Fluorescein isothiocyanate stability in different solvents

Abstract

Nutrient transport in seasonally ice-covered lakes is an important driver of spring algal blooms in eutrophic waters because phase changes during the ice growth process redistribute the nutrients. In this study, nutrient transport under static conditions was simulated using two ice thickness models in combination with an indoor freezing experiment under different segregation coefficient conditions for nutrients. A real-time prediction model for nutrient and pollutant concentrations in ice-covered lakes was established to explore the impact of the ice-on period in eutrophic shallow lakes. The results showed that the empirical degreeday model and the high-resolution thermodynamic snow and sea-ice model (HIGHTSI) could both be used to simulate lake ice thickness. The empirical degree-day model performed better at predicting the maximum ice thickness (measured thickness 0.22-0.55 m; simulated thickness 0.48 m), while the HIGHTSI model was more accurate when estimating the mean thickness (5-6 % error). When simulating ice growth, the HIGHTSI model considered more meteorological factors impacting ice cover ablation; hence, it performed better during the ablation stage relative to the empirical degree-day model. Two nondynamic nutrient transport models were developed by combining the segregation coefficient model and the ice thickness prediction model. The HIGHTSI nutrient transport model can be used to predict real-time changes in nutrient concentrations under ice cover, and the degree-day model can be used to predict changes in the lake water ecosystem.

Uptake Quantification of Antigen Carried by Nanoparticles and Its Impact on Carrier Adjuvanticity Evaluation

“…FITC, as a hydrophobic molecule, exhibits high fluorescence in organic media like PBS/DMSO. However, in aqueous environments like PBS, FITC is prone to quenching due to aggregation [ 37 ]. This observation aligns with the general rule in fluorophore calibration, which states that the fluorophore concentration calibration for fluorescence labeling-based antigen quantification should be conducted in the same medium as the sample.…”

High biocompatible FITC-conjugated silica nanoparticles for cell labeling in both in vitro and in vivo models

“…Encapsulating FITC molecules within the SiO 2 matrix offers enhanced and sustained fluorescence 7 . This encapsulation method also stabilizes FITC against pH-related variations and shields it from photobleaching 13 , 14 .…”