99%

Use of Ultrasound for Non-Invasive Assessment of Flow-Mediated Dilation

Stoner

Sabatier

2012

JAT

View full text Add to dashboard Cite

The pathological complications of atherosclerosis, namely heart attacks and strokes, remain the leading cause of mortality in the Western world. Preceding atherosclerosis is endothelial dysfunction. There is therefore interest in the application of non-invasive clinical tools to assess endothelial function. The flow-mediated dilation (FMD) test is the standard tool used to assess endothelial function. Reduced FMD is an early marker of atherosclerosis and has been noted for its capacity to predict future cardiovascular disease events. This review discusses the measurement of endothelial function using ultrasound, with a focus on the FMD technique.

show abstract

“…In vitro studies have provided evidence that bone cells, both osteoblasts [Roclofsen et al, 1995;Stanford et al, 1995;Nagatomi et al, 2001] and osteoclasts [Rubin et al, 1999;McAllister et al, 2000;Kurata et al, 2001], directly respond to various kinds of mechanical stimuli. With regard to the effect of gravitational unloading, the studies have mainly focused on osteoblasts [Hughes-Fulford et al, 1998;Sarkar et al, 2000;Bikle et al, 2003;Ontiveros and McCabe, 2003], while few reports deal with the behavior of osteoclastic cells and their precursors.…”

mentioning

confidence: 99%

Modeled gravitational unloading triggers differentiation and apoptosis in preosteoclastic cells

et al. 2006

View full text Add to dashboard Cite

Gravity acts permanently on organisms as either static or dynamic stimulation. Understanding the influence of gravitational and mechanical stimuli on biological systems is an intriguing scientific problem. More than two decades of life science studies in low g, either real or modeled by clinostats, as well as experimentation with devices simulating different types of controlled mechanical stimuli, have shown that important biological functions are altered at the single cell level. Here, we show that the human leukemic line FLG 29.1, characterized as an osteoclastic precursor model, is directly sensitive to gravitational unloading, modeled by a random positioning machine (RPM). The phenotypic expression of cytoskeletal proteins, osteoclastic markers, and factors regulating apoptosis was investigated using histochemical and immunohistochemical methods, while the expression of the corresponding genes was analyzed using RT-PCR. A quantitative bone resorption assay was performed. Autofluorescence spectroscopy and imaging were applied to gain information on cell metabolism. The results show that modeled hypogravity may trigger both differentiation and apoptosis in FLG 29.1 cells. Indeed, when comparing RPM versus 1 Â g cultures, in the former we found cytoskeletal alterations and a marked increase in apoptosis, but the surviving cells showed an osteoclastic-like morphology, overexpression of osteoclastic markers and the ability to resorb bone. In particular, the overexpression of both RANK and its ligand RANKL, maintained even after return to 1 Â g conditions, is consistent with the firing of a differentiation process via a paracrine/autocrine mechanism. J. Cell. Biochem. 98: 65-80, 2006. ß 2005 Wiley-Liss, Inc.Key words: weightlessness; preosteoclastic differentiation; apoptosis; bone resorption; osteoporosis; gene expressionThe importance of gravity in modulating some biological processes, such as plant gravitropism and the adaptation of the vertebrate skeleton in relation to its load-bearing function, has long been known. Nevertheless, the role of gravity and other mechanical factors in biological processes is far from clear. A weightless environment is a powerful tool for understanding this role and for studying the related cellular and molecular mechanisms. Several investigations conducted in space laboratories and with instruments averaging the gravity vector, and thus simulating conditions of reduced gravity, have shown that isolated cells may change their behavior under altered gravitational conditions. Loss of cell activation [Cogoli et al., 1984], perturbation of signal transduction [Schmitt et al., 1996;Hashemi et al., 1999] and modification of genetic expression [Walther et al., 1998;Hammond et al., 1999] have been described. This suggests that some of the mechanisms at the roots of the systemic effects observed in weightlessness, such as depression of the immune system [Gmü nder and Cogoli, 1996]

show abstract

Fluid Shear Stress Stimulates Prostaglandin and Nitric Oxide Release in Bone Marrow-Derived Preosteoclast-like Cells

Cited by 128 publications

References 20 publications

Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche

Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche

Use of Ultrasound for Non-Invasive Assessment of Flow-Mediated Dilation

Modeled gravitational unloading triggers differentiation and apoptosis in preosteoclastic cells

Contact Info

Product

Resources

About