Fluorescence line narrowing spectrometric analysis of benzo[a]pyrene-DNA adducts formed by one-electron oxidation

Zamzow, Daniel S.; Jankowiak, Ryszard; Small, Gerald J.; Tibbels, S. R.; Cremonesi, Paolo; Devanesan, Prabu; Rogan, Eleanor G.; Cavalieri, Ercole L.

doi:10.1021/tx00007a005

Cited by 25 publications

(22 citation statements)

References 14 publications

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“…[12][13][14][15][16][17][18][19][20][21] The essential idea behind this technique is that the contribution of the pseudo-PSB to the measured FLN signal can be effectively eliminated by taking the difference between two consecutively measured FLN spectra separated by a period of HB. [12][13][14][15][16][17][18][19][20][21] The essential idea behind this technique is that the contribution of the pseudo-PSB to the measured FLN signal can be effectively eliminated by taking the difference between two consecutively measured FLN spectra separated by a period of HB.…”

Section: Introductionmentioning

confidence: 99%

“…This process was originally described theoretically by Jaaniso 13 whose findings were corroborated by Fünfschilling and co-workers 15 using model calculations; in both cases it was demonstrated that for low burn fluences the contribution of the pseudo-PSB to the measured difference spectrum should be all but eliminated. [16][17][18][19] A similar approach has been used for eliminating fluorescence from nonresonant molecules in single-molecule experiments. [16][17][18][19] A similar approach has been used for eliminating fluorescence from nonresonant molecules in single-molecule experiments.…”

Section: Introductionmentioning

confidence: 99%

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Accurate modeling of fluorescence line narrowing difference spectra: Direct measurement of the single-site fluorescence spectrum

Reppert

Naibo

Jankowiak

2010

The Journal of Chemical Physics

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Accurate lineshape functions for modeling fluorescence line narrowing (FLN) difference spectra (DeltaFLN spectra) in the low-fluence limit are derived and examined in terms of the physical interpretation of various contributions, including photoproduct absorption and emission. While in agreement with the earlier results of Jaaniso [Proc. Est. Acad. Sci., Phys., Math. 34, 277 (1985)] and Funfschilling et al. [J. Lumin. 36, 85 (1986)], the derived formulas differ substantially from functions used recently [e.g., M. Ratsep et al., Chem. Phys. Lett. 479, 140 (2009)] to model DeltaFLN spectra. In contrast to traditional FLN spectra, it is demonstrated that for most physically reasonable parameters, the DeltaFLN spectrum reduces simply to the single-site fluorescence lineshape function. These results imply that direct measurement of a bulk-averaged single-site fluorescence lineshape function can be accomplished with no complicated extraction process or knowledge of any additional parameters such as site distribution function shape and width. We argue that previous analysis of DeltaFLN spectra obtained for many photosynthetic complexes led to strong artificial lowering of apparent electron-phonon coupling strength, especially on the high-energy side of the pigment site distribution function.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Accurate modeling of fluorescence line narrowing difference spectra: Direct measurement of the single-site fluorescence spectrum

Reppert

Naibo

Jankowiak

2010

The Journal of Chemical Physics

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“…29C). This is also the case for the C8Gua and C8dG one-electron oxidation adducts of B[a]P [153]. The vibrational frequencies for V-MBA-lZ-CH^-CBGua are listed in Table 3, column 3.…”

Section: Fln Spectra Of Isomeric Bptsmentioning

confidence: 70%

“…As in Figure 32, these wavelengths were chosen to reveal mode structure in the 600 cm"^ and 1200 cm"^ regions. Spectra a in Figure 33A and B were chosen to reveal mode structure of the standard BP-6-C8Gua adduct and are identical to the FLN spectra reported earlier [153]. The strongest mode in spectrum A is now 498 cm"^ compared to 588 cm"^ in Figure 32A Finally, a depurination adduct identified was BPDE-N7Ade, which are formed by monooxygenation.…”

Section: Wavelength (Nm)mentioning

confidence: 85%

“…The selectivity was, however, improved by the application of double spectral selection [150] which was discussed in the section H-C. The line-narrowed double selection FLN spectra of the above three adducts exhibited minor differences which were used to prove that a major depurination adduct from the binding of B[a]P to DNA in rat liver nuclei was BP-6-N7Gua [153]. The major adduct from metabolic activation of B[a]P by one-electron oxidation in the horseradish peroxidase catalyzed reaction of B[a]P with DNA (in vitro) was also identified as BP-6-C8Gua [153].…”

Section: B Instrumentationmentioning

confidence: 99%

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High resolution fluorescence studies of metabolic pathways of DNA and oligonucleotide adducts from polycyclic aromatic compounds

Jeong

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UMI MICROFILMED 1991 INFORMATION TO USERSThis manuscript has been reproduced from the microfflm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer.The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction.In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion.Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand corner and continuing from left to right in equal sections with small overlaps. Each original is also photographed in one exposure and is included in reduced form at the back of the book.Photographs included in the original manuscript have been reproduced xerographically in this copy. Higher quality 6" x 9" black and white photographic prints are available for any photographs or illustrations appearing in this copy for an additional charge. Contact UMI directly to order. 1991Signature was redacted for privacy.Signature was redacted for privacy.Signature was redacted for privacy.ii Ultraviolet and X-ray light have long beeHxknown to be highly cancer-causing and mutagenic agents [16]. For example, exposure of skin to UV light can induce skin cancer, while X-ray exposure to the thyroid induces thyroid cancer. Electromagnetic radiation, however, may not be assumed to cause all the cells in an exposed population to become cancerous since radiation can also produce many other deleterious changes leading to cell death. A radiation dose sufficient to induce cancer in all cells will virtually kill every cell. Not only can radiation induce simple mutation, it can also produce chromosome fragmentation, resulting in large deletions of genetic material. Thus radiation causes a variety of genetic changes, many of which might contribute to the cancerous state. A well known deleterious effect, xeroderma pigmentosum, is magnified in certain individuals. These people are homozygous for a recessive mutation that inactivates a gene involved in repairing thymine dimers, the major damage caused by UV Similar studies have shown that relatively weak carcinogens are also metabolized by monooxgenation mechanism. In most cases, however, the evidence is much less complete than in the case of B[a]P and rests primarily on comparison of the relative mutagenicities and tumorigenicities of the isomeric dihydrodiols or the corresponding diol epoxides obtained through synthesis. One-electron oxidation and metabolitesThe monooxygenation mechanism, which emphasizes the important role of the bay-region diol epoxide, has been widely accepted during last 15 years...

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Fluorescence line-narrowing spectrometry: A versatile tool for the study of chemically initiated carcinogenesis

Jankowiak

Small

et al. 1990

Journal of Pharmaceutical and Biomedical Analysis

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Fluorescence line narrowing spectrometric analysis of benzo[a]pyrene-DNA adducts formed by one-electron oxidation

Cited by 25 publications

References 14 publications

Accurate modeling of fluorescence line narrowing difference spectra: Direct measurement of the single-site fluorescence spectrum

Accurate modeling of fluorescence line narrowing difference spectra: Direct measurement of the single-site fluorescence spectrum

High resolution fluorescence studies of metabolic pathways of DNA and oligonucleotide adducts from polycyclic aromatic compounds

Fluorescence line-narrowing spectrometry: A versatile tool for the study of chemically initiated carcinogenesis

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