Fluorescence in situ hybridization analysis of the fos/jun ratio in the ageing brain

Salehi, Mansoor; Barron, Martin J.; Merry, B.J.; Goyns, M.H.

doi:10.1016/s0047-6374(98)00137-7

Cited by 20 publications

(9 citation statements)

References 24 publications

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“…In case of parallel induction of JunB, the c-Fos proteins might prefer this Jun isoform to c-Jun. The inducibility of cFos declines with progressing age (Salehi et al, 1999) and this observation supports the notion that c-Fos expression widely re¯ects the cellular responsiveness at the transcriptional level.…”

Section: Temporospatial Expression In the Brainsupporting

confidence: 80%

AP-1 proteins in the adult brain: facts and fiction about effectors of neuroprotection and neurodegeneration

Herdegen¹,

Waetzig²

2001

Oncogene

187

118

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Section: Temporospatial Expression In the Brainsupporting

confidence: 80%

AP-1 proteins in the adult brain: facts and fiction about effectors of neuroprotection and neurodegeneration

Herdegen¹,

Waetzig²

2001

Oncogene

187

118

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“…Livin protein expression was measured in all samples. To fulfill this purpose, multi-color FISH method was modified and applied for semi-quantitative Immuno-flourecent method [47]. This procedure had been developed to detect expression of more than one protein within the same cell, at the same time [48,49].…”

Section: Resultsmentioning

confidence: 99%

“…The semi-quantitative method used in this study was the same method developed and applied in our previous studies [47][48][49]. We used two antibodies against Livin and GAPDH.…”

Section: Immunostainingmentioning

confidence: 99%

Livin Expression by Semi-quantitative Immuno-flourecent Staining in Hodgkin Lymphoma: A Promising Marker or a Leading Role in Pathogenesis?

Pouya¹

2015

J Cytol Histol

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A novel human inhibitor of apoptosis protein family member, termed Livin, was demonstrated in the pathogenesis of different human malignancies, and also it was studied as a potential treatment target in malignancies. However, there is no report on the Livin expression profile in Hodgkin Lymphoma. In this study, we evaluated the Livin expression in 78 paraffin embedded blocks including 39 staged cases of Hodgkin Lymphoma and 39 control subjects (normal and reactive hyperplasia lymph nodes). Tissue Microarray-based Semi-quantitative Immuno-flourecent Staining was applied for protein expression profiling in both infiltrating non-neoplastic cells (morphologically typical Lymphocytes) and neoplastic cells (Hodgkin and Reed-Sternberg) of cases and control samples. Our results demonstrated that the mean ratio of Livin/GAPDH expression was significantly increased between infiltrating background cells in Hodgkin Lymphomas and control cases (0.54596 vs. 0.50827, P<0.001). Also, a significant difference was found in the mean ratio of Livin/GAPDH expression between neoplastic cells and major background cells in the tumor microenvironment (0.59024 vs. 0.54596, p<0.001). Furthermore, this study confirmed a significant increase of Livin expression from early-stage to advanced-stage of Hodgkin Lymphoma (0.52888 vs. 0.580146, P<0.01).These findings suggest that Livin may play a critical role in the pathogenesis of Hodgkin Lymphoma. It also can be a novel prognostic marker and a potential therapeutic target in this type of lymphoma.

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“…As no evidence so far exists that expression of one ribosomal protein in mammalian cells can influence the expression of another, the differential expression of a particular ribosomal protein gene during ageing may be more dependent on altered transcription factor activity. It is clear that some transcription factors, such as AP-1 [22,23] and NFÎB [24], exhibit age-related changes in their composition and DNA-binding activity. An implication from these observations is that the level of activity of certain genes may be dependent on the degree to which transcription factors exhibit altered activity during ageing.…”

Section: Resultsmentioning

confidence: 99%

Increased Expression of the S25 Ribosomal Protein Gene Occurs during Ageing of the Rat Liver

Lavery

Goyns

2002

Gerontology

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Background: It appears that consistent changes in the levels of activity of a small cohort of genes (probably <1% of all active genes) occur in all mammalian cells during ageing. Identification of such genes could provide valuable insights into the ageing process. Objective: We have studied age-related changes in gene expression profiles in the rat liver. One of the genes which exhibited clear and consistent increases in expression is characterised. Methods: Analysis of the gene expression profile was carried out using a combination of an optimised form of differential display and single-strand conformational polymorphism (SSCP) gel analysis. Gene expression levels were quantified by Northern blot analysis. The gene’s identity was determined by comparing its nucleotide sequence to DNA databases. Results: During this investigation we observed one gene which exhibited an increase in expression in livers from young adult (6 months) to old adult (24 months) rats. The differential expression of this gene was confirmed by SSCP gel analysis and Northern blotting. Densitometry of the latter indicated that expression increased by 165% with age. Characterisation of the isolated polymerase chain reaction fragment demonstrated it to code for the ribosomal protein S25 (RPS25). Conclusion: This increase in RPS25 expression is further evidence that the composition of ribosomes may alter with age and as a result could have significant effects on protein synthesis.

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Fluorescence in situ hybridization analysis of the fos/jun ratio in the ageing brain

Cited by 20 publications

References 24 publications

AP-1 proteins in the adult brain: facts and fiction about effectors of neuroprotection and neurodegeneration

AP-1 proteins in the adult brain: facts and fiction about effectors of neuroprotection and neurodegeneration

Livin Expression by Semi-quantitative Immuno-flourecent Staining in Hodgkin Lymphoma: A Promising Marker or a Leading Role in Pathogenesis?

Increased Expression of the S25 Ribosomal Protein Gene Occurs during Ageing of the Rat Liver

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