Fluorescence in situ hybridization with multiple repeated DNA probes applied to the analysis of wheat-rye chromosome pairing

Cuadrado, Ángeles; Vitellozzi, Francesca; Jouve, N.; Ceoloni, Carla

doi:10.1007/s001220050422

Cited by 55 publications

(42 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In particular, the nonrecombinant A. aurea chromosome 1 in BC1-3 and BC1-4 was frequently associated with the homoeologous chromosome 1 from A. inodora (Table 1). In A. aurea and A. inodora, chromosome 1 is large compared to other chromosomes (Buitendijk et al, 1997); therefore chromosome size seems to influence the pairing frequency, as was shown in wheat (Bernardo et al, 1988;Cuadrado et al, 1997).…”

Section: Implications For Introgression Of Alien Chromosomesmentioning

confidence: 80%

Meiotic behaviour of individual chromosomes in allotriploid Alstroemeria hybrids

et al. 2004

View full text Add to dashboard Cite

Chromosome association and chiasma formation were studied in pollen mother cells at metaphase I of four allotriplod BC1 plants (2n ¼ 3x ¼ 24) obtained from the backcross of the hybrid Alstroemeria aurea Â A. inodora with its parent A. inodora. We distinguished the chromosomes of both parental species by genomic in situ hybridization (GISH), whereas the individual chromosomes were identified on the basis of their multicolour FISH banding patterns obtained after a second hybridization with two species-specific satellite repeats as probes. All the four BC1 plants possessed two genomes of A. inodora and one of A. aurea. Variable numbers of recombinant chromosomes, resulting from meiotic recombination in the interspecific hybrid, were present in these plants. The homologous A. inodora chromosomes generally formed bivalents, leaving the homoeologous A. aurea chromosomes unassociated. High frequencies of trivalents were observed for the chromosome sets that contained recombinant chromosomes, even when the recombinant segments were small. Chromosome associations in the trivalents were restricted to homologous segments. The implications of the absence of homoeologous chromosome pairing on gamete constitution and prospects for introgression in Alstroemeria are discussed.

show abstract

Section: Implications For Introgression Of Alien Chromosomesmentioning

confidence: 80%

Meiotic behaviour of individual chromosomes in allotriploid Alstroemeria hybrids

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Our results showed that GISH offers a reliable means to discriminate between the identity of chromosomes involved in pairing, using genomic DNA as a probe. GISH provides a great opportunity to unravel the secret of chromosome pairing between different species (Cuadrado et al 1997, Benavante et al 1996. GISH analysis showed that the frequency of recombination is very low.…”

Section: Resultsmentioning

confidence: 99%

Detecting Nature of Chromosome Pairing in A and E Genomes of Oryza

et al. 2009

View full text Add to dashboard Cite

Summary Genomic in situ hybridization (GISH), using genomic DNA probe from O. australiensis, was used to study chromosome pairing among AA, EE and AE genomes, in the hybrid O. sativaϫO. australiensis. In the conventional cytogenetic analysis, 0-4 bivalents and 20-24 univalents were recorded. GISH, however, revealed 1-5 bivalents and 19-23 univalents. 3 types of pairing were detected: pairing between A and E genome chromosomes, within AA genome chromosomes and within EE genome chromosomes. The frequency of association between O. sativa (AA) and O. australiensis (EE) chromosomes (0.98II/cell) greatly exceeded the level of pairing, within sativa chromosomes (0.15II/cell) or within australiensis chromosomes (0.05II/cell). Results indicated that conventional cytogenetic analysis either underestimates or overestimates the pairing behavior and that GISH is a powerful tool for detecting the nature of pairing in O. sativaϫO. australiensis.

show abstract

“…The rye genome has potential as a gene resource for future improvement of wheat. Rye-specific repeated sequences have been useful as probes to determine alien chromatins and chromosome constitutions in wheatrye cross-breeding (Guidet et al 1991, Cuadrado et al 1997. In this study, the SSAP procedure based on Revolver Fig.…”

Section: Usefulness Of Revolver's Biodiversitymentioning

confidence: 99%

Quantitative variation of Revolver transposon-like genes in synthetic wheat and their structural relationship with the LARD element

2009

View full text Add to dashboard Cite

Revolver is a multi-gene family dispersed through Triticeae genomes like transposons. Revolver is similar to class II transposable elements and shows considerable quantitative variation in wheat and its relatives. The highest copy number of Revolver is found in Secale cereale (RR) and the lowest in hexaploid wheat Triticum aestivum (AABBDD). In this study, Revolver copy numbers were determined in synthetic hexaploid wheat lines from crosses between Aegilops tauschii (DD) and T. turgidum tetraploid wheat species (T. dicoccoides, T. dicoccum, T. carthlicum and T. durum, AABB). Eight out of 18 lines showed significantly lower copies than the sum of their parents and seven lines were equal to the sum, suggesting that polyploidy caused loss of Revolver. Members of the Revolver family also showed structural variation in the 5′ region, especially in length. Revolver did not share any similarity with autonomous transposable elements. However, the long terminal repeats (LTRs) of the non-autonomous large retrotransposon derivative (LARD) in barley, showed 60% homology to both 5′ and 3′ ends of some variants of Revolver. LARD LTRs lack the Revolver region from the first exon to the middle of the first intron resulting in non-coding sequences. Evolutionary relationships between Revolver and LARD are discussed.

show abstract

Fluorescence in situ hybridization with multiple repeated DNA probes applied to the analysis of wheat-rye chromosome pairing

Cited by 55 publications

References 26 publications

Meiotic behaviour of individual chromosomes in allotriploid Alstroemeria hybrids

Meiotic behaviour of individual chromosomes in allotriploid Alstroemeria hybrids

Detecting Nature of Chromosome Pairing in A and E Genomes of Oryza

Quantitative variation of Revolver transposon-like genes in synthetic wheat and their structural relationship with the LARD element

Contact Info

Product

Resources

About