Fluorescence Diagnosis of Cancer

Ae, Profio; Oj, Balchum

doi:10.1007/978-1-4613-2165-1_6

Cited by 36 publications

(13 citation statements)

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“…The observation that some porphyrins and their analogues are accumulated in significant amounts and retained for prolonged periods of time by a variety of solid tumours (Zhou, 1989;Marcus, 1992) has opened new prospects for the therapy of neoplastic lesions by taking advantage of the photosensitising properties of many tetrapyrrolic compounds (Jori & Spikes, 1984), as well as for the early diagnosis of such lesions based on the fluorescence emission typical of the porphyrin chromophore (Profio & Balchum, 1985;Unsold et al, 1990). The latter application is presently limited by two main factors: (i) the degree of selectivity of tumour labelling by the porphyrin is limited, because the ratio of photosensitiser concentration in the tumour to the peritumoral tissue often does not exceed 2-3:1 ; and (ii) the persistence of appreciable levels of photosensitivity in several tissues, including skin, for several weeks after systemic administration of the porphyrin (Dougherty, 1987).…”

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confidence: 99%

Carotenoporphyrins as selective photodiagnostic agents for tumours

Reddi

Segalla²,

Jori³

et al. 1994

Br J Cancer

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Summary The covalent binding of a carotene moiety to one phenyl ring and meso-tetraphenyl-substituted porphyrins (see Figure 1) efficiently quenches the photosensitising activity of the porphyrin while a relatively large yield of fluorescence emission around 650 nm is retained. Pharmacokinetic studies performed with two carotenoporphyrins (CPs) and the corresponding porphyrins (Ps) in Balb/c mice bearing an MS-2 fibrosarcoma show that the two Ps give a high selectivity of tumour localisation (tumour/peritumoral tissue ratios of dye concentration ranging between c. 30 and 90 at 24 h after injection of 4.2 -8.4 1tmol kg-in a Cremophor emulsion) and photosensitise tumour necrosis upon red light irradiation. For the same injected doses, the two CPs show no tumour-photosensitising activity even though they localise in the tumour in concentrations of the order of 10-40 jug g-' at 24 h with tumour/peritumoral ratios larger than 10. Thus, the fluorescence emitted by these CPs in the tumour can be used for photodiagnostic purposes with no risk of skin photosensitisation. However, this approach is presently limited by the large accumulation and prolonged retention of the CPs in the liver and spleen.

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confidence: 99%

Carotenoporphyrins as selective photodiagnostic agents for tumours

Reddi

Segalla²,

Jori³

et al. 1994

Br J Cancer

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“…Also a spectrum from the hpid contents of the same fatty plaque is shown (e). Finally, a spectrum from the surface of a non-diseased artery wall in given (1). The excitation wavelength was 337 nm.…”

Section: Introductionmentioning

confidence: 99%

<title>Fluorescence characteristics of atherosclerotic plaque and malignant tumors</title>

Andersson‐Engels¹,

Baert

Berg³

et al. 1991

SPIE Proceedings

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Two series of investigations utilizing laser-induced fluorescence (LIF) in characterizing diseased tissue are presented. In one in vitro investigation we studied the fluorescence from normal and atheroscierotically diseased arteries. In another, clinical, study we investigated the fluorescence in vivo from superficial urinary bladder malignancies in patients who had received a low-dose injection of Hematoporphyrin Derivative (HpD). Additionally, the fluorescence properties of L-tryptophan, collagen-I powder, elastin powder, nicotinamide adenine dinucleotide and i3-carothene were investigated and compared with the spectra from the tissue samples. A nitrogen laser (337 nm) alone or in connection with a dye laser (405 urn) was used together with an optical multichannel analyzer (OMA) to study the fluorescence spectra. The fluorescence decay characteristics of atherosclerotic plaque were examined utilizing a mode locked argon ion laser, synchronously pumping a picosecond dye laser. A fast detection system based on photon counting was employed. The fluorescence decay curves were evaluated on a PC computer allowing up to three lifetime components to be determined. A fluorescence peak at 390 nm in fibrotic plaque was identified as due to collagen fibers, while a fluorescence peak at 520 nm was connected to i3-carothene. The in vivo measurements of urinary bladder malignancies were performed with the optical fiber of the OMA system inserted through the biopsy channel of a cystoscope during the diagnostical procedure. The spectral recordings from urinary bladders, obtained at 337 nm and 405 nm excitation, revealed fluorescence features which can be used to demarcate tumor areas from normal mucosa. The fluorescence emission might also be useful to characterize different degrees of dysplasia.

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“…As already pointed out, the autofluorescence in the region peaking at about 470 nm decreases in malignant tissue at the same time as the specific fluorescence signal in the red wavelength region increases. By If the fluorescence signal in the red wavelength region is lifted off the background autofluorescence signal, on which it is superimposed, the contrast is enhanced [1,[4][5][6][34][35][36]. This is illustrated in Fig.…”

Section: Contrast Enhancementmentioning

confidence: 99%

“…In some types of human malignancies, if not found although known to be present, no alternative diagnostic tool is available until the tumor has grown large enough to be visualized. Laser-induced fluorescence (LIF) as an additional diagnostic tool may improve the probability of earlier discovery [1]. The method relies on the fact that different tissue Apart from the diagnosis of malignant tumors LIF may be incorporated for guiding in the procedure of laser angioplasty, a field in which a remarkable development has ocurred during the last few years.…”

Section: Introductionmentioning

confidence: 99%