Fluorescence energy transfer inhibition bioassay for cholera toxin based on galactose-stabilized gold nanoparticles and amine-terminated quantum dots

Ahn, Kwang Soo; Lim, Kyung Rae; Jeong, Daeho; Lee, Bo Young; Kim, Kwan Soo; Lee, Won Yong

doi:10.1016/j.microc.2015.07.007

Cited by 24 publications

(10 citation statements)

References 35 publications

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“…In the intestines, the bacteria colonize the mucosa and begin to secrete cholera toxin (CT), which initiates the disease symptoms [ 127 ]. Nanosensors have been fabricated to detect both Vibrio cholerae [ 128 , 129 ] and CT, but the majority of the literature has focused on detection of CT subunit B (CT-B) [ 130 – 134 ] because the subunit induces cellular uptake of the toxin and not all V. cholerae isolates are toxigenic [ 135 ]. Label-based detection of CT can be achieved using antibodies, ganglioside GM1 (the binding site of CT), or β-galactose, a sugar with strong affinity towards CT. Ahn et al [ 130 ] provide a nice summary of CT-B detection and reported a fluorescence resonance energy transfer (FRET) based method with a theoretical detection limit of 280 pM.…”

Section: Analytesmentioning

confidence: 99%

Nanomaterial enabled sensors for environmental contaminants

2018

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The need and desire to understand the environment, especially the quality of one’s local water and air, has continued to expand with the emergence of the digital age. The bottleneck in understanding the environment has switched from being able to store all of the data collected to collecting enough data on a broad range of contaminants of environmental concern. Nanomaterial enabled sensors represent a suite of technologies developed over the last 15 years for the highly specific and sensitive detection of environmental contaminants. With the promise of facile, low cost, field-deployable technology, the ability to quantitatively understand nature in a systematic way will soon be a reality. In this review, we first introduce nanosensor design before exploring the application of nanosensors for the detection of three classes of environmental contaminants: pesticides, heavy metals, and pathogens.

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Section: Analytesmentioning

confidence: 99%

Nanomaterial enabled sensors for environmental contaminants

2018

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“…A simple and fast bioassay has been developed by Lee et al [83] to recognize cholera toxin (CT), a protein secreted by the Vibrio cholerae bacterium which is responsible for cholera disease. A thiol-modified β-galactose derivative was used to coat 10 nm AuNPs and mixed with amino-terminated quantum dots (CdTe, QD).…”

Section: Reviewmentioning

confidence: 99%

Glyco-gold nanoparticles: synthesis and applications

Compostella¹,

Pitirollo²,

Silvestri³

et al. 2017

Beilstein J. Org. Chem.

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Glyco-gold nanoparticles combine in a single entity the peculiar properties of gold nanoparticles with the biological activity of carbohydrates. The result is an exciting nanosystem, able to mimic the natural multivalent presentation of saccharide moieties and to exploit the peculiar optical properties of the metallic core. In this review, we present recent advances on glyco-gold nanoparticle applications in different biological fields, highlighting the key parameters which inspire the glyco nanoparticle design.

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“…In the FRET system, AuNPs are able to quench the fluorescence of fluorescent materials when a spectral overlap between the absorption spectrum of AuNPs and the emission spectrum of fluorescent materials exists [11,12,13]. Thus, the construction of fluorescent materials for L-Cys sensing is designed to be “off-on” [10,14,15,16,17]. However, the fluorescence spectrum band of traditional fluorescent materials including rhodamine B, gold nanoclusters (AuNCs) or silica nanoparticles is relatively broad and asymmetric [18,19,20], and the effective overlap of the absorbance spectrum of AuNPs or the fluorescence spectrum band of traditional fluorophores is narrow, which affects the detection sensitivity of the immunosensor.…”

Section: Introductionmentioning

confidence: 99%

“…There is a wide overlap of the absorbance spectrum of AuNPs and the fluorescence emission wavelength of fluorescent quantum dots (QDs), but the method is limited by a need for well-trained professionals and the high cost of QDs. Furthermore, some disadvantages are still retained such as cytotoxicity as a result of the release of heavy metal ions, strong dependence of the photoluminescence (PL) on the surface states, as well as chemical and colloidal instabilities of the QDs in harsh environments [14,21,22]. Silicon nanoparticles (SiNPs) are recognized as one type of important semiconductor nanomaterial to overcome these drawbacks.…”

Section: Introductionmentioning

confidence: 99%

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

Zhang

Jia

2017

Sensors

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A new type of fluorescence “off-on” probe was designed for L-Cysteine (L-Cys) based on the fluorescence resonance energy transfer (FRET) between negatively charged amino-capped porous silicon nanoparticles (SiNPs) and positively charged citrate-stabilized Au nanoparticles (AuNPs). In this proposed FRET immunosensor, novel water-soluble amino-conjugated porous SiNPs in ethanol with excellent photoluminescence properties act as the energy donor. Excellent quenching efficiency between SiNPs-ethanol and citrate-stabilized AuNPs by electrostatic interaction via FRET provides an ideal “off-state” (turn-off). The addition of L-Cys leads to releasing the adsorbed AuNPs from the surface of SiNPs and hence the fluorescence emission of SiNPs-ethanol is restored (turn-on), which means the coordination ability of the thiols with AuNPs is stronger than that of the electrostatic interaction. The fluorescence intensity of SiNPs-AuNPs in ethanol is sensitive to L-Cys, and such a restored fluorescence is proportional to the concentration of L-Cys. The method will broadly benefit the development of a new thiol biosensor based on nanostructured porous materials, and the proposed procedure is also expected to develop a variety of functional nanoparticles to form other novel kinds of nanosensors.

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Fluorescence energy transfer inhibition bioassay for cholera toxin based on galactose-stabilized gold nanoparticles and amine-terminated quantum dots

Cited by 24 publications

References 35 publications

Nanomaterial enabled sensors for environmental contaminants

Nanomaterial enabled sensors for environmental contaminants

Glyco-gold nanoparticles: synthesis and applications

Development of Fluorescent FRET Probes for “Off-On” Detection of L-Cysteine Based on Gold Nanoparticles and Porous Silicon Nanoparticles in Ethanol Solution

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