Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity

Savić, Aleksandar; Mitrović, Aleksandra; Donaldson, Lloyd; Radosavljević, Jasna Simonović; Pristov, Jelena Bogdanović; Steinbach, Gábor; Garab, Győző; Radotić, Ksenija

doi:10.1017/s143192761600009x

Cited by 8 publications

(4 citation statements)

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“…As a further use of quantitative FDLD imaging ( Figure 7 ), the response to mechanical stress was analysed on wood cell walls of juvenile Serbian spruce [ 53 ]. FDLD microscopy was used to compare the distribution and alignment of cellulose fibrils in cell walls of compression (CW) and normal wood (NW), on stem cross-sections of juvenile Picea omorika trees.…”

Section: Applications In Plant Cell Researchmentioning

confidence: 99%

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Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts

Radosavljević

Mitrović

Radotić

et al. 2021

IJMS

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Modern light microscopy imaging techniques have substantially advanced our knowledge about the ultrastructure of plant cells and their organelles. Laser-scanning microscopy and digital light microscopy imaging techniques, in general—in addition to their high sensitivity, fast data acquisition, and great versatility of 2D–4D image analyses—also opened the technical possibilities to combine microscopy imaging with spectroscopic measurements. In this review, we focus our attention on differential polarization (DP) imaging techniques and on their applications on plant cell walls and chloroplasts, and show how these techniques provided unique and quantitative information on the anisotropic molecular organization of plant cell constituents: (i) We briefly describe how laser-scanning microscopes (LSMs) and the enhanced-resolution Re-scan Confocal Microscope (RCM of Confocal.nl Ltd. Amsterdam, Netherlands) can be equipped with DP attachments—making them capable of measuring different polarization spectroscopy parameters, parallel with the ‘conventional’ intensity imaging. (ii) We show examples of different faces of the strong anisotropic molecular organization of chloroplast thylakoid membranes. (iii) We illustrate the use of DP imaging of cell walls from a variety of wood samples and demonstrate the use of quantitative analysis. (iv) Finally, we outline the perspectives of further technical developments of micro-spectropolarimetry imaging and its use in plant cell studies.

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Section: Applications In Plant Cell Researchmentioning

confidence: 99%

“…The blue and yellow colours of the co-ordinate axes correspond to the orientation of tangential and radial walls in the images, respectively. (Published in [ 53 ], with permission from Cambridge University Press).…”

Section: Figurementioning

confidence: 99%

Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts

Radosavljević

Mitrović

Radotić

et al. 2021

IJMS

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“…This is because most of the conventional methods for MFA measurement suffer from their low spatial resolution and vulnerability to various kinds of variations in the MFA, inside and between the tracheids (Donaldson 2008). However, techniques of synchrotron X-ray (Lichtenegger et al 1999), polarization (Abraham and Elbaum 2012;Mannan et al 2016;Savić et al 2016) and polarized Raman microscopy (Gielringer et al 2010) have a potential to overcome these shortcomings and gives us many insights through the visualization of MFA spatial distributions in a manner of a two-dimensional (2D) image. These methods enable us to directly quantify many anatomical features, such as multi-layered and pit structures, and provide a new basis for analyzing species-specific features.…”

Section: Introductionmentioning

confidence: 99%

Wood identification of two anatomically similar Cupressaceae species based on two-dimensional microfibril angle mapping

Kita

Sugiyama

2020

Holzforschung

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Identifying two anatomically similar species of Cupressaceae, Chamaecyparis obtusa and Thujopsis spp., is important to better understand the culture of wood use in Japan. However, the conventional method, which involves observing their cross-field pitting, cannot identify them in many cases. This study solves the above problem by introducing an anatomical criterion based on the micro fibril angle (MFA). MFA values were obtained through two-dimensional MFA images using the uniaxial optical anisotropy of cellulose microfibrils. A combination of the preprocessed MFA images and a convolutional neural network (CNN) yielded an accuracy nearly of 90% in classifying these species in cases of present and old wood specimens. Our feature extraction and classification techniques provide a new way for describing the anatomical features of wood and identifying featureless softwoods. Using the model interpretation-related methodologies of the CNN, distinct features of the two wood species were partly explained by MFA anisotropy in the S2 wall induced by the existence of pits.

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“…Previous studies performed by rotated samples and sequential imaging (Verbelen and Kerstens 2000; Kerstens and Verbelen 2003) and using the first-generation DP-LSM technique (Steinbach et al 2008), proved the anisotropic organization of the cellulose macromolecules in the cell wall. Further works investigated the differences in FDLD between different plant materials (hard wood, soft wood and maize) and environmental conditions, and mechanobiological aspects (Djikanović et al 2016; Savić et al 2016; Radosavljević et al 2017). The results of these works provided new data for comparison of the cell wall properties that may be important for the selection of appropriate plant and growth conditions for possible applications as a source of biomass.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence-detected linear dichroism imaging in a re-scan confocal microscope equipped with differential polarization attachment

et al. 2019

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Confocal laser scanning microscopy is probably the most widely used and one of the most powerful techniques in basic biology, medicine and material sciences that is employed to elucidate the architecture of complex cellular structures and molecular macro-assemblies. It has recently been shown that the information content, signal-to-noise ratio and resolution of such microscopes (LSMs) can be improved significantly by adding different attachments or modifying their design, while retaining their user-friendly features and relatively moderate costs. Differential polarization (DP) attachments, using high-frequency modulation/demodulation circuits, have made LSMs capable of high-precision 2D and 3D mapping of the anisotropy of microscopic samples—without interfering with their ‘conventional’ fluorescence or transmission imaging (Steinbach et al. in Methods Appl Fluoresc 2:015005, 2014 ). The resolution and the quality of fluorescence imaging have been enhanced in the recently constructed Re-scan confocal microscopy (RCM) (De Luca et al. in Biomed Opt Express 4:2644–2656, 2013 ). In this work, we developed the RCM technique further, by adding a DP-attachment modulating the exciting laser beam via a liquid crystal (LC) retarder synchronized with the data acquisition system; by this means, and with the aid of a software, fluorescence-detected linear dichroism (FDLD), characteristic of the anisotropic molecular organization of the sample, could be recorded in parallel with the confocal fluorescence imaging. For demonstration, we show FDLD images of a plant cell wall ( Ginkgo biloba ) stained with Etzold’s staining solution. Electronic supplementary material The online version of this article (10.1007/s00249-019-01365-4) contains supplementary material, which is available to authorized users.

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Fluorescence-Detected Linear Dichroism of Wood Cell Walls in Juvenile Serbian Spruce: Estimation of Compression Wood Severity

Cited by 8 publications

References 20 publications

Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts

Differential Polarization Imaging of Plant Cells. Mapping the Anisotropy of Cell Walls and Chloroplasts

Wood identification of two anatomically similar Cupressaceae species based on two-dimensional microfibril angle mapping

Fluorescence-detected linear dichroism imaging in a re-scan confocal microscope equipped with differential polarization attachment

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