Fluorescence Determination of Azithromycin in Pharmaceutical Formulations by Using the Synchronous Scanning Approach After its Acid Derivatization

Almeida, Vanessa G. K.; Braga, Victor S. M.; Pacheco, Wagner F.; Cassella, Ricardo J.

doi:10.1007/s10895-012-1111-8

Cited by 11 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, excessive use can lead to resistance, negatively impacting infection management. Furthermore, AZM has been detected in aquatic environments at high concentrations, requiring the development of more accurate and efficient analytical methods, [5] including high‐performance liquid chromatography (HPLC), [6] gas chromatography with mass spectroscopy (GC‐MS), [7] colorimetry, [8] spectrofluorometer, [9] and UV‐Vis spectroscopy [10] . These methods have drawbacks such as high cost, lengthy analysis time, and complex sample preparation process [11] .…”

Section: Introductionmentioning

confidence: 99%

A New Electrochemical Sensor for the Detection of Azithromycin using Screen‐Printed Carbon Electrode Modified with Boron‐Doped Diamond Nanoparticles and Reduced‐Graphene Oxide

Jiwanti,

Insani,

Sari

et al. 2024

ChemistrySelect

View full text Add to dashboard Cite

Excessive use of azithromycin (AZM) is associated with resistance and negative impacts on the environment. Therefore, this study aimed to develop an electrochemical sensor for AZM. The screen‐printed carbon electrode (SPCE) modified with boron‐doped diamond nanoparticle (BDDNP) and reduced‐graphene oxide (rGO) was successfully applied to measure the AZM concentration. The morphology of the modified electrode was characterized by scanning electron microscopy‐energy dispersive X‐ray (SEM‐EDX). Furthermore, the effect of electrochemical parameters, such as signal per background, scan rate, and selectivity was determined. The results showed that under optimum conditions, the plot of AZM demonstrated linearity in the range of 30–100 μM with a limit of detection (LOD) at 1.6 μM. The repeatability and recovery were also investigated, and the sensor was applied to real samples obtained from hospital wastewater. A remarkable 93.27 % recovery rate and excellent precision were recorded with an %RSD of 2.41 %. This study underscores the potential of SPCE modified with rGO/BDDNP for accurate and reliable measurement of AZM levels, showcasing great applicability in addressing the challenges associated with monitoring AZM in various environmental settings.

show abstract

Section: Introductionmentioning

confidence: 99%

A New Electrochemical Sensor for the Detection of Azithromycin using Screen‐Printed Carbon Electrode Modified with Boron‐Doped Diamond Nanoparticles and Reduced‐Graphene Oxide

Jiwanti,

Insani,

Sari

et al. 2024

ChemistrySelect

View full text Add to dashboard Cite

show abstract

“…As for AZT, it has been determined by high performance liquid chromatography (HPLC) [3][4][5][6], liquid chromatography-mass detection (LC-MS/MS) [7,8], ultra-performance liquid chromatography (UPLC) [9], micellar liquid chromatography (MLC) [10], spectroflourimetry [11] and UVVisible spectrophotometry [12][13][14][15][16][17] while ROX has been estimated by HPLC [18,19], LC-MS/MS [20], spectroflourimetry [21,22] and UV-Visible spectrophotometry [23].…”

Section: Introductionmentioning

confidence: 99%

Two spectrophotometric methods for the determination of azithromycin and roxithromycin in pharmaceutical preparations

2017

View full text Add to dashboard Cite

Two new and simple spectrophotometric procedures have been proposed and validated for estimation of two important macrolide antibiotics namely, azithromycin dihydrate and roxithromycin. Method I depends on complex formation between any of the two drugs and copper in acidic medium where the absorbances of the produced complexes are measured at 250 and 264 nm with linearity ranges of 1.0-100.0 and 2.0-130.0 µg/mL for the two drugs, respectively. Method II depends on the reaction of these drugs with N-bromosuccinimide forming a product which is yellow colored, measured at 264 and 278 nm, with linearity ranges of 2.0-140.0 and 3.0-160.0 µg/mL for azithromycin dihydrate and roxithromycin, respectively. The proposed methods were subjected to detailed validation procedure; moreover they were used for the estimation of the concerned drugs in their different dosage forms. Study of the reactions stoichiometry was carried out; furthermore, a reaction mechanism proposal was presented.

show abstract

“…1). Literature reveals few spectrophotometric methods [18][19][20][21][22][23] and two fluorimetric assays [24,25] for determination of AZN in bulk and formulations. A detailed review for RSGL estimation [26] is reported, without direct fluorimetric technique.…”

mentioning

confidence: 99%

Carbon dots as fluorescent nanoprobes for assay of some non-fluorophoric nitrogenous compounds of high pharmaceutical interest

Moustafa

Talaat

Youssef

et al. 2023

Beni-Suef Univ J Basic Appl Sci

View full text Add to dashboard Cite

Background Carbon dots, CDs, have excellent photoluminescence properties, good biocompatibility, low toxicity and good light stability. The optical, magnetic and electronic properties of CDs make them a hugely relevant tool to be used in pharmaceutical analysis, bioimaging, drug delivery, and other fields. The fluorescence of carbon nanodots makes it suitable for assay of some nitrogenous compounds of high pharmaceutical interest. In this work, we develop simple, fast and green spectrophotometric methods for quantification of Azithromycin and Rasagiline mesilate using synthesized fluorescent CDs from garlic peels. Results The spectrometric methods depend on stoichiometric reactions of both drugs with fluorescent CDs. Carbon dots exhibit a declared absorption peak λmax at 238 nm and potent fluorimetric emission at λem 528 nm, upon excitation at λex 376 nm. Drugs’ concentrations in ppm are efficiently calculated using Stern–Volmer Equation. Decrease in fluorescence (ΔF = Fo − F) and the F-ratio values are linearly correlated to molar concentration of each quencher (drug). A significant linear diminish in the dots’ measured absorbance and fluorimetric emission values was observed. Validation of all the developed methods was according to the ICH guidelines. Conclusions In a new way, this work successfully indicates, spectrometric methods for rapid detection of two non-fluorophoric nitrogenous compounds using potent carbon nanodots. Consequently, these green developed methods offer several benefits as simplicity, ease of quantification, accuracy and precision that encourage the application of the developed methods in routine analysis of Azithromycin and Rasagiline mesilate in quality control laboratories as analytical tool.

show abstract

Fluorescence Determination of Azithromycin in Pharmaceutical Formulations by Using the Synchronous Scanning Approach After its Acid Derivatization

Cited by 11 publications

References 25 publications

A New Electrochemical Sensor for the Detection of Azithromycin using Screen‐Printed Carbon Electrode Modified with Boron‐Doped Diamond Nanoparticles and Reduced‐Graphene Oxide

A New Electrochemical Sensor for the Detection of Azithromycin using Screen‐Printed Carbon Electrode Modified with Boron‐Doped Diamond Nanoparticles and Reduced‐Graphene Oxide

Two spectrophotometric methods for the determination of azithromycin and roxithromycin in pharmaceutical preparations

Carbon dots as fluorescent nanoprobes for assay of some non-fluorophoric nitrogenous compounds of high pharmaceutical interest

Contact Info

Product

Resources

About