Fluorescence contrast improvement by polarization shaped laser pulses for autofluorescent biomolecules

Abstract

We present contrast enhancement for the autofluorescing coenzymes flavin adenine dinucleotide (FAD) and nicotinamide adenine dinucleotide (NADH) in glycerol using phase and polarization shaped laser pulses after the transmission through a kagome fiber. Thereto, we report a way to calculate the optimal light modulator incident polarization angle, which in general differs from the horizontal. Combining phase and polarization shaping, we can selectively excite FAD in one polarization and simultaneously NADH in the other polarization direction by third order phase functions. Due to high anisotropy, the contrast of the fluorescence depends on the polarization direction. The effect of the fiber on the phase is precompensated in order to obtain the desired phase function after the fiber. Since the relative amounts of NADH and FAD give information about cellular metabolic activity which in turn helps understand disease processes, the method promises high biophotonic potential.

Inhibitive Mechanism of Loquat Flower Isolate on Tyrosinase Activity and Melanin Synthesis in Mouse Melanoma B16 Cells

“…Try's fluorescence intensity is higher than Phe's because it has one more conjugated double bond than Phe. Moreover, Try's fluorescence spectrum may even cover Phe's because the two fluorescence spectra overlap (Haas, Hild, Kussicke, Kurre, & Lindinger, 2020). According to this phenomenon, when tyrosinase is under fluorescence spectrophotometer, there is a fluorescence peak of its own, which will rise or fall depending on whether the inhibitor added interacts with it or not.…”