Fluorescence imaging of electrode-solution interfacial processes

Engstrom, Royce C.; Ghaffari, Shahrokh; Qu, Hongwei.

doi:10.1021/ac00045a012

Cited by 59 publications

(58 citation statements)

References 22 publications

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“…Visualization of mass transport phenomena in a redox flow battery has been reported by Charleson and co‐workers . Using fluorescence microscopy with pH‐sensitive dyes, the evolution of the pH distribution can be studied in‐operando …”

Section: Figurementioning

confidence: 96%

In‐Operando Mapping of pH Distribution in Electrochemical Processes

et al. 2019

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Section: Figurementioning

confidence: 96%

In‐Operando Mapping of pH Distribution in Electrochemical Processes

et al. 2019

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“…This approachw as developed in the 1990s by Engstrom and co-workers to monitor,w ith aw ide field fluorescencem icroscope,r eactions producing or consuming protons at the level of an electrode. [2][3][4] Following the work reported on the use of confocal Ramanmicroscopy to map molecular diffusion at microelectrodes, [5,6] in 2002 Unwin and colleagues successfully imaged, for the first time, three-dimensional pH gradients at electrode surfaces using ac onfocal laser scanning microscope that improved the axial and lateral resolutions. [7] In this case, the local pH changes due to the electrochemical reduction of benzoquinonet oh ydroquinone weree videnced thankst ot he presence of fluorescein, which displayed ap H-dependentf luorescents ignal.…”

Section: Introductionmentioning

confidence: 99%

Selective Electrochemical Bleaching of the Outer Leaflet of Fluorescently Labeled Giant Liposomes

Jimenez

Challier

Delacotte

et al. 2017

Chemistry A European J

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Electrochemistry and confocal fluorescence microscopy were successfully combined to selectively bleach and monitor the fluorescence of NBD (7-nitrobenz-2-oxa-1,3-diazole)-labeled phospholipids of giant liposomes. Three types of giant unilamellar vesicles have been investigated, the fluorescent phospholipids being localized either mainly on their outer-, inner-, or both inner/outer leaflets. We established that only the fluorescent lipids incorporated in the outer leaflet of the vesicles underwent electrochemical bleaching upon reduction. The relative fluorescence intensity decay was quantified all along the electrochemical extinction through an original fluorescence loss in electrobleaching (FLIE) assay. As expected, the reorganization of the fluorescent phospholipids followed diffusion-driven dynamics. This was also evidenced by comparison with fluorescence loss in photobleaching (FLIP) and the corresponding numerical model. The value of the lateral diffusion coefficient of phospholipids was found to be similar to that obtained by other methods reported in the literature. This versatile and selective bleaching procedure appears reliable to explore important biological and pharmacological issues.

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“…Fluorescence imaging [22,25,27,28] (FI), which is capable of revealing the nature of the surface chemistry along with surface topography around the active sites, has proved particularly useful for real-time monitoring corrosion and interfacial chemical changes at solid electrodes [29] and solid-liquid interface [30,31]. Studies have mainly employed either fluorescent dye added in the solution itself or non-fluorescent agents in the solution that form fluorescers and emit fluorescence after electrochemical reaction at interfaces.…”

Section: Introductionmentioning

confidence: 99%