FluidFM as a tool to study adhesion forces of bacteria - Optimization of parameters and comparison to conventional bacterial probe Scanning Force Spectroscopy

Hofherr, Linda; Müller‐Renno, Christine; Ziegler, Christiane

doi:10.1371/journal.pone.0227395

Cited by 16 publications

(17 citation statements)

References 31 publications

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“…Even though diverse forces are applied to the bacterial cells and the geometry of cantilevers differs, the results of conventional force spectroscopy and FluidFM are comparable. 26 To ensure that only a single bacterium was measured, they were targeted individually under a microscope. Thus, efficient measurement of a comparable higher number of different bacterial cells (12 individual bacteria, each 16 times at different positions) in a native environment (e.g., in liquid) almost independently of their size, shape, and adhesion forces was possible.…”

Section: Resultsmentioning

confidence: 99%

Influence of the Available Surface Area and Cell Elasticity on Bacterial Adhesion Forces on Highly Ordered Silicon Nanopillars

et al. 2022

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Initial bacterial adhesion to solid surfaces is influenced by a multitude of different factors, e.g., roughness and stiffness, topography on the micro- and nanolevel, as well as chemical composition and wettability. Understanding the specific influences and possible interactive effects of all of these factors individually could lead to guidance on bacterial adhesion and prevention of unfavorable consequences like medically relevant biofilm formation. On this way, the aim of the present study was to identify the specific influence of the available surface area on the adhesion of clinically relevant bacterial strains with different membrane properties: Gram-positive Staphylococcus aureus and Gram-negative Aggregatibacter actinomycetemcomitans . As model surfaces, silicon nanopillar specimens with different spacings were fabricated using electron beam lithography and cryo-based reactive ion etching techniques. Characterization by scanning electron microscopy and contact angle measurement revealed almost defect-free highly ordered nanotopographies only varying in the available surface area. Bacterial adhesion forces to these specimens were quantified by means of single-cell force spectroscopy exploiting an atomic force microscope connected to a microfluidic setup (FluidFM). The nanotopographical features reduced bacterial adhesion strength by reducing the available surface area. In addition, the strain-specific interaction in detail depended on the bacterial cell’s elasticity and deformability as well. Analyzed by confocal laser scanning microscopy, the obtained results on bacterial adhesion forces could be linked to the subsequent biofilm formation on the different topographies. By combining two cutting-edge technologies, it could be demonstrated that the overall bacterial adhesion strength is influenced by both the simple physical interaction with the underlying nanotopography and its available surface area as well as the deformability of the cell.

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Section: Resultsmentioning

confidence: 99%

Influence of the Available Surface Area and Cell Elasticity on Bacterial Adhesion Forces on Highly Ordered Silicon Nanopillars

et al. 2022

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“… 4 To date, an optimal protocol for measuring platelet adhesion using FluidFM is still missing. Here, we optimized the most important parameters in force spectroscopy measurement, including contact time and the setpoint, that directly influence the magnitude of the measured adhesion forces 71 before actually carrying out the force spectroscopy measurements between platelets and the investigated surfaces. We identified the most suitable contact time of 5 s and a setpoint of 10 nN for the platelet-surface adhesion force measurement.…”

Section: Discussionmentioning

confidence: 99%

Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films

et al. 2021

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Platelet-surface interaction is of paramount importance in biomedical applications as well as in vitro studies. However, controlling platelet-surface activation is challenging and still requires more effort as they activate immediately when contacting with any nonphysiological surface. As hydrogels are highly biocompatible, in this study, we developed agarose and gelatin-based hydrogel films to inhibit platelet-surface adhesion. We found promising agarose films that exhibit higher surface wettability, better controlled-swelling properties, and greater stiffness compared to gelatin, resulting in a strong reduction of platelet adhesion. Mechanical properties and surface wettability of the hydrogel films were varied by adding magnetite (Fe 3 O 4 ) nanoparticles. While all of the films prevented platelet spreading, films formed by agarose and its nanocomposite repelled platelets and inhibited platelet adhesion and activation stronger than those of gelatin. Our results showed that platelet-surface activation is modulated by controlling the properties of the films underneath platelets and that the bioinert agarose can be potentially translated to the development of platelet storage and other medical applications.

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“…The results significantly revealed a novel aggregation mechanism of C. albicans, in which force-dependent amyloid core sequences in Als proteins play a dual role, that is, in formation of adhesin nanoclusters and in homophilic bonding between amyloid sequences on opposing cells. Recently, the optimization of measurement parameters (e.g., setpoint, z-speed, z-length, pause time, and relative underpressure) when applying FluidFM-based SCFS on bacterial cells has been investigated [96]. As an alternative way, the bead whose surface is coated with chemical molecules can be attached to the aperture of the microchanneled cantilever, which is then used to probe the interactions between individual microbial cells (immobilized on the substrate) and bead surface [97].…”

Section: Adhesion Of Microbial Cellsmentioning

confidence: 99%

FluidFM for single-cell biophysics

Liu

Zambelli

2021

Nano Res.

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Fluidic force microscopy (FluidFM), which combines atomic force microscopy (AFM) with microchanneled cantilevers connected to a pressure controller, is a technique allowing the realization of force-sensitive nanopipette under aqueous conditions. FluidFM has unique advantages in simultaneous three-dimensional manipulations and mechanical measurements of biological specimens at the micro-/nanoscale. Over the past decade, FluidFM has shown its potential in biophysical assays particularly in the investigations at single-cell level, offering novel possibilities for discovering the underlying mechanisms guiding life activities. Here, we review the utilization of FluidFM to address biomechanical and biophysical issues in the life sciences. Firstly, the fundamentals of FluidFM are represented. Subsequently, the applications of FluidFM for biophysics at single-cell level are surveyed from several facets, including single-cell manipulations, single-cell force spectroscopy, and single-cell electrophysiology. Finally, the challenges and perspectives for future progressions are provided.

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FluidFM as a tool to study adhesion forces of bacteria - Optimization of parameters and comparison to conventional bacterial probe Scanning Force Spectroscopy

Cited by 16 publications

References 31 publications

Influence of the Available Surface Area and Cell Elasticity on Bacterial Adhesion Forces on Highly Ordered Silicon Nanopillars

Influence of the Available Surface Area and Cell Elasticity on Bacterial Adhesion Forces on Highly Ordered Silicon Nanopillars

Controlling Surface-Induced Platelet Activation by Agarose and Gelatin-Based Hydrogel Films

FluidFM for single-cell biophysics

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