Fluorescence imaging of biochemical relationship between ubiquitinated histone 2A and Polycomb complex protein BMI1

Storti, Barbara; Civita, Simone; Faraci, Paolo; Maroni, Giorgia; Krishnan, Indira; Bizzarri, Ranieri

doi:10.1016/j.bpc.2019.106225

Cited by 11 publications

(8 citation statements)

References 34 publications

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“…A novel alternative to FWHM to determine the resolution is the Fourier Ring Correlation analysis technique, previously developed for cryoelectron microscopy and super‐resolution microscopy. Although not used in this study, it does have advantages for deconvolution and increasing the SNR (Koho et al, ; Storti et al, ).…”

Section: Discussionmentioning

confidence: 99%

Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan

Deroubaix

Moahla

Penny

2020

Microscopy Res & Technique

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The aim of this study was to assess nucleo-cytoplasmic protein localization to better understand the exact intracellular localization of viral proteins involved with infections. Having determined the general protein localization of hepatitis B virus P22 precore protein, the aim was to more specifically resolve its intracellular organization.This was done using both laser scanning microscopy and Airyscan techniques. Using a 63× objective, the resolution obtained with Airyscan was increased by 1.5-fold as compared to confocal microscopy (p value <.00001). K E Y W O R D SAiryscan, confocal, half width full maximum, hepatitis B P22, line profile analysis, resolution

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Section: Discussionmentioning

confidence: 99%

Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan

Deroubaix

Moahla

Penny

2020

Microscopy Res & Technique

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“…BMI1 is the key regulatory component of the epigenetic Polycomb Repressive Complex-1 (PRC1). We recently demonstrated, at the single nucleus level, that the association of BMI1 to PRC1 is needed for its E3-ligase activity in NSCLC cells [369]. The epigenetic role of BMI1 in NSCLC is particularly intriguing since its overexpression drives stem-like properties associated with induction of EMT, resulting in poor prognosis [370].…”

Section: Targeting the Cancer Stem Cell Compartmentmentioning

confidence: 99%

EGFR signaling pathway as therapeutic target in human cancers

Maroni

Tenen

2022

Seminars in Cancer Biology

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133

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“…The simplest model of PcG activity involves trimethylation of H3 by PRC2 at target gene promoters (Blackledge et al, 2015). These epigenetic marks recruit PRC1 on DNA, which in turn acts as E3ligase and ubiquitinates nearby H2A histones (Storti et al, 2019), triggering silencing of gene transcription by local and reversible compaction of chromatin (Illingworth, 2019). The catalytic subunit of PRC2 is constituted by the EZH2 protein.…”

Section: Transcriptional Post-transcriptional and Post-translationamentioning

confidence: 99%

ACE2 in the Era of SARS-CoV-2: Controversies and Novel Perspectives

Saponaro

Rutigliano

Sestito

et al. 2020

Front. Mol. Biosci.

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Angiotensin-converting enzyme 2 (ACE2) is related to ACE but turned out to counteract several pathophysiological actions of ACE. ACE2 exerts antihypertensive and cardioprotective effects and reduces lung inflammation. ACE2 is subjected to extensive transcriptional and post-transcriptional modulation by epigenetic mechanisms and microRNAs. Also, ACE2 expression is regulated post-translationally by glycosylation, phosphorylation, and shedding from the plasma membrane. ACE2 protein is ubiquitous across mammalian tissues, prominently in the cardiovascular system, kidney, and intestine. ACE2 expression in the respiratory tract is of particular interest, in light of the discovery that ACE2 serves as the initial cellular target of severe acute respiratory syndrome (SARS)-coronaviruses, including the recent SARS-CoV2, responsible of the COronaVIrus Disease 2019 (COVID-19). Since the onset of the COVID-19 pandemic, an intense effort has been made to elucidate the biochemical determinants of SARS-CoV2-ACE2 interaction. It has been determined that SARS-CoV2 engages with ACE2 through its spike (S) protein, which consists of two subunits: S1, that mediates binding to the host receptor; S2, that induces fusion of the viral envelope with the host cell membrane and delivery of the viral genome. Owing to the role of ACE2 in SARS-CoV2 pathogenicity, it has been speculated that medical conditions, i.e., hypertension, and/or drugs, i.e., ACE inhibitors and angiotensin receptor blockers, known to influence ACE2 density could alter the fate of SARS-CoV-2 infection. The debate is still open and will only be solved when results of properly designed experimental and clinical investigations will be made public. An interesting observation is, however that, upon infection, ACE2 activity is reduced either by downregulation or by shedding. These events might precipitate the so-called "cytokine storm" that characterizes the most severe COVID-19 forms. As evidence accumulates, ACE2 appears a druggable target in the attempt to limit virus entry and replication. Strategies aimed at blocking ACE2 with antibodies, small molecules or peptides, or at neutralizing the virus by competitive binding with exogenously administered ACE2, are currently under investigations. In this review, we will present an overview of the state-of-the-art knowledge on ACE2 biochemistry and pathophysiology, outlining open issues in the context of COVID-19 disease and potential experimental and clinical developments.

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Fluorescence imaging of biochemical relationship between ubiquitinated histone 2A and Polycomb complex protein BMI1

Cited by 11 publications

References 34 publications

Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan

Monitoring of intracellular localization of Hepatitis B virus P22 protein using Laser Scanning Confocal Microscopy and Airyscan

EGFR signaling pathway as therapeutic target in human cancers

ACE2 in the Era of SARS-CoV-2: Controversies and Novel Perspectives

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