Fluorescein

Abstract

This feature focuses on a reagent chosen by a postgraduate, highlighting the uses and preparation of the reagent in current research spotlight Fluorescein Frank ThielbeerFrank Thielbeer was born in Magdeburg, Germany, and studied chemistry at the University of Leipzig (Germany) and Monash University in Melbourne (Australia) receiving his M.Sc. degree in 2008. In the following year he started his PhD with Prof. Mark Bradley at the University of Edinburgh. His research interests focus on polymer particles and their modifications for applications in chemical biology.

Comprehensive Characterization of an “Off/On” Rhodol‐Based Lysosomal Tracker for Orthogonal Cellular Analysis by Confocal Imaging

“…[5] However, since the envisaged use for such compounds was the specific invivo labeling of SNAP-tag and Halo-tag fusion proteins, as well as actin and tubulin, these dyes have never been tested as trackers for lysosomes. We have thus investigated in this direction and prepared two different LTs covering the missing wavelength emission range between 490 and 530 nm by using fluorescein [6] and the poorly represented rhodafluor derivatives that emit in the same wavelength range. [7][8][9][10] In addition, we have prepared the aforementioned rhodamine based LT. [11] These off/on LTs named RBCN (compound 2), [11] FlCN (compound 4) and RhoCN (compound 7) were used to realize a series of confocal microscopy experiments where the mapping of pre-osteoblastic MC3T3-E1 cells [12] was performed by using a combination of a mitochondrial tracker (MT, MitoTracker Red CM-H2XRos™) and DAPI (Dapi-Fluoromount-G™) used for coloring mitochondria and cell nuclei, respectively.…”

“…However, since the envisaged use for such compounds was the specific in‐vivo labeling of SNAP‐tag and Halo‐tag fusion proteins, as well as actin and tubulin, these dyes have never been tested as trackers for lysosomes. We have thus investigated in this direction and prepared two different LTs covering the missing wavelength emission range between 490 and 530 nm by using fluorescein [6] and the poorly represented rhodafluor derivatives that emit in the same wavelength range [7–10] . In addition, we have prepared the aforementioned rhodamine based LT [11] .…”

A phthalocyanine-fluorescein conjugate

“…22 Fluorescein is used for fluorescence-based applications. 23 As a fluorescence probe, it has been widely functionalized to allow versatile coupling to molecules for their labeling: oligonucleotides, 24 nanoparticles, 25 pH sensitive moieties, 26 etc. Bioorthogonal approaches have been developed as well.…”